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Mouse IgG3 Isotype Control (12-4742-42) in Flow
Human monocyte derived macrophages were stimulated with IL-10 for 48 hours, and subsequently stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and Mouse IgG3 kappa Isotype Control, PE (Product # 12-4742-42) (left) or MARCO Monoclonal Antibody, PE (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
产品信息
12-4742-42
应用
建议稀释比
流式细胞分析 (Flow)
10 µL/test
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Mouse
/ IgG3, lambda
分类
Monoclonal
类型
Isotype Control
克隆号
B10
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
10 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10733011
产品详细信息
Description: The mouse IgG3 lambda is useful as an isotype control immunoglobulin.
Applications Reported: This mouse IgG3 isotype control has been reported for use in flow cytometric analysis.
Applications Tested: This mouse IgG3 isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 10 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. .
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Mouse IgG3 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the least abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG3 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG3 is less commonly used in research and diagnostic applications compared to other IgG subtypes, but it is still used in some applications such as ELISA and immunohistochemistry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Immunoglobulin G; Immunoglobulin G3; ImmunoglobulinG; ImmunoglobulinG3
