Invitrogen

CD284 (TLR4) Monoclonal Antibody (HTA125), PE, eBioscience™

CD284 (TLR4) Antibody in Flow Cytometry (Flow)

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CD284 (TLR4) Antibody (12-9917-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (open histogram) or Anti-Human CD284 (TLR4) PE (filled histogram). Cells in the monocyte gate were used for analysis.

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产品信息

12-9917-42

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (2 µg)/test

查看78篇已发表文章 78篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Dog, Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

HTA125

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1603311

产品详细信息

Description: The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (~100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors.

It is highly recommended that for optimal staining of TLR4, whole blood be stained using the lysed whole blood protocol (found in Best Protocols) rather than Ficoll-gradient prepared normal human peripheral blood cells. The use of a density gradient appears to reduce the staining intensity significantly.

Applications Reported: The HTA125 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HTA125 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (2.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.

Cell reports

Di Nardo A,Chang YL,Alimohammadi S,Masuda-Kuroki K,Wang Z,Sriram K,Insel PA

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Tue May 30 00:00:00 EDT 2023

Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture model.

Particle and fibre toxicology

Bredeck G,Dobner J,Stahlmecke B,Fomba KW,Herrmann H,Rossi A,Schins RPF

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Leptin and TGF-β1 Downregulate PREP1 Expression in Human Adipose-Derived Mesenchymal Stem Cells and Mature Adipocytes.

Frontiers in cell and developmental biology

Bruno A,Di Sano C,Simon HU,Chanez P,Patti AM,Di Vincenzo S,Dino P,D'Esposito V,Formisano P,Beguinot F,Pace E

12-9917-42 was used in Flow Cytometry to assess for the first time if leptin and TGF-β interfere with PREP1 expression in both ASCs and mature differentiated adipocytes.

Sat Jul 31 00:00:00 EDT 2021

Blockade of TLR2 and TLR4 Attenuates Inflammatory Response and Parasite Load in Cutaneous Leishmaniasis.

Frontiers in immunology

Carneiro PP,Dórea AS,Oliveira WN,Guimarães LH,Brodskyn C,Carvalho EM,Bacellar O

12-9917-42 was used in Flow Cytometry to show that reduction in oxidative burst, IL-1β, TNF and CXCL9 production were observed.

Mon Dec 13 00:00:00 EST 2021

Cathelicidin-mediated lipopolysaccharide signaling via intracellular TLR4 in colonic epithelial cells evokes CXCL8 production.

Gut microbes

Holani R,Babbar A,Blyth GAD,Lopes F,Jijon H,McKay DM,Hollenberg MD,Cobo ER

12-9917-42 was used in Flow Cytometry to investigate if cathelicidin has a unique pathogen-sensing role, facilitating LPS-mediated intestinal responses, including the production of CXCL8/CXCL1 that would contribute to an integrated tissue response to recruit neutrophils during colitis.

Mon Nov 09 00:00:00 EST 2020

Grindelia squarrosa Extract and Grindelic Acid Modulate Pro-inflammatory Functions of Respiratory Epithelium and Human Macrophages.

Frontiers in pharmacology

Gierlikowska B,Filipek A,Gierlikowski W,Kania D,Stefańska J,Demkow U,Kiss AK

12-9917-42 was used in Flow Cytometry to study whether successful treatment of cold-related diseases may arise from modulation of the pro-inflammatory functions of respiratory epithelium and human monocytes/macrophages.

Sat Feb 06 00:00:00 EST 2021

Grindelia squarrosa Extract and Grindelic Acid Modulate Pro-inflammatory Functions of Respiratory Epithelium and Human Macrophages.

Frontiers in pharmacology

Gierlikowska B,Filipek A,Gierlikowski W,Kania D,Stefańska J,Demkow U,Kiss AK

Sat Feb 06 00:00:00 EST 2021

Bacterial lipopolysaccharide and antimicrobial LL-37 enhance ICAM-1 expression and NF-κB p65 phosphorylation in senescent endothelial cells.

International journal of molecular medicine

Suzuki K,Ohkuma M,Nagaoka I

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Human lactoferrin attenuates the proinflammatory response of neonatal monocyte-derived macrophages.

Clinical and experimental immunology

Wisgrill L,Wessely I,Spittler A,Förster-Waldl E,Berger A,Sadeghi K

12-9917 was used in Flow cytometry/Cell sorting to investigate the immunomodulatory effects of human lactoferrin on differentiation and activation of monocyte-derived macrophages.

