Invitrogen

PLZF Monoclonal Antibody (Mags.21F7), PE, eBioscience™

图: 1 / 4

PLZF Antibody (12-9320-82) in Flow

TOP: C57Bl/6 thymocytes were stained with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and Anti-Mouse TCR beta eFluor 450 (Product # 48-5961-82) followed by intracellular staining with 0.25 µg Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Mouse PLZF PE (right) using the Foxp3 Staining Buffer Set and protocol (Product # 00-5523-00). TCR beta+ cells in the lymphocyte gate were used for analysis. BOTTOM: C57Bl/6 thymocytes were stained with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and Anti-Mouse TCR beta eFluor 450 (... View More

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产品信息

12-9320-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看13篇已发表文章 13篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

Mags.21F7

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11148934

产品详细信息

Description: This Mags.21F7 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, and growth suppression. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor.

Applications Reported: This Mags.21F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Mags.21F7 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse lymph node cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Transcriptional profiling of human Vδ1 T cells reveals a pathogen-driven adaptive differentiation program.

Cell reports

McMurray JL,von Borstel A,Taher TE,Syrimi E,Taylor GS,Sharif M,Rossjohn J,Remmerswaal EBM,Bemelman FJ,Vieira Braga FA,Chen X,Teichmann SA,Mohammed F,Berry AA,Lyke KE,Williamson KC,Stubbington MJT,Davey MS,Willcox CR,Willcox BE

12-9320-82 was used in Flow Cytometry to show that adult Vδ1+ T cells segregate into TCF7+LEF1+Granzyme Bneg (Tnaive) or T-bet+Eomes+BLIMP-1+Granzyme B+.

Tue May 24 00:00:00 EDT 2022

Loss of Ubiquitin Carboxy-Terminal Hydrolase L1 Impairs Long-Term Differentiation Competence and Metabolic Regulation in Murine Spermatogonial Stem Cells.

Cells

Alpaugh WF,Voigt AL,Dardari R,Su L,Al Khatib I,Shin W,Goldsmith TM,Coyle KM,Tang LA,Shutt TE,Klein C,Biernaskie J,Dobrinski I

Published figure using PLZF monoclonal antibody (Product # 12-9320-82) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

Published figure using PLZF monoclonal antibody (Product # 12-9320-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

Commensal Microbiota Promote Lung Cancer Development via γδ T Cells.

Cell

Jin C,Lagoudas GK,Zhao C,Bullman S,Bhutkar A,Hu B,Ameh S,Sandel D,Liang XS,Mazzilli S,Whary MT,Meyerson M,Germain R,Blainey PC,Fox JG,Jacks T

12-9320 was used in Flow cytometry/Cell sorting to link local microbiota-immune crosstalk to lung tumor development and thereby define key cellular and molecular mediators that may serve as effective targets in lung cancer intervention.

Thu Feb 21 00:00:00 EST 2019

Transnuclear mice reveal Peyer's patch iNKT cells that regulate B-cell class switching to IgG1.

The EMBO journal

Clancy-Thompson E,Chen GZ,LaMarche NM,Ali LR,Jeong HJ,Crowley SJ,Boelaars K,Brenner MB,Lynch L,Dougan SK

Published figure using PLZF monoclonal antibody (Product # 12-9320-82) in Flow Cytometry

Mon Jul 15 00:00:00 EDT 2019

In Vitro Cytotoxicity of Folate-Silica-Gold Nanorods on Mouse Acute Lymphoblastic Leukemia and Spermatogonial Cells.

Cell journal

Eslahi N,Shakeri-Zadeh A,Ashtari K,Pirhajati-Mahabadi V,Tohidi Moghadam T,Shabani R,Kamrava K,Madjd Z,Maki C,Asgari HR,Koruji M

Published figure using PLZF monoclonal antibody (Product # 12-9320-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Invariant natural killer T-cell development and function with loss of microRNA-155.

