Invitrogen

CD15 Monoclonal Antibody (HI98), FITC, eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4个已发表图片

14 篇参考文献

查看其他65个SSEA1 (CD15)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 7

CD15 Antibody (11-0159-42) in Flow

THP-1 and Ramos cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-000) and then stained with CD15 Monoclonal Antibody (HI98), FITC, eBioscience™ (Product # 11-0159-42) at a concentration of 0.5 µg (green histogram) and CD19 Monoclonal Antibody (HIB19), APC, eBioscience™ (Product # 17-0199-42) (red histogram) at a concentration of 1 µg or left unstained (black histogram). CD15 and CD19 were mutually exclusively present on THP-1 and Ramos cells respectively. Viable cells, as ... View More

CD15 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

CD15 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD15 was observed in THP-1 in comparison to low to negative in Ramos using CD15 Monoclonal Antibody (HI98), FITC, eBioscience™ (Product # 11-0159-42). {RE}

产品信息

11-0159-42

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgM, kappa

分类

Monoclonal

类型

Antibody

克隆号

HI98

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11040984

产品详细信息

Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.

Applications Reported: The HI98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Increase of leucocyte-derived extracellular traps (ETs) in semen samples from human acute epididymitis patients-a pilot study.

Journal of assisted reproduction and genetics

Zambrano F,Schulz M,Pilatz A,Wagenlehner F,Schuppe HC,Conejeros I,Uribe P,Taubert A,Sánchez R,Hermosilla C

11-0159-42 was used in Immunocytochemistry to demonstrate the presence of neutrophilic extracellular traps used in ETosis during acute epididymitis.

Tue Sep 01 00:00:00 EDT 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

A high-risk luminal A dominant breast cancer subtype with increased mobility.

Breast cancer research and treatment

Guo L,Chen G,Zhang W,Zhou L,Xiao T,Di X,Wang Y,Feng L,Zhang K

Published figure using CD15 monoclonal antibody (Product # 11-0159-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Label-free optical hemogram of granulocytes enhanced by artificial neural networks.

Optics express

Gupta RK,Chen M,Malcolm GPA,Hempler N,Dholakia K,Powis SJ

11-0159-42 was used in Flow cytometry/Cell sorting to demonstrate a comparison between artificial neural networks and principal component analysis to classify the key granulocyte cell lineages of neutrophils and eosinophils.

Mon May 13 00:00:00 EDT 2019

Multiomics Analyses of HNF4α Protein Domain Function during Human Pluripotent Stem Cell Differentiation.

iScience

Wang Y,Tatham MH,Schmidt-Heck W,Swann C,Singh-Dolt K,Meseguer-Ripolles J,Lucendo-Villarin B,Kunath T,Rudd TR,Smith AJH,Hengstler JG,Godoy P,Hay RT,Hay DC

11-0159-42 was used in Flow Cytometry to demonstrate that nuclear HNF4α is essential for hepatic progenitor specification, and the introduction of point mutations in HNF4α's Small Ubiquitin-like Modifier consensus motif leads to disrupted hepatocyte differentiation.

Fri Jun 28 00:00:00 EDT 2019

Stimulation of medulloblastoma stem cells differentiation by a peptidomimetic targeting neuropilin-1.

Oncotarget

Gong C,Valduga J,Chateau A,Richard M,Pellegrini-Moïse N,Barberi-Heyob M,Chastagner P,Boura C

Published figure using CD15 monoclonal antibody (Product # 11-0159-42) in Western Blot

Fri Mar 16 00:00:00 EDT 2018

Direct Activation of BAX by BTSA1 Overcomes Apoptosis Resistance in Acute Myeloid Leukemia.

Cancer cell

Reyna DE,Garner TP,Lopez A,Kopp F,Choudhary GS,Sridharan A,Narayanagari SR,Mitchell K,Dong B,Bartholdy BA,Walensky LD,Verma A,Steidl U,Gavathiotis E

11-0159 was used in Flow cytometry/Cell sorting to identify BTSA1, a pharmacologically optimised BAX activator that binds with high affinity and specificity to the N-terminal activation site and induces conformational changes to BAX leading to BAX-mediated apoptosis.

Mon Oct 09 00:00:00 EDT 2017

A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model.

PloS one

Yuan X,Li Z,Baines AC,Gavriilaki E,Ye Z,Wen Z,Braunstein EM,Biesecker LG,Cheng L,Dong X,Brodsky RA

11-0159 was used in Flow cytometry/Cell sorting to provide direct evidence for a critical role of glycosylphosphatidylinositol anchor proteins in early neurodevelopment.

Wed Sep 06 00:00:00 EDT 2017

Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia.

The Journal of clinical investigation

Ding L,Hayes MM,Photenhauer A,Eaton KA,Li Q,Ocadiz-Ruiz R,Merchant JL

Published figure using CD15 monoclonal antibody (Product # 11-0159-42) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2016

Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia.

The Journal of clinical investigation

Ding L,Hayes MM,Photenhauer A,Eaton KA,Li Q,Ocadiz-Ruiz R,Merchant JL

Published figure using CD15 monoclonal antibody (Product # 11-0159-42) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2016

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

11-0159 was used in Flow cytometry/Cell sorting to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 EDT 2016

TNFα promotes CAR-dependent migration of leukocytes across epithelial monolayers.

Scientific reports

Morton PE,Hicks A,Ortiz-Zapater E,Raghavan S,Pike R,Noble A,Woodfin A,Jenkins G,Rayner E,Santis G,Parsons M

11-0159 was used in Flow cytometry/Cell sorting to demonstrate that trans-epithelial migration of monocyte-derived THP-1 cells is regulated by phosphorylation of the Coxsackie and Adenovirus receptor.

Thu May 19 00:00:00 EDT 2016

Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis.

Journal of immunology (Baltimore, Md. : 1950)

Bilwani FA,Knight KL

11-0159 was used in Flow cytometry/Cell sorting to investigate the factors contributing to an observed age-related decline in B lymphopoiesis.

Thu Nov 01 00:00:00 EDT 2012

Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Journal of leukocyte biology

Unsinger J,McDonough JS,Shultz LD,Ferguson TA,Hotchkiss RS

11-0159 was used in Flow cytometry/Cell sorting to develop a humanised mouse model that recapitulates many of the classic findings in patients with sepsis.

Sat Aug 01 00:00:00 EDT 2009

生物信息学

蛋白别名: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

查看更多收起

基因别名: CD15; ELFT; FCT3A; FUC-TIV; FUT4; FUTIV; LeX; SSEA-1

查看更多收起

UniProt ID: (Human) P22083

查看更多收起

Entrez Gene ID: (Human) 2526

查看更多收起

功能

fucosyltransferase activity alpha-(1->3)-fucosyltransferase activity glycosyltransferase

参与通路

carbohydrate metabolic process protein glycosylation protein N-linked glycosylation fucosylation L-fucose catabolic process