Fri Jun 01 00:00:00 EDT 2018

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Mon Jan 01 00:00:00 EST 2018

Immune Function in Critically Ill Dogs.

Journal of veterinary internal medicine

Hoffman D,Amorim J,DeClue A

12-9917 was used in Flow cytometry/Cell sorting to study how the immune response in critically injured dogs differs from that of healthy dogs.

Mon Jan 01 00:00:00 EST 2018

Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.

Oncology letters

Xiao T,Wu S,Yan C,Zhao C,Jin H,Yan N,Xu J,Wu Y,Li C,Shao Q,Xia S

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function.

Scientific reports

Hamon R,Tran HB,Roscioli E,Ween M,Jersmann H,Hodge S

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2018

Altered Toll-like receptor expression and function in HPV-associated oropharyngeal carcinoma.

Oncotarget

Tobouti PL,Bolt R,Radhakrishnan R,de Sousa SCOM,Hunter KD

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

A low dose lipid infusion is sufficient to induce insulin resistance and a pro-inflammatory response in human subjects.

PloS one

Liang H,Lum H,Alvarez A,Garduno-Garcia JJ,Daniel BJ,Musi N

12-9917 was used in Flow cytometry/Cell sorting to investigate whether an experimental elevation in plasma FFA in lean individuals would upregulate TLR4 and activate downstream pathways in circulating monocytes.

Fri Jul 27 00:00:00 EDT 2018

A low dose lipid infusion is sufficient to induce insulin resistance and a pro-inflammatory response in human subjects.

PloS one

Liang H,Lum H,Alvarez A,Garduno-Garcia JJ,Daniel BJ,Musi N

Fri Jul 27 00:00:00 EDT 2018

Multi-cellular human bronchial models exposed to diesel exhaust particles: assessment of inflammation, oxidative stress and macrophage polarization.

Particle and fibre toxicology

Ji J,Upadhyay S,Xiong X,Malmlöf M,Sandström T,Gerde P,Palmberg L

12-9917 was used in Flow cytometry/Cell sorting to identify interaction of primary bronchial epithelial cells with macrophages in response to diesel exhaust particles aid macrophage phenotypic alteration towards M2-subtypes.

Wed May 02 00:00:00 EDT 2018

Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein 180L Single-Nucleotide Polymorphism Is Associated With Susceptibility to Recurrent Pneumococcal Lower Respiratory Tract Infections in Children.

Frontiers in immunology

Siebert JN,Hamann L,Verolet CM,Gameiro C,Grillet S,Siegrist CA,Posfay-Barbe KM

12-9917 was used in Flow cytometry/Cell sorting to asses whether a defect somewhere along this toll-like receptor signally pathways increases susceptibility to recurrent pneumococcal low respiratory tract infections.

Mon Sep 16 00:00:00 EDT 2019

Innate inflammatory gene expression profiling in potential brain-dead donors: detailed investigation of the effect of common corticosteroid therapy.

Innate immunity

Gholamnezhadjafari R,Tajik N,Falak R,Aflatoonian R,Dehghan S,Rezaei A

12-9917 was used in Flow cytometry/Cell sorting to report that common methylprednisolone therapy in brain-dead organ donors did not adequately reduce systemic inflammation, which could be due to inadequate doses or inefficient impact on other inflammatory-inducing pathways.

Sat Jul 01 00:00:00 EDT 2017

The inflammatory response of neutrophils in an in vitro model that approximates the postcardiac arrest state.

Annals of surgical treatment and research

Cho YD,Park SJ,Choi SH,Yoon YH,Kim JY,Lee SW,Lim CS

12-9917 was used in Flow cytometry/Cell sorting to study the inflammatory response of human neutrophils in hypoxia-normoxia and hypoxia-hyperoxia environments.

Sun Oct 01 00:00:00 EDT 2017

Toll-like receptor 3 activation selectively reverses HIV latency in microglial cells.

Retrovirology

Alvarez-Carbonell D,Garcia-Mesa Y,Milne S,Das B,Dobrowolski C,Rojas R,Karn J

12991742 was used in flow cytometry to study the role to toll-like receptors and microglia on HIV disease

Mon Feb 06 00:00:00 EST 2017

Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses.

European journal of immunology

Cheng WC,van Asten SD,Burns LA,Evans HG,Walter GJ,Hashim A,Hughes FJ,Taams LS

12-9917 was used in Flow cytometry/Cell sorting to investigate the mechanism by which the periodontal pathogens promote a Th17/IL-17 response in vitro, showing that they activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses.