Immunology

Frias AB,Buechel HM,Neupane A,D'Cruz LM

12-9320 was used in Flow cytometry/Cell sorting to examine the role of microRNA-155 in the development and function of invariant natural killer T cells.

Thu Feb 01 00:00:00 EST 2018

Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.

Cell

Lim AI,Li Y,Lopez-Lastra S,Stadhouders R,Paul F,Casrouge A,Serafini N,Puel A,Bustamante J,Surace L,Masse-Ranson G,David E,Strick-Marchand H,Le Bourhis L,Cocchi R,Topazio D,Graziano P,Muscarella LA,Rogge L,Norel X,Sallenave JM,Allez M,Graf T,Hendriks RW,Casanova JL,Amit I,Yssel H,Di Santo JP

12-9320 was used in Flow cytometry/Cell sorting to identify circulating and tissue ILC precursors in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration.

Thu Mar 09 00:00:00 EST 2017

MR1-restricted mucosal-associated invariant T (MAIT) cells respond to mycobacterial vaccination and infection in nonhuman primates.

Mucosal immunology

Greene JM,Dash P,Roy S,McMurtrey C,Awad W,Reed JS,Hammond KB,Abdulhaqq S,Wu HL,Burwitz BJ,Roth BF,Morrow DW,Ford JC,Xu G,Bae JY,Crank H,Legasse AW,Dang TH,Greenaway HY,Kurniawan M,Gold MC,Harriff MJ,Lewinsohn DA,Park BS,Axthelm MK,Stanton JJ,Hansen SG,Picker LJ,Venturi V,Hildebrand W,Thomas PG,Lewinsohn DM,Adams EJ,Sacha JB

12-9320 was used in Flow cytometry/Cell sorting to investigate the response of MR1 restricted mucosal-associated invariant T cells to mycobacterial vaccination and infection in nonhuman primates.

Mon May 01 00:00:00 EDT 2017

Reactive Oxygen Species Regulate the Inflammatory Function of NKT Cells through Promyelocytic Leukemia Zinc Finger.

Journal of immunology (Baltimore, Md. : 1950)

Kim YH,Kumar A,Chang CH,Pyaram K

12-9320 was used in Flow cytometry/Cell sorting to investigate the role of reactive oxygen species in natural killer T cell function.

Wed Nov 15 00:00:00 EST 2017

The Hypothesis of the Human iNKT/Innate CD8(+) T-Cell Axis Applied to Cancer: Evidence for a Deficiency in Chronic Myeloid Leukemia.

Frontiers in immunology

Jacomet F,Cayssials E,Barbarin A,Desmier D,Basbous S,Lefèvre L,Levescot A,Robin A,Piccirilli N,Giraud C,Guilhot F,Roy L,Herbelin A,Gombert JM

Wed Nov 20 00:00:00 EST 2019

Id2 regulates hyporesponsive invariant natural killer T cells.

Immunology and cell biology

Stradner MH,Cheung KP,Lasorella A,Goldrath AW,D'Cruz LM

12-9320 was used in Flow cytometry/Cell sorting to propose that downregulation of Id2 expression is essential to induction of the anti-inflammatory state in iNKT cells.

Mon Aug 01 00:00:00 EDT 2016

E and Id proteins influence invariant NKT cell sublineage differentiation and proliferation.

Journal of immunology (Baltimore, Md. : 1950)

D'Cruz LM,Stradner MH,Yang CY,Goldrath AW

12-9320 was used in Flow cytometry/Cell sorting to investigate the mechanism by which E and Id proteins control the development of invariant NKT sublineages after positive selection.

Sat Mar 01 00:00:00 EST 2014

生物信息学

蛋白别名: Green's luxoid; luxoid; promyelocytic leukaemia zinc finger; Promyelocytic leukemia zinc finger protein; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein PLZF

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基因别名: AI467657; lu; PLZF; ZBTB16; Zfp145; ZNF145

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UniProt ID: (Human) Q05516

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Entrez Gene ID: (Human) 7704, (Mouse) 235320

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功能