Invitrogen

CD19 Monoclonal Antibody (SJ25C1), PE, eBioscience™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4个已发表图片

16 篇参考文献

查看其他250个CD19抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 7

CD19 Antibody (12-0198-42) in Flow

Knockout of CD19 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR807388_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD19 was performed by staining Raji CD19 knock out cells with 0.06 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-42, yellow histogram) or 0.06 µg CD19 Monoclonal Antibody (SJ25C1), PE, eBioscience (Product # 12-0198-42, blue histogram). Raji Cas9 control cells were also stained with CD1... View More

Product Image Gallery

CD19 Antibody — Antibody clone (SJ25C1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD19 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD19 antibody (SJ25C1). Yellow histogram represents staining with the isotype control. {KO}

产品信息

12-0198-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

查看16篇已发表文章 16篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SJ25C1

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10734045

产品详细信息

Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.

Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SJ25C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Tyrosine Kinase Inhibitors Target B Lymphocytes.

Biomolecules

Upfold NLE,Petakh P,Kamyshnyi A,Oksenych V

Published figure using CD19 monoclonal antibody (Product # 12-0198-42) in Flow Cytometry

Sat Feb 25 00:00:00 EST 2023

Evaluation of peripheral lymphocyte subsets in acne vulgaris patients before and after systemic isotretinoin treatment.

Indian journal of pharmacology

Gunaydin SD,Tezcan I

Published figure using CD19 monoclonal antibody (Product # 12-0198-42) in Flow Cytometry

Wed Dec 21 00:00:00 EST 2022

Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV.

Pathogens (Basel, Switzerland)

Bazié WW,Goyer B,Boucher J,Zhang Y,Planas D,Chatterjee D,Routy JP,Alary M,Ancuta P,Gilbert C

12-0198-42 was used in Flow Cytometry to investigate daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV-).

Sat Apr 24 00:00:00 EDT 2021

Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.

Nature communications

Asano Y,Daccache J,Jain D,Ko K,Kinloch A,Veselits M,Wolfgeher D,Chang A,Josephson M,Cunningham P,Tambur A,Khan AA,Pillai S,Chong AS,Clark MR

Published figure using CD19 monoclonal antibody (Product # 12-0198-42) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.

Nature communications

Asano Y,Daccache J,Jain D,Ko K,Kinloch A,Veselits M,Wolfgeher D,Chang A,Josephson M,Cunningham P,Tambur A,Khan AA,Pillai S,Chong AS,Clark MR

Published figure using CD19 monoclonal antibody (Product # 12-0198-42) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Phase I Study of Ficlatuzumab and Cetuximab in Cetuximab-Resistant, Recurrent/Metastatic Head and Neck Cancer.

Cancers

Bauman JE,Ohr J,Gooding WE,Ferris RL,Duvvuri U,Kim S,Johnson JT,Soloff AC,Wallweber G,Winslow J,Gaither-Davis A,Grandis JR,Stabile LP

12-0198-42 was used in Flow Cytometry to establish the recommended Phase II dose (RP2D). Secondary objectives included overall response rate (ORR), progression-free survival (PFS), and overall survival (OS).

Thu Jun 11 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD19 monoclonal antibody (Product # 12-0198-42) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

Human C. difficile toxin-specific memory B cell repertoires encode poorly neutralizing antibodies.

JCI insight

Shah HB,Smith K,Scott EJ,Larabee JL,James JA,Ballard JD,Lang ML

Published figure using CD19 monoclonal antibody (Product # 12-0198-42) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

Umbilical cord blood‑derived Helios‑positive regulatory T cells promote angiogenesis in acute lymphoblastic leukemia in mice via CCL22 and the VEGFA‑VEGFR2 pathway.

Molecular medicine reports

Li X,Li D,Shi Q,Huang X,Ju X

12-0198 was used in Flow cytometry/Cell sorting to elucidate the mechanisms underlying the role of regulatory T cells in acute lymphoblastic leukaemia.

Wed May 01 00:00:00 EDT 2019

Evaluation of Canonical Inflammasome Activation in Human Monocytes by Imaging Flow Cytometry.

Frontiers in immunology

Lage SL,Dominical VM,Wong CS,Sereti I

12-0198-42 was used in Flow Cytometry to explore the optimal imaging-based tools to measure ASC (apoptosis-associated speck-like protein containing a caspase activating and recruitment domain) speck formation.

Wed Oct 28 00:00:00 EDT 2020

Interferon inducible X-linked gene CXorf21 may contribute to sexual dimorphism in Systemic Lupus Erythematosus.

Nature communications

Odhams CA,Roberts AL,Vester SK,Duarte CST,Beales CT,Clarke AJ,Lindinger S,Daffern SJ,Zito A,Chen L,Jones LL,Boteva L,Morris DL,Small KS,Fernando MMA,Cunninghame Graham DS,Vyse TJ

12-0198-42 was used in Flow cytometry/Cell sorting to reveal modulation in gene expression affected by the combination of two hallmarks of SLE: CXorf21 expression increases in a both an IFN-inducible and sex-specific manner.

Wed May 15 00:00:00 EDT 2019

Multi-Donor Longitudinal Antibody Repertoire Sequencing Reveals the Existence of Public Antibody Clonotypes in HIV-1 Infection.

Cell host & microbe

Setliff I,McDonnell WJ,Raju N,Bombardi RG,Murji AA,Scheepers C,Ziki R,Mynhardt C,Shepherd BE,Mamchak AA,Garrett N,Karim SA,Mallal SA,Crowe JE,Morris L,Georgiev IS

12-0198 was used in Flow cytometry/Cell sorting to reveal the existence of "public" antibody clonotypes that were shared among multiple HIV-infected individuals.

Wed Jun 13 00:00:00 EDT 2018

Bacillus anthracis lethal toxin negatively modulates ILC3 function through perturbation of IL-23-mediated MAPK signaling.

PLoS pathogens

Seshadri S,Allan DSJ,Carlyle JR,Zenewicz LA

12-0198 was used in Flow cytometry/Cell sorting to study the effects of MAPK signalling pathways in group 3 innate immune lymphocytes using Bacillus anthracis.