Thu Sep 01 00:00:00 EDT 2016

Early detection of neonatal group B streptococcus sepsis and the possible diagnostic utility of IL-6, IL-8, and CD11b in a human umbilical cord blood in vitro model.

Infection and drug resistance

Nakstad B,Sonerud T,Solevåg AL

12-9917 was used in Flow cytometry/Cell sorting to identify biomarkers with high sensitivity and specificity in an early stage of group B streptococcus infection.

Fri Jul 29 00:00:00 EDT 2016

Early detection of neonatal group B streptococcus sepsis and the possible diagnostic utility of IL-6, IL-8, and CD11b in a human umbilical cord blood in vitro model.

Infection and drug resistance

Nakstad B,Sonerud T,Solevåg AL

12-9917 was used in Flow cytometry/Cell sorting to identify biomarkers with high sensitivity and specificity in an early stage of group B streptococcus infection.

Fri Jul 29 00:00:00 EDT 2016

TLR4/ROS/miRNA-21 pathway underlies lipopolysaccharide instructed primary tumor outgrowth in lung cancer patients.

Oncotarget

Zhang X,Wang C,Shan S,Liu X,Jiang Z,Ren T

12-9917 was used in Flow cytometry/Cell sorting to study the molecular mechanisms behind primary human lung cancer outgrowth in response to LPS.

Tue Jul 05 00:00:00 EDT 2016

Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk.

PLoS pathogens

El-Awady AR,Miles B,Scisci E,Kurago ZB,Palani CD,Arce RM,Waller JL,Genco CA,Slocum C,Manning M,Schoenlein PV,Cutler CW

12-9917 was used in Flow cytometry/Cell sorting to reveal that selective engagement of DC-SIGN by Mfa-1+P. gingivalis results in intracellular persistence in myeloid DCs.

Sun Feb 01 00:00:00 EST 2015

In vivo pre-activation of monocytes in patients with axial spondyloarthritis.

Arthritis research & therapy

Conrad K,Wu P,Sieper J,Syrbe U

12-9917 was used in Flow cytometry/Cell sorting to demonstrate in vivo pre-activation of monocytes in axial spondyloarthritis patients indicated by enhanced cytokine production by monocytes.

Thu Jul 16 00:00:00 EDT 2015

TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery.

PloS one

Avlas O,Bragg A,Fuks A,Nicholson JD,Farkash A,Porat E,Aravot D,Levy-Drummer RS,Cohen C,Shainberg A,Arad M,Hochhauser E

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2016

Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors.

Toxins

Latorre D,Pulvirenti N,Covino DA,Varano B,Purificato C,Rainaldi G,Gauzzi MC,Fantuzzi L,Conti L,Donninelli G,Del Cornò M,Sabbatucci M,Gessani S,Puddu P

12-9917 was used in Flow cytometry/Cell sorting to investigate the effect of lactoferrin on monocyte-derived dendritic cells.

Thu Dec 17 00:00:00 EST 2015

The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane.

Science signaling

Liaunardy-Jopeace A,Bryant CE,Gay NJ

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Tue Jul 29 00:00:00 EDT 2014

S100A4 and uric acid promote mesenchymal stromal cell induction of IL-10+/IDO+ lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Eisenbacher JL,Schrezenmeier H,Jahrsdörfer B,Kaltenmeier C,Rojewski MT,Yildiz T,Beyer T,Erle A,Wiegmann DS,Grassl S,Hang R,Körper S,Wiesneth M,Lotze MT,Lotfi R

12-9917 was used in Flow cytometry/Cell sorting to assess the role played by the damage-associated molecular patterns, S100A4 and U4, in mesenchymal stromal cell-associated immunoregulation.

Sun Jun 15 00:00:00 EDT 2014

Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Balan S,Ollion V,Colletti N,Chelbi R,Montanana-Sanchis F,Liu H,Vu Manh TP,Sanchez C,Savoret J,Perrot I,Doffin AC,Fossum E,Bechlian D,Chabannon C,Bogen B,Asselin-Paturel C,Shaw M,Soos T,Caux C,Valladeau-Guilemond J,Dalod M

12-9917 was used in Flow cytometry/Cell sorting to validate a novel tissue culture protocol to generate both XCR1(+) and XCR1(-) human dendritic cells from CD34(+) progenitors.

Fri Aug 15 00:00:00 EDT 2014

Interactions between alveolar epithelial cells and neutrophils under pro-inflammatory conditions.

