Invitrogen

Phospho-mTOR (Ser2448) Monoclonal Antibody (MRRBY), PE, eBioscience™

Phospho-mTOR (Ser2448) Antibody in Flow Cytometry (Flow)

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Phospho-mTOR (Ser2448) Antibody (12-9718-42) in Flow

TOP: Normal human peripheral blood cells were unstimulated (left) or stimulated with Anti-Human CD3 and CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81) for 48 hours (right). The cells were then intracellularly stained with Anti-Human CD3 FITC (Product # 11-0036-42) and Anti-Human/Mouse phospho-mTOR (S2448) PE using the Intracellular Fixation & Permeabilization Buffer Set (Pro... View More

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产品信息

12-9718-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

MRRBY

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572724

产品详细信息

Description: This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (also known as mTOR, FRAP, RAFT) when phosphorylated on S2448. mTOR is a serine/threonine protein kinase that functions as an ATP and amino acid sensor as well as to balance nutrient availability with cell growth, proliferation, motility, survival, protein synthesis, and transcription. Activated mTOR increases production of enzymes necessary for glycolysis and controls the uptake of glucose and other nutrients. Increased glucose uptake and metabolism helps fulfill the energy needs for mTOR-driven cell growth and proliferation. When sufficient nutrients are available, mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor, 4E-BP1. mTOR is phosphorylated at S2448 via the PI3 kinase/Akt signaling pathway and is autophosphorylated at Ser2481. Due to its critical role in regulation of cell growth, survival, and metabolism, and because it is often abnormally regulated in tumors, mTOR is under investigation as a potential target for anti-cancer therapy.

Applications Reported: This MRRBY antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MRRBY antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Autophagy-dependent glutaminolysis drives superior IL21 production in HIV-1-specific CD4 T cells.

Autophagy

Loucif H,Dagenais-Lussier X,Avizonis D,Choinière L,Beji C,Cassin L,Routy JP,Fritz JH,Olagnier D,van Grevenynghe J

12-9718-42 was used in Flow cytometry/Cell sorting to investigate whether the beneficial role of a strong macroautophagy/autophagy, could also be used to ensure effective IL21 production as well.

Wed Jun 01 00:00:00 EDT 2022

Dysregulated PI3K Signaling in B Cells of CVID Patients.

Cells

Harder I,Münchhalfen M,Andrieux G,Boerries M,Grimbacher B,Eibel H,Maccari ME,Ehl S,Wienands J,Jellusova J,Warnatz K,Keller B

12-9718-42 was used in Flow Cytometry to investigate PI3K signaling and its targets as crucial regulators of survival, proliferation and metabolism by intracellular flow cytometry, imaging flow cytometry and RNAseq.

Fri Jan 28 00:00:00 EST 2022

Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity.

Nature communications

Yang W,Yu T,Huang X,Bilotta AJ,Xu L,Lu Y,Sun J,Pan F,Zhou J,Zhang W,Yao S,Maynard CL,Singh N,Dann SM,Liu Z,Cong Y

Published figure using Phospho-mTOR (Ser2448) monoclonal antibody (Product # 12-9718-42) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2020

PRMT5 Modulates Splicing for Genome Integrity and Preserves Proteostasis of Hematopoietic Stem Cells.

Cell reports

Tan DQ,Li Y,Yang C,Li J,Tan SH,Chin DWL,Nakamura-Ishizu A,Yang H,Suda T

12-9718 was used in Immunocytochemistry-immunoflourescence to establish the importance of cell-intrinsic protein arginine methyltransferase 5 activity in hematopoietic stem cells.

Tue Feb 26 00:00:00 EST 2019

Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells.

The Journal of clinical investigation

Alissafi T,Hatzioannou A,Mintzas K,Barouni RM,Banos A,Sormendi S,Polyzos A,Xilouri M,Wielockx B,Gogas H,Verginis P

12-9718 was used in Flow cytometry/Cell sorting to identify autophagy as a crucial pathway for myeloid-derived suppressor cell-mediated suppression of antitumour immunity.

Fri Aug 31 00:00:00 EDT 2018

mTOR-Dependent Oxidative Stress Regulates oxLDL-Induced Trained Innate Immunity in Human Monocytes.

Frontiers in immunology

Sohrabi Y,Lagache SMM,Schnack L,Godfrey R,Kahles F,Bruemmer D,Waltenberger J,Findeisen HM

Published figure using Phospho-mTOR (Ser2448) monoclonal antibody (Product # 12-9718-42) in Flow Cytometry

Mon Oct 14 00:00:00 EDT 2019

Store-Operated Ca²⁺ Entry Controls Clonal Expansion of T Cells through Metabolic Reprogramming.

Immunity

Vaeth M,Maus M,Klein-Hessling S,Freinkman E,Yang J,Eckstein M,Cameron S,Turvey SE,Serfling E,Berberich-Siebelt F,Possemato R,Feske S

Published figure using Phospho-mTOR (Ser2448) monoclonal antibody (Product # 12-9718-42) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Mitochondrial activation chemicals synergize with surface receptor PD-1 blockade for T cell-dependent antitumor activity.

Proceedings of the National Academy of Sciences of the United States of America

Chamoto K,Chowdhury PS,Kumar A,Sonomura K,Matsuda F,Fagarasan S,Honjo T

12971842 was used in flow cytometry to characterize mitochondrial changes in T cells due to PD-1 blockade therapy

Tue Jan 31 00:00:00 EST 2017

生物信息学

蛋白别名: angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR

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基因别名: 2610315D21Rik; AI327068; flat; FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS

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UniProt ID: (Human) P42345, (Mouse) Q9JLN9

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Entrez Gene ID: (Human) 2475, (Mouse) 56717

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