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Invitrogen
MHC Class II (I-A) Monoclonal Antibody (NIMR-4), PE, eBioscience™
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FIGURE: 1 / 7
MHC Class II (I-A) Antibody (12-5322-81) in Flow
Nature communications 2018 -
Fig. 9 Physical interaction between TMPs and the DC surface is critical for TMP-mediated DC activation. a Schematic diagram of the transwell system (left). After co-incubation for 2 h, DCs were acid washed and evaluated for TCRbeta and V5G transfer (upper panel), followed continued incubation for 24 h. The expression of MHC class II, CD80, CD86, and CD40 on DCs (CD11c + ) was analysed by flow cytometry an... View More
Product Details
12-5322-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
C. elegans,
Mouse
Host/Isotype
Rat
/ IgG2b
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
NIMR-4
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells. MHC Class II molecules are important for the selection of the TCR repertoire during the processes of positive and negative selection. MHC Class II expression is required for the positive selection of CD4+ T cells.
Applications Reported: This NIMR-4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This NIMR-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: H-2 class II histocompatibility antigen, A beta chain; histocompatibility 2, class II antigen A, beta 1; major histocompatibility complex class II beta chain; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1
Gene Aliases: Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1
UniProt ID: (Mouse) P14483
Entrez Gene ID: (Mouse) 14961
B cell affinity maturation
immune system process
immunoglobulin production involved in immunoglobulin mediated immune response
humoral immune response mediated by circulating immunoglobulin
antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
positive regulation of antigen processing and presentation
positive regulation of T-helper 1 type immune response
immune response
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
negative regulation of T cell proliferation
positive regulation of alpha-beta T cell activation
antigen processing and presentation of peptide antigen
cellular response to interferon-gamma
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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