Invitrogen

KLRG1 Monoclonal Antibody (2F1), PE, eBioscience™

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KLRG1 Antibody (12-5893-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.125 µg of Golden Syrian Hamster IgG Isotype Control (Product # 12-4914-81) (left) or 0.125 µg of Anti-Mouse KLRG1 PE (right). Total viable cells were used for analysis.

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产品信息

12-5893-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看25篇已发表文章 25篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2F1

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10596642

产品详细信息

Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.

Applications Reported: This 2F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Murine cytomegalovirus downregulates ERAAP and induces an unconventional T cell response to self.

Cell reports

Geiger KM,Manoharan M,Coombs R,Arana K,Park CS,Lee AY,Shastri N,Robey EA,Coscoy L

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Tue Apr 25 00:00:00 EDT 2023

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Maturation and specialization of group 2 innate lymphoid cells through the lung-gut axis.

Nature communications

Zhao M,Shao F,Yu D,Zhang J,Liu Z,Ma J,Xia P,Wang S

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Fri Dec 09 00:00:00 EST 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Combination blockade of KLRG1 and PD-1 promotes immune control of local and disseminated cancers.

Oncoimmunology

Tata A,Dodard G,Fugère C,Leget C,Ors M,Rossi B,Vivier E,Brossay L

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Tue Jun 15 00:00:00 EDT 2021

Hedgehog interacting protein-expressing lung fibroblasts suppress lymphocytic inflammation in mice.

JCI insight

Yun JH,Lee C,Liu T,Liu S,Kim EY,Xu S,Curtis JL,Pinello L,Bowler RP,Silverman EK,Hersh CP,Zhou X

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses.

The Journal of experimental medicine

Chen L,Christian DA,Kochanowsky JA,Phan AT,Clark JT,Wang S,Berry C,Oh J,Chen X,Roos DS,Beiting DP,Koshy AA,Hunter CA

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

Characterization of Transcriptional Regulatory Networks that Promote and Restrict Identities and Functions of Intestinal Innate Lymphoid Cells.

Immunity

Pokrovskii M,Hall JA,Ochayon DE,Yi R,Chaimowitz NS,Seelamneni H,Carriero N,Watters A,Waggoner SN,Littman DR,Bonneau R,Miraldi ER

12-5893 was used in Flow cytometry/Cell sorting to study regulatory interactions between transcription factors and genes within intestinal type 1, 2, and 3 innate lymphoid cells subsets.

Tue Jul 16 00:00:00 EDT 2019

Natural killer cell monitoring in cutaneous melanoma - new dynamic biomarker.

Oncology letters

Isvoranu G,Surcel M,Huică RI,Munteanu AN,Pîrvu IR,Ciotaru D,Constantin C,Bratu O,Neagu M,Ursaciuc C

12-5893 was used in Flow cytometry/Cell sorting to show natural killer cells, in the spleen of mice carrying a melanoma, are reduced and that their phenotype changed.

Wed May 01 00:00:00 EDT 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.

Frontiers in immunology

Knolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Repeated Antigen Exposure Extends the Durability of Influenza-Specific Lung-Resident Memory CD8⁺ T Cells and Heterosubtypic Immunity.

Cell reports

Van Braeckel-Budimir N,Varga SM,Badovinac VP,Harty JT

12-5893 was used in Flow cytometry/Cell sorting to demonstrate that, relative to single virus exposure, repeated antigen exposure dramatically alters the dynamics of influenza-specific lung-resident primary memory CD8+ T cell populations.

Tue Sep 25 00:00:00 EDT 2018

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

Thu Sep 21 00:00:00 EDT 2017

Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.

Oncoimmunology

Huang RY,Francois A,McGray AR,Miliotto A,Odunsi K

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

CD4(+) T-cell dependence of primary CD8(+) T-cell response against vaccinia virus depends upon route of infection and viral dose.

Cellular & molecular immunology

Hu Z,Molloy MJ,Usherwood EJ

12-5893 was used in Flow cytometry/Cell sorting to investigate the role of of CD4(+) T-cell help in CD8(+) T-cell responses against viral infections.

Fri Jan 01 00:00:00 EST 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

CD70 and IFN-1 selectively induce eomesodermin or T-bet and synergize to promote CD8+ T-cell responses.

European journal of immunology

Dong H,Franklin NA,Ritchea SB,Yagita H,Glennie MJ,Bullock TN

12-5893 was used in Flow cytometry/Cell sorting to identify complex interactions between signal 3 and costimulatory pathways, and identify opportunities to influence the differentiation of CD8(+) T-cell responses.

Tue Dec 01 00:00:00 EST 2015

Mast cells expedite control of pulmonary murine cytomegalovirus infection by enhancing the recruitment of protective CD8 T cells to the lungs.

PLoS pathogens

Ebert S,Becker M,Lemmermann NA,Büttner JK,Michel A,Taube C,Podlech J,Böhm V,Freitag K,Thomas D,Holtappels R,Reddehase MJ,Stassen M

12-5893 was used in Flow cytometry/Cell sorting to explore the role of mast cells in the recruitment of protective CD8 T cells to the lungs during systemic infection.

Tue Apr 01 00:00:00 EDT 2014

MHC-dependent inhibition of uterine NK cells impedes fetal growth and decidual vascular remodelling.

Nature communications

Kieckbusch J,Gaynor LM,Moffett A,Colucci F

Published figure using KLRG1 monoclonal antibody (Product # 12-5893-82) in Flow Cytometry

Fri Feb 28 00:00:00 EST 2014

Markers of nonselective and specific NK cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Fogel LA,Sun MM,Geurs TL,Carayannopoulos LN,French AR

12-5893 was used in Flow cytometry/Cell sorting to demonstrate that a combination of Sca-1, CD27, and KLRG1 can distinguish NK cells based on their mechanism of activation.

Sat Jun 15 00:00:00 EDT 2013

Killer cell lectin-like receptor G1 deficiency significantly enhances survival after Mycobacterium tuberculosis infection.

Infection and immunity

Cyktor JC,Carruthers B,Stromberg P,Flaño E,Pircher H,Turner J

12-5893 was used in Flow cytometry/Cell sorting to investigate the requirement of the T cell differentiation marker KLRG1 during M. tuberculosis infection, showing that deficiency significantly enhances survival.

Mon Apr 01 00:00:00 EDT 2013

Antigen-presenting cells of haematopoietic origin prime cytomegalovirus-specific CD8 T-cells but are not sufficient for driving memory inflation during viral latency.

The Journal of general virology

Seckert CK,Schader SI,Ebert S,Thomas D,Freitag K,Renzaho A,Podlech J,Reddehase MJ,Holtappels R

12-5893 was used in Flow cytometry/Cell sorting to investigate the role of bone marrow-derived professional antigen-presenting cells in CD8 T-cell priming and memory to murine cytomegalovirus.