Invitrogen

CD21 Monoclonal Antibody (HB5), PE, eBioscience™

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CD21 Antibody (12-0219-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (left) or PE Anti-Human CD21 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-0219-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

HB5

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1548734

产品详细信息

Description: The HB5 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein. CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV). CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation.

Applications Reported: This HB5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HB5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to follicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD21 functions as receptor for C3d, C3dg and iC3b complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. Genetic variations in the CD21 gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively, spliced transcript variants encoding different isoforms of CD21 have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

12-0219 was used in Flow cytometry/Cell sorting to uncover a mechanism that controls myeloid regeneration and early lineage decisions in HSCs and could be targeted in LSCs to normalize leukemic myeloid cell production.

Mon Mar 02 00:00:00 EST 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Mon Mar 02 00:00:00 EST 2020

CD11c⁺ B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors.

Frontiers in immunology

Golinski ML,Demeules M,Derambure C,Riou G,Maho-Vaillant M,Boyer O,Joly P,Calbo S

12-0219-42 was used in Flow Cytometry to characterise CD11c+ B cells from healthy donors by flow cytometry, microarray analysis, and in vitro functional assays.

Tue Feb 23 00:00:00 EST 2021

A Molecular Signature in Blood Reveals a Role for p53 in Regulating Malaria-Induced Inflammation.

Immunity

Tran TM,Guha R,Portugal S,Skinner J,Ongoiba A,Bhardwaj J,Jones M,Moebius J,Venepally P,Doumbo S,DeRiso EA,Li S,Vijayan K,Anzick SL,Hart GT,O'Connell EM,Doumbo OK,Kaushansky A,Alter G,Felgner PL,Lorenzi H,Kayentao K,Traore B,Kirkness EF,Crompton PD

12-0219-42 was used in Flow cytometry/Cell sorting to investigate the immune response to malarial infection and p53 activation.

Tue Oct 15 00:00:00 EDT 2019

Epstein-Barr Virus Infection of Mammary Epithelial Cells Promotes Malignant Transformation.

EBioMedicine

Hu H,Luo ML,Desmedt C,Nabavi S,Yadegarynia S,Hong A,Konstantinopoulos PA,Gabrielson E,Hines-Boykin R,Pihan G,Yuan X,Sotiriou C,Dittmer DP,Fingeroth JD,Wulf GM

12-0219 was used in Blocking experiments to show that EBV can infect primary mammary epithelial cells expressing receptor CD21 and contribute to the formation of breast cancer.

Fri Jul 01 00:00:00 EDT 2016

Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function.

eLife

Portugal S,Tipton CM,Sohn H,Kone Y,Wang J,Li S,Skinner J,Virtaneva K,Sturdevant DE,Porcella SF,Doumbo OK,Doumbo S,Kayentao K,Ongoiba A,Traore B,Sanz I,Pierce SK,Crompton PD

12-0219 was used in Flow cytometry/Cell sorting to elucidate the function of atypical memory B cells and their relationship to classical memory B cells.

Fri May 08 00:00:00 EDT 2015

Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function.

eLife

Portugal S,Tipton CM,Sohn H,Kone Y,Wang J,Li S,Skinner J,Virtaneva K,Sturdevant DE,Porcella SF,Doumbo OK,Doumbo S,Kayentao K,Ongoiba A,Traore B,Sanz I,Pierce SK,Crompton PD

12-0219 was used in Flow cytometry/Cell sorting to elucidate the function of atypical memory B cells and their relationship to classical memory B cells.

Fri May 08 00:00:00 EDT 2015

DCLRE1C (ARTEMIS) mutations causing phenotypes ranging from atypical severe combined immunodeficiency to mere antibody deficiency.

Human molecular genetics

Volk T,Pannicke U,Reisli I,Bulashevska A,Ritter J,Björkman A,Schäffer AA,Fliegauf M,Sayar EH,Salzer U,Fisch P,Pfeifer D,Di Virgilio M,Cao H,Yang F,Zimmermann K,Keles S,Caliskaner Z,Güner SÜ,Schindler D,Hammarström L,Rizzi M,Hummel M,Pan-Hammarström Q,Schwarz K,Grimbacher B

12-0219 was used in Flow cytometry/Cell sorting to investigate the range of phenotypes associated with DCLRE1C (ARTEMIS) mutations in humans.

Sun Dec 20 00:00:00 EST 2015

Atypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies.

The Journal of experimental medicine

Muellenbeck MF,Ueberheide B,Amulic B,Epp A,Fenyo D,Busse CE,Esen M,Theisen M,Mordmüller B,Wardemann H

12-0219 was used in Flow cytometry/Cell sorting to study how Plasmodium falciparum neutralizing antibodies are produced by memory B cells.

Mon Feb 11 00:00:00 EST 2013

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

12-0219 was used in Flow cytometry/Cell sorting to expose long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

Fri Nov 01 00:00:00 EDT 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

12-0219 was used in Flow cytometry/Cell sorting to study B and T cell exhaustion caused by chronic exposure to Plasmodium falciparum.

Fri Feb 01 00:00:00 EST 2013

生物信息学

蛋白别名: CD21; Complement C3d receptor; Complement C3d receptor (C3DR); complement component (3d/Epstein Barr virus) receptor 2; Complement receptor type 2; Complement Receptor type 2 (CR2); Cr2; EBV receptor; EBV-R; Epstein-Barr virus receptor; EVBR

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基因别名: C3DR; CD21; CR; CR2; CVID7; SLEB9

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UniProt ID: (Human) P20023

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Entrez Gene ID: (Human) 1380

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功能

virus receptor activity complement binding DNA binding complement receptor activity transmembrane signaling receptor activity protein homodimerization activity

参与通路

complement receptor mediated signaling pathway immune response complement activation, classical pathway B cell differentiation B cell proliferation innate immune response viral entry into host cell