Invitrogen

IL-13 Monoclonal Antibody (85BRD), FITC, eBioscience™

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IL-13 Antibody (11-7136-42) in Flow

Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were stained intracellularly with Anti-Human CD4 PE (Product # 12-0049-42) and Anti-Human IL-13 FITC using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

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产品信息

11-7136-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Human, Nematode

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

85BRD

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572515

产品详细信息

Description: The 85BRD monoclonal antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.

Applications Reported: This 85BRD antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 85BRD antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Antigen-Specific Adaptive Immunity to SARS-CoV-2 in Acute COVID-19 and Associations with Age and Disease Severity.

Cell

Rydyznski Moderbacher C,Ramirez SI,Dan JM,Grifoni A,Hastie KM,Weiskopf D,Belanger S,Abbott RK,Kim C,Choi J,Kato Y,Crotty EG,Kim C,Rawlings SA,Mateus J,Tse LPV,Frazier A,Baric R,Peters B,Greenbaum J,Ollmann Saphire E,Smith DM,Sette A,Crotty S

11-7136 was used in Flow cytometry/Cell sorting to complete a combined examination of all three branches of adaptive immunity at the level of SARS-CoV-2-specific CD4+ and CD8+ T cell and neutralizing antibody responses in acute and convalescent subjects.

Thu Nov 12 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

11-7136 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

11-7136 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 EST 2020

CD4⁺ T_h immunogenicity of the Ascaris spp. secreted products.

NPJ vaccines

Ebner F,Morrison E,Bertazzon M,Midha A,Hartmann S,Freund C,Álvaro-Benito M

11-7136-42 was used in Flow Cytometry to identify the first human T cell epitope against Ascaris spp. and represents an easily adaptable platform for characterization of complex antigens.

Thu Mar 18 00:00:00 EDT 2021

RNA-seq and flow-cytometry of conventional, scalp, and palmoplantar psoriasis reveal shared and distinct molecular pathways.

Scientific reports

Ahn R,Yan D,Chang HW,Lee K,Bhattarai S,Huang ZM,Nakamura M,Singh R,Afifi L,Taravati K,Munoz-Sandoval P,Pauli M,Rosenblum MD,Liao W

Fri Jul 27 00:00:00 EDT 2018

生物信息学

蛋白别名: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody

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基因别名: IL-13; IL13; NC30; P600

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UniProt ID: (Human) P35225

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Entrez Gene ID: (Human) 3596

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功能

cytokine activity interleukin-13 receptor binding protein binding

参与通路