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GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a "zinc finger" sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
antigen NY-CO-27; GAL4; Gal-4; GAL81; galactose-responsive transcription factor GAL4; galectin 4; galectin-4; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; lectin, galactoside binding soluble 4; lectin, galactoside-binding, soluble, 4; LGALS4; Regulatory protein GAL4; YPL248C
200 µg
100 µg