Invitrogen

FOXP3 Monoclonal Antibody (150D/E4), PE, eBioscience™

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FOXP3 Antibody (12-4774-42) in Flow

Surface staining of C57BL/6 splenocytes with Anti-Mouse CD25 APC (Product # 17-0251-82) followed bY Fixation and Permeabilization witt the Foxp3 Buffer Set (Product # 00-5523-00). Cells were subsequently stained with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Foxp3 PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-4774-42

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

150D/E4

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10670338

产品详细信息

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human/primate and mouse/rat Foxp3 protein, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to Foxp3 available from eBioscience, which have been used significantly in scientific literature, include the anti-human Foxp3 PCH101 (Product # 72-5776) and ebio7979 (Product # 12-7979), and the anti-mouse/rat Foxp3 FJK-16s (Product # 72-5775). The 150D/E4 has been mapped to the splice region in Exon 2 found in human cells. The splicing of this region has not been shown to occur in mouse. Characterization of this antibody to the splice variant has been limited to epitope mapping. It should be noted that other antibodies such as PCH101 and 236A/E7 will recognize both the spliced and full length forms of the Foxp3 protein and stain more intensely than 150D/E4 and FJK-16s in human cells.

The 150D/E4 antibody reacts with mouse/rat/human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of FoxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Applications Reported: This 150D/E4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 150D/E4 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Nanoliposome C6-Ceramide in combination with anti-CTLA4 antibody improves anti-tumor immunity in hepatocellular cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Qi X,Wu F,Kim SH,Kaifi JT,Kimchi ET,Snyder H,Illendula A,Fox T,Kester M,Staveley-O'Carroll KF,Li G

12-4774-42 was used in Flow cytometry/Cell sorting to investigate the potential of LipC6 in combination with immune checkpoint blockade in hepatocellular cancer treatment.

Fri Apr 01 00:00:00 EDT 2022

Regulatory T-cell Transcriptomic Reprogramming Characterizes Adverse Events by Checkpoint Inhibitors in Solid Tumors.

Cancer immunology research

Grigoriou M,Banos A,Hatzioannou A,Kloetgen A,Kouzis P,Aggouraki D,Zakopoulou R,Bamias G,Kassi E,Mavroudis D,Bamias A,Boumpas DT,Tsirigos A,Gogas H,Alissafi T,Verginis P

12-4774-42 was used in Flow Cytometry to demonstrate an intense “inflammatory” phenotype in CD4+CD25+CD127- T regulatory cells isolated from the blood of patients with advanced metastatic melanoma who developed immune-related adverse events after immune checkpoint inhibitor therapy.

Thu Jul 01 00:00:00 EDT 2021

Retinoic Acid Induces Functionally Suppressive Foxp3⁺RORγt⁺ T Cells In Vitro.

Frontiers in immunology

Martínez-Blanco M,Lozano-Ojalvo D,Pérez-Rodríguez L,Benedé S,Molina E,López-Fandiño R

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

Retinoic Acid Induces Functionally Suppressive Foxp3⁺RORγt⁺ T Cells In Vitro.

Frontiers in immunology

Martínez-Blanco M,Lozano-Ojalvo D,Pérez-Rodríguez L,Benedé S,Molina E,López-Fandiño R

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Fri Jul 17 00:00:00 EDT 2020

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Fri Jul 17 00:00:00 EDT 2020

Mitochondrial transfer from MSCs to T cells induces Treg differentiation and restricts inflammatory response.

EMBO reports

Court AC,Le-Gatt A,Luz-Crawford P,Parra E,Aliaga-Tobar V,Bátiz LF,Contreras RA,Ortúzar MI,Kurte M,Elizondo-Vega R,Maracaja-Coutinho V,Pino-Lagos K,Figueroa FE,Khoury M

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Wed Feb 05 00:00:00 EST 2020

Differential expression of tissue-restricted antigens among mTEC is associated with distinct autoreactive T cell fates.

Nature communications

Lebel MÈ,Coutelier M,Galipeau M,Kleinman CL,Moon JJ,Melichar HJ

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Fri Jul 24 00:00:00 EDT 2020

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA.

Cell reports

Agarwal S,Kraus Z,Dement-Brown J,Alabi O,Starost K,Tolnay M

12-4774 was used in Flow cytometry/Cell sorting to study the functional regulation of T cells by human Fc receptor-like 3.

Tue Feb 04 00:00:00 EST 2020

Interleukin-24 Regulates T Cell Activity in Patients With Colorectal Adenocarcinoma.

Frontiers in oncology

Zhang Y,Liu Y,Xu Y

12-4774-42 was used in Flow Cytometry to indicate that IL-24 might regulate T cell function in a dose-dependent manner.