European clinical respiratory journal

von Schéele I,Larsson K,Palmberg L

12-9917 was used in Flow cytometry/Cell sorting to investigate communication between cells constituting the first line of defence under infection from chronic obstructive pulmonary disease.

Wed Nov 11 00:00:00 EST 2015

The effect of hypertonic saline on mRNA of proinflammatory cytokines in lipopolysaccharide-stimulated polymorphonuclear cells.

Current therapeutic research, clinical and experimental

Choi SH,Yoon YH,Kim JY,Moon SW,Cho YD,Yeom JW

12-9917 was used in Flow cytometry/Cell sorting to investigate the immunomodulatory effect of hypertonic saline on polymorphonuclear cells.

Mon Dec 01 00:00:00 EST 2014

Porphyromonas Gingivalis and E-coli induce different cytokine production patterns in pregnant women.

PloS one

Faas MM,Kunnen A,Dekker DC,Harmsen HJ,Aarnoudse JG,Abbas F,De Vos P,Van Pampus MG

12-9917 was used in Flow cytometry/Cell sorting to demonstrate that the different responses of pregnant women to Porphyromonas Gingivalis and E-coli may result from variations in cytokine production.

Tue Nov 04 00:00:00 EST 2014

Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity.

PloS one

Johnston JB,Nickerson JG,Daroszewski J,Mogg TJ,Burton GW

12-9917 was used in Flow cytometry/Cell sorting to study carotenoid activity via biologically active polymers from spontaneous carotenoid oxidation.

Thu Jun 25 00:00:00 EDT 2015

ATF4 is directly recruited by TLR4 signaling and positively regulates TLR4-trigged cytokine production in human monocytes.

Cellular & molecular immunology

Zhang C,Bai N,Chang A,Zhang Z,Yin J,Shen W,Tian Y,Xiang R,Liu C

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Leptin up-regulates TLR2 in human monocytes.

Journal of leukocyte biology

Jaedicke KM,Roythorne A,Padget K,Todryk S,Preshaw PM,Taylor JJ

12-9917 was used in Flow cytometry/Cell sorting to elucidate the effect of leptin on PRRs.

Mon Apr 01 00:00:00 EDT 2013

Identification of lineage relationships and novel markers of blood and skin human dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Harman AN,Bye CR,Nasr N,Sandgren KJ,Kim M,Mercier SK,Botting RA,Lewin SR,Cunningham AL,Cameron PU

12-9917 was used in Flow cytometry/Cell sorting to study the lineage relationships between blood and tissue dendritic cells, providing novel markers of blood and skin human dendritic cells.

Tue Jan 01 00:00:00 EST 2013

Development of monocyte Toll-like receptor 2 and Toll-like receptor 4 in preterm newborns during the first few months of life.

Pediatric research

Shen CM,Lin SC,Niu DM,Kou YR

12-9917 was used in Flow Cytometry to study the development of Toll-like receptor 2 and Toll-like receptor 4 at birth in preterm newborns and during the first few months of life.

Wed May 01 00:00:00 EDT 2013

Body fluid exosomes promote secretion of inflammatory cytokines in monocytic cells via Toll-like receptor signaling.

The Journal of biological chemistry

Bretz NP,Ridinger J,Rupp AK,Rimbach K,Keller S,Rupp C,Marmé F,Umansky L,Umansky V,Eigenbrod T,Sammar M,Altevogt P

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Fri Dec 20 00:00:00 EST 2013

Body fluid exosomes promote secretion of inflammatory cytokines in monocytic cells via Toll-like receptor signaling.

The Journal of biological chemistry

Bretz NP,Ridinger J,Rupp AK,Rimbach K,Keller S,Rupp C,Marmé F,Umansky L,Umansky V,Eigenbrod T,Sammar M,Altevogt P

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Fri Dec 20 00:00:00 EST 2013

Surface expression and genotypes of Toll-like receptors 2 and 4 in patients with juvenile idiopathic arthritis and systemic lupus erythematosus.

Pediatric rheumatology online journal

Kirchner M,Sonnenschein A,Schoofs S,Schmidtke P,Umlauf VN,Mannhardt-Laakmann W

12-9917 was used in Flow cytometry/Cell sorting to investigate the role of Toll-like receptors in juvenile idiopathic arthritis and systemic lupus erythematosus.

Tue Mar 05 00:00:00 EST 2013

Vitamin D deficiency impacts on expression of toll-like receptor-2 and cytokine profile: a pilot study.

Journal of translational medicine

Ojaimi S,Skinner NA,Strauss BJ,Sundararajan V,Woolley I,Visvanathan K

12-9917 was used in Flow cytometry/Cell sorting to add evidence to the important role of vitamin D in innate immunity, possibly helping to reduce inflammation.

Mon Jul 22 00:00:00 EDT 2013

Monocytes from tuberculosis patients that exhibit cleaved caspase 9 and denaturalized cytochrome c are more susceptible to death mediated by Toll-like receptor 2.

Immunology

Chávez-Galán L,Sada-Ovalle I,Baez-Saldaña R,Chávez R,Lascurain R

12-9917 was used in Flow cytometry/Cell sorting to study the increased percentage of circulating monocytes in patients with tuberculosis in comparison to healthy controls.

Sun Apr 01 00:00:00 EDT 2012

Monocyte subpopulations from pre-eclamptic patients are abnormally skewed and exhibit exaggerated responses to Toll-like receptor ligands.

PloS one

Al-ofi E,Coffelt SB,Anumba DO

12-9917 was used in Flow cytometry/Cell sorting to analyse monocytic subpopulations in pre-eclampsia patients and normal pregnant controls.

Thu Jan 10 00:00:00 EST 2013

Monocyte-derived macrophages do not explain susceptibility to pulmonary non-tuberculous mycobacterial disease.

Clinical & translational immunology

de Jong E,Lim A,Waterer G,Price P

12-9917 was used in Flow cytometry/Cell sorting to elucidate whether Th1 cytokine production or host macrophage/monocyte responses were impaired in a subset of non-tuberculous mycobacteria-infected patients.

Thu Nov 01 00:00:00 EDT 2012

Neutrophil paralysis in Plasmodium vivax malaria.

PLoS neglected tropical diseases

Leoratti FM,Trevelin SC,Cunha FQ,Rocha BC,Costa PA,Gravina HD,Tada MS,Pereira DB,Golenbock DT,Antonelli LR,Gazzinelli RT

12-9917 was used in Flow cytometry/Cell sorting to investigate the activation and function of neutrophils during acute episodes of Plasmodium vivax malaria.

Fri Sep 28 00:00:00 EDT 2012

Hydroxychloroquine drastically reduces immune activation in HIV-infected, antiretroviral therapy-treated immunologic nonresponders.

Blood

Piconi S,Parisotto S,Rizzardini G,Passerini S,Terzi R,Argenteri B,Meraviglia P,Capetti A,Biasin M,Trabattoni D,Clerici M

12-9917 was used in Flow cytometry/Cell sorting to investigate the effect of hydroxychloroquine on immune activation and CD4+ T cell number in HIV-infected, antiretroviral therapy-treated immunologic nonresponders.

Thu Sep 22 00:00:00 EDT 2011

1,25-dihydroxyvitamin D3 modulates cytokine production induced by Candida albicans: impact of seasonal variation of immune responses.

The Journal of infectious diseases

Khoo AL,Chai LY,Koenen HJ,Kullberg BJ,Joosten I,van der Ven AJ,Netea MG

12-9917 was used in Flow cytometry/Cell sorting to show how vitamin D(3) skews Candida albicans induced cytokine responses towards an anti-inflammatory profile due to suppressed TLR2/4, Dectin-1 and MR transcription.

Sat Jan 01 00:00:00 EST 2011

Toll-like receptor expression in smokers with and without COPD.

Respiratory medicine

von Scheele I,Larsson K,Dahlén B,Billing B,Skedinger M,Lantz AS,Palmberg L

12-9917 was used in Flow cytometry/Cell sorting to study Toll-like receptor expression in smokers with and without chronic obstructive pulmonary disease.

Mon Aug 01 00:00:00 EDT 2011

Cigarette smoke induces the release of CXCL-8 from human bronchial epithelial cells via TLRs and induction of the inflammasome.

Biochimica et biophysica acta

Mortaz E,Henricks PA,Kraneveld AD,Givi ME,Garssen J,Folkerts G

12-9917 was used in Flow cytometry/Cell sorting to demonstrate that cigarette smoke releases CXCL-8 from HBE-14o cells via TLR4 and TLR9 and inflammasome activation.

Thu Sep 01 00:00:00 EDT 2011

The mechanism of excessive intestinal inflammation in necrotizing enterocolitis: an immature innate immune response.

PloS one

Nanthakumar N,Meng D,Goldstein AM,Zhu W,Lu L,Uauy R,Llanos A,Claud EC,Walker WA

12-9917 was used in Flow cytometry/Cell sorting to investigate if pathogenesis of necrotizing enterocolitis (NEC) associated with the excessive inflammatory IL-8 response, is related to an immature expression of innate immune response genes.

Mon Mar 21 00:00:00 EDT 2011

Diesel exhaust particles override natural injury-limiting pathways in the lung.

American journal of physiology. Lung cellular and molecular physiology

Chaudhuri N,Paiva C,Donaldson K,Duffin R,Parker LC,Sabroe I

12-9917 was used in Flow cytometry/Cell sorting to study a model of inflammation where diesel exhaust particles can alter the nature of the inflammatory milieu induced by TLR agonists.

Sun Aug 01 00:00:00 EDT 2010

Dendritic cells express hematopoietic prostaglandin D synthase and function as a source of prostaglandin D2 in the skin.

The American journal of pathology

Shimura C,Satoh T,Igawa K,Aritake K,Urade Y,Nakamura M,Yokozeki H

12-9917 was used in Flow cytometry/Cell sorting to determine that dendritic cells may mediate or regulate skin inflammation by releasing PGD2 in response to various stimuli.

Fri Jan 01 00:00:00 EST 2010

Human neonatal peripheral blood leukocytes demonstrate pathogen-specific coordinate expression of TLR2, TLR4/MD2, and MyD88 during bacterial infection in vivo.

Pediatric research

Zhang JP,Yang Y,Levy O,Chen C

12-9917 was used in Flow cytometry/Cell sorting to suggest key roles for innate immune pathways in neonatal responses to G+ and G- bacterial infection.

Wed Dec 01 00:00:00 EST 2010

Molecular pathways mediating differential responses to lipopolysaccharide between human and baboon arterial endothelial cells.

Clinical and experimental pharmacology & physiology

Shi Q,Cox LA,Glenn J,Tejero ME,Hondara V,Vandeberg JL,Wang XL

12-9917 was used in Flow cytometry/Cell sorting to indicate that an altered TLR4 system may be responsible for the resistance of baboon endothelial cells to LPS.

Mon Feb 01 00:00:00 EST 2010

Interleukin-25 inhibits interleukin-12 production and Th1 cell-driven inflammation in the gut.

Gastroenterology

Caruso R,Sarra M,Stolfi C,Rizzo A,Fina D,Fantini MC,Pallone F,MacDonald TT,Monteleone G

12-9917 was used in Flow cytometry/Cell sorting to demonstrate that IL-25 inhibits CD14+ cell-derived cytokines and experimental colitis, so could be a treatment for Crohn's disease and ulcerative colitis.

Mon Jun 01 00:00:00 EDT 2009

Fenoterol, a beta(2)-adrenoceptor agonist, inhibits LPS-induced membrane-bound CD14, TLR4/CD14 complex, and inflammatory cytokines production through beta-arrestin-2 in THP-1 cell line.

Acta pharmacologica Sinica

Wang W,Xu M,Zhang YY,He B

12-9917 was used in Flow cytometry/Cell sorting to investigate the molecular mechanism and signalling pathway by which fenoterol produces anti-inflammatory effects.

Sun Nov 01 00:00:00 EDT 2009

Candesartan inhibits Toll-like receptor expression and activity both in vitro and in vivo.

Atherosclerosis

Dasu MR,Riosvelasco AC,Jialal I

Published figure using CD284 (TLR4) monoclonal antibody (Product # 12-9917-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2009

Targeting of DC-SIGN on human dendritic cells by minor fimbriated Porphyromonas gingivalis strains elicits a distinct effector T cell response.

Journal of immunology (Baltimore, Md. : 1950)

Zeituni AE,Jotwani R,Carrion J,Cutler CW

12-9917 was used in Flow cytometry/Cell sorting to investigate the DC receptors involved in the uptake of P. gingivalis and the induced effector immune response.

Sun Nov 01 00:00:00 EDT 2009

Lipopolysaccharide induces IL-6 production in respiratory syncytial virus-infected airway epithelial cells through the toll-like receptor 4 signaling pathway.

Pediatric research

Xie XH,Law HK,Wang LJ,Li X,Yang XQ,Liu EM

12-9917 was used in Flow cytometry/Cell sorting to indicate that Toll-like receptor 4 plays a critical role in leading lipopolysaccharide mediated-IL-6 response in Respiratory syncytial virus infected-epithelial cells.

Sun Feb 01 00:00:00 EST 2009

Cigarette smoke regulates the expression of TLR4 and IL-8 production by human macrophages.

Journal of inflammation (London, England)

Sarir H,Mortaz E,Karimi K,Kraneveld AD,Rahman I,Caldenhoven E,Nijkamp FP,Folkerts G

12-9917 was used in Flow cytometry/Cell sorting to evaluate the effects of cigarette smoke medium on TLR4 expression and interleukin (IL)-8 production by human macrophages.

Fri May 01 00:00:00 EDT 2009

Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis.

Critical care (London, England)

Martins PS,Brunialti MK,Martos LS,Machado FR,Assunçao MS,Blecher S,Salomao R

12-9917 was used in Flow cytometry/Cell sorting to demonstrate that expression of surface receptors on neutrophils might be modulated across different severities of sepsis depending on the receptor.

Mon Jun 23 00:00:00 EDT 2008

The Journal of investigative dermatology

Mandron M,Ariès MF,Boralevi F,Martin H,Charveron M,Taieb A,Davrinche C

12-9917 was used in Flow cytometry/Cell sorting to assess monocytes sensitivity to SEB and LPS in a group of children and adult patients with dermatitis.

Tue Apr 01 00:00:00 EDT 2008

Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity.

Journal of leukocyte biology

Peiser M,Koeck J,Kirschning CJ,Wittig B,Wanner R

12-9917 was used in Flow cytometry/Cell sorting to analyse the Toll-like receptor (TLR) phenotype of epidermal Langerhans cells treated with various TLR agonists.

Thu May 01 00:00:00 EDT 2008

A phosphatidylserine species inhibits a range of TLR- but not IL-1beta-induced inflammatory responses by disruption of membrane microdomains.

Journal of immunology (Baltimore, Md. : 1950)

Parker LC,Prestwich EC,Ward JR,Smythe E,Berry A,Triantafilou M,Triantafilou K,Sabroe I

12-9917 was used in Flow cytometry/Cell sorting to investigate the ability of SAPS to inhibit the proinflammatory effects of TLR agonists in models of inflammation.

Wed Oct 15 00:00:00 EDT 2008

Pulmonary surfactant protein A regulates TLR expression and activity in human macrophages.

Journal of immunology (Baltimore, Md. : 1950)

Henning LN,Azad AK,Parsa KV,Crowther JE,Tridandapani S,Schlesinger LS

12-9917 was used in Flow Cytometry to identify a critical role for surfactant protein A in modulating the lung inflammatory response by regulating macrophage TLR activity.

Sun Jun 15 00:00:00 EDT 2008

Differential regulation of CCL-11/eotaxin-1 and CXCL-8/IL-8 by gram-positive and gram-negative bacteria in human airway smooth muscle cells.

Respiratory research

Issa R,Sorrentino R,Sukkar MB,Sriskandan S,Chung KF,Mitchell JA

12-9917 was used in Flow cytometry/Cell sorting to show that Gram-positive or Gram-negative bacteria cause human airway smooth muscle cells to release the neutrophil selective chemokine CXCL-8.

Tue Apr 01 00:00:00 EDT 2008

Selective impairment of Toll-like receptor 2-mediated proinflammatory cytokine production by monocytes from patients with atopic dermatitis.

The Journal of allergy and clinical immunology

Hasannejad H,Takahashi R,Kimishima M,Hayakawa K,Shiohara T

12-9917 was used in Flow cytometry/Cell sorting to demonstrate that monocytes from atopic dermatitis patients are defective in their capacity to produce proinflammatory cytokines on TLR2 stimuli.

Sun Jul 01 00:00:00 EDT 2007

Immaturity of infection control in preterm and term newborns is associated with impaired toll-like receptor signaling.

The Journal of infectious diseases

Sadeghi K,Berger A,Langgartner M,Prusa AR,Hayde M,Herkner K,Pollak A,Spittler A,Forster-Waldl E

12-9917 was used in Flow cytometry/Cell sorting to show that the reduced functional response to components of the bacterial cell wall might predispose premature newborns to bacterial infection.

Mon Jan 15 00:00:00 EST 2007

Changes in Toll-like receptor (TLR)-2 and TLR4 expression and function but not polymorphisms are associated with acute anterior uveitis.

Investigative ophthalmology & visual science

Chang JH,Hampartzoumian T,Everett B,Lloyd A,McCluskey PJ,Wakefield D

12-9917 was used in Flow cytometry/Cell sorting to suggest that microbial triggers and TLRs play a role in the pathogenesis of acute anterior uveitis.

Sun Apr 01 00:00:00 EDT 2007

Toll-like receptor 2, 3, and 4 expression and function in human airway smooth muscle.

The Journal of allergy and clinical immunology

Sukkar MB,Xie S,Khorasani NM,Kon OM,Stanbridge R,Issa R,Chung KF

12-9917 was used in Flow cytometry/Cell sorting to determine the effects of cytokines, TLR ligands, and corticosteroids on TLR expression in human airway smooth muscle cells.

Fri Sep 01 00:00:00 EDT 2006

Human heat shock protein 60 stimulates vascular smooth muscle cell proliferation through Toll-like receptors 2 and 4.

Microbes and infection

de Graaf R,Kloppenburg G,Kitslaar PJ,Bruggeman CA,Stassen F

Thu Jun 01 00:00:00 EDT 2006

Expression of Toll-like receptor 2 is up-regulated in monocytes from patients with chronic obstructive pulmonary disease.

Respiratory research

Pons J,Sauleda J,Regueiro V,Santos C,López M,Ferrer J,Agustí AG,Bengoechea JA

12-9917 was used in Flow cytometry/Cell sorting to reveal abnormalities in TLRs expression in chronic obstructive pulmonary disease patients and highlight its potential relationship with systemic inflammation.

Mon Apr 10 00:00:00 EDT 2006

Expansion of toll-like receptor 9-expressing B cells in active systemic lupus erythematosus: implications for the induction and maintenance of the autoimmune process.

Arthritis and rheumatism

Papadimitraki ED,Choulaki C,Koutala E,Bertsias G,Tsatsanis C,Gergianaki I,Raptopoulou A,Kritikos HD,Mamalaki C,Sidiropoulos P,Boumpas DT

12-9917 was used in Flow cytometry/Cell sorting to examine the expression and function of various TLRs in subpopulations of peripheral blood mononuclear cells of patients with systemic lupus erythematosus.

Wed Nov 01 00:00:00 EST 2006

Platelets express functional Toll-like receptor-4.

Blood

Andonegui G,Kerfoot SM,McNagny K,Ebbert KV,Patel KD,Kubes P

12-9917 was used in Flow cytometry/Cell sorting to study platelets expressing functional levels of TLR4, which contribute to thrombocytopenia through neutrophil-dependent pulmonary sequestration in response to LPS.

Sat Oct 01 00:00:00 EDT 2005

Neonatal immune responses to microbial stimuli: is there an influence of maternal allergy?

The Journal of allergy and clinical immunology

Amoudruz P,Holmlund U,Malmström V,Trollmo C,Bremme K,Scheynius A,Sverremark-Ekström E

12-9917 was used in Flow cytometry/Cell sorting to suggest that cord blood monocytes and cord blood mononuclear blood cell immune responses are influenced by maternal allergy.

Wed Jun 01 00:00:00 EDT 2005

Regulation of Toll-like receptor (TLR)2 and TLR4 on CD14dimCD16+ monocytes in response to sepsis-related antigens.

Clinical and experimental immunology

Skinner NA,MacIsaac CM,Hamilton JA,Visvanathan K

12-9917 was used in Flow cytometry/Cell sorting to hypothesize that following exposure to bacterial antigens, subsequent TNF-alpha drives a differentiation of monocytes into a CD14(dim)CD16(+) subpopulation.

Mon Aug 01 00:00:00 EDT 2005

Monocyte toll-like receptor 4 expression and LPS-induced cytokine production increase during gestational aging.

Pediatric research

Förster-Waldl E,Sadeghi K,Tamandl D,Gerhold B,Hallwirth U,Rohrmeister K,Hayde M,Prusa AR,Herkner K,Boltz-Nitulescu G,Pollak A,Spittler A

12-9917 was used in Flow cytometry/Cell sorting to conclude that, in very low birth weight infants, the minimized TLR4 expression contributes to their susceptibility to Gram-negative bacterial infections.

Fri Jul 01 00:00:00 EDT 2005

Peptidoglycan of Staphylococcus aureus upregulates monocyte expression of CD14, Toll-like receptor 2 (TLR2), and TLR4 in human blood: possible implications for priming of lipopolysaccharide signaling.

Infection and immunity

Hadley JS,Wang JE,Foster SJ,Thiemermann C,Hinds CJ

12-9917 was used in Flow cytometry/Cell sorting to show that TLR2 and TLR4 upregulation precedes the synergistic effects of lipopolysaccharide and peptidoglycan on cytokine release.

Tue Nov 01 00:00:00 EST 2005

Membrane-anchored CD14 is important for induction of interleukin-8 by lipopolysaccharide and peptidoglycan in uroