Invitrogen

CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), PE, eBioscience™

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CD43 Antibody (12-0439-42) in Flow

Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, eFluor 450 (Product # 48-0038-42) and Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (left) or CD43 Monoclonal Antibody, PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-0439-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Human

已发表种属

Fish, Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio84-3C1 (84-3C1)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10854419

产品详细信息

Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.

STAR protocols

Garcia-Alegria E,Potts B,Menegatti S,Kouskoff V

Published figure using CD43 monoclonal antibody (Product # 12-0439-42) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model.

Journal of human genetics

Ichisima J,Suzuki NM,Samata B,Awaya T,Takahashi J,Hagiwara M,Nakahata T,Saito MK

Published figure using CD43 monoclonal antibody (Product # 12-0439-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture.

Stem cells translational medicine

Watanabe D,Koyanagi-Aoi M,Taniguchi-Ikeda M,Yoshida Y,Azuma T,Aoi T

12-0439 was used in Flow cytometry/Cell sorting to develop a system for generating induced pluripotent stem cells that harbor rearrangements of TCRG and TCRD gene regions without cell-sorting.

Mon Jan 01 00:00:00 EST 2018

The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture.

Stem cells translational medicine

Watanabe D,Koyanagi-Aoi M,Taniguchi-Ikeda M,Yoshida Y,Azuma T,Aoi T

12-0439 was used in Flow cytometry/Cell sorting to develop a system for generating induced pluripotent stem cells that harbor rearrangements of TCRG and TCRD gene regions without cell-sorting.

Mon Jan 01 00:00:00 EST 2018

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 12-0439-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease.

Biomedical reports

Yoon JY,Lee Y,Yu SL,Yoon HK,Park HY,Joung CI,Park SR,Kwon M,Kang J

Published figure using CD43 monoclonal antibody (Product # 12-0439-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 12-0439-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

12-0439 was used in Flow cytometry/Cell sorting to showcase the therapeutic potential of gene-editing coupled with stem cell technology for the treatment of recessive dystrophic epidermolysis bullosa.

Wed Sep 30 00:00:00 EDT 2020

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD43 monoclonal antibody (Product # 12-0439-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD43 monoclonal antibody (Product # 12-0439-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

Published figure using CD43 monoclonal antibody (Product # 12-0439-42) in Flow Cytometry

Thu Jul 17 00:00:00 EDT 2014

Characterization of proposed human B-1 cells reveals pre-plasmablast phenotype.

Blood

Covens K,Verbinnen B,Geukens N,Meyts I,Schuit F,Van Lommel L,Jacquemin M,Bossuyt X

12-0439 was used in Flow cytometry/Cell sorting to offer perspectives for the investigation of differentiation of B cells into antibody secreting cells.

Thu Jun 27 00:00:00 EDT 2013

生物信息学

蛋白别名: CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115

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基因别名: CD43; GALGP; GPL115; LSN; SPN

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UniProt ID: (Human) P16150

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Entrez Gene ID: (Human) 6693

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功能

transmembrane signaling receptor activity

参与通路