Wed Sep 30 00:00:00 EDT 2020

A fully-virulent retargeted oncolytic HSV armed with IL-12 elicits local immunity and vaccine therapy towards distant tumors.

PLoS pathogens

Leoni V,Vannini A,Gatta V,Rambaldi J,Sanapo M,Barboni C,Zaghini A,Nanni P,Lollini PL,Casiraghi C,Campadelli-Fiume G

12-4774 was used in Flow cytometry/Cell sorting to analyse the efficacy of a fully-virulent HER2-retargeted oncolytic HSV, demonstrating its ability to elicit a local immune response and systemic therapy vaccine effects.

Wed Aug 01 00:00:00 EDT 2018

Microbial, metabolomic, and immunologic dynamics in a relapsing genetic mouse model of colitis induced by T-synthase deficiency.

Gut microbes

Jacobs JP,Lin L,Goudarzi M,Ruegger P,McGovern DP,Fornace AJ,Borneman J,Xia L,Braun J

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Mon Jan 02 00:00:00 EST 2017

Cutting Edge: Increased Autoimmunity Risk in Glycogen Storage Disease Type 1b Is Associated with a Reduced Engagement of Glycolysis in T Cells and an Impaired Regulatory T Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Melis D,Carbone F,Minopoli G,La Rocca C,Perna F,De Rosa V,Galgani M,Andria G,Parenti G,Matarese G

12477442 was used in western blot to examine the effect of glucose-6-phosphate transporter mutation on immune cell homeostasis and CD4+ T cell functions

Mon May 15 00:00:00 EDT 2017

Cutting Edge: Increased Autoimmunity Risk in Glycogen Storage Disease Type 1b Is Associated with a Reduced Engagement of Glycolysis in T Cells and an Impaired Regulatory T Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Melis D,Carbone F,Minopoli G,La Rocca C,Perna F,De Rosa V,Galgani M,Andria G,Parenti G,Matarese G

12477442 was used in western blot to examine the effect of glucose-6-phosphate transporter mutation on immune cell homeostasis and CD4+ T cell functions

Mon May 15 00:00:00 EDT 2017

Characteristics of CD4⁺CD25⁺Foxp3⁺ regulatory T cells in patients with multiple organ dysfunction syndrome.

Experimental and therapeutic medicine

Zang X,Jiang Y,Li X,DU Y,Niu J

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants.

Nature immunology

De Rosa V,Galgani M,Porcellini A,Colamatteo A,Santopaolo M,Zuchegna C,Romano A,De Simone S,Procaccini C,La Rocca C,Carrieri PB,Maniscalco GT,Salvetti M,Buscarinu MC,Franzese A,Mozzillo E,La Cava A,Matarese G

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus.

Arthritis & rheumatology (Hoboken, N.J.)

Wang D,Feng X,Lu L,Konkel JE,Zhang H,Chen Z,Li X,Gao X,Lu L,Shi S,Chen W,Sun L

12-4774 was used in Functional assays to highlight a previously unrecognised CD8+ T cell/IFNγ/IDO axis that mediates the therapeutic effects of allogeneic MSCs in lupus patients.

Fri Aug 01 00:00:00 EDT 2014

Elimination of IL-10-inducing T-helper epitopes from an IGFBP-2 vaccine ensures potent antitumor activity.

Cancer research

Cecil DL,Holt GE,Park KH,Gad E,Rastetter L,Childs J,Higgins D,Disis ML

12-4774 was used in Flow cytometry/Cell sorting to investigate the deletion of immunosuppressive epitopes from an IGFBP-2 cancer vaccine as a novel strategy to increase antitumor activity.

Thu May 15 00:00:00 EDT 2014

The effect of citrus peel extracts on cytokines levels and T regulatory cells in acute liver injury.

BioMed research international

Pantsulaia Ia,Iobadze M,Pantsulaia N,Chikovani T

12-4774 was used in Flow cytometry/Cell sorting to indicate that citrus peel extract in vivo could ameliorate Con A induced hepatitis.

Mon May 11 00:00:00 EDT 2015

CD40 deficiency in mice exacerbates obesity-induced adipose tissue inflammation, hepatic steatosis, and insulin resistance.

American journal of physiology. Endocrinology and metabolism

Guo CA,Kogan S,Amano SU,Wang M,Dagdeviren S,Friedline RH,Aouadi M,Kim JK,Czech MP

Published figure using FOXP3 monoclonal antibody (Product # 12-4774-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

生物信息学

蛋白别名: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy

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基因别名: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; RGD1562112; scurfin; sf; XPID

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UniProt ID: (Human) Q9BZS1, (Mouse) Q99JB6

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Entrez Gene ID: (Human) 50943, (Mouse) 20371, (Rat) 317382

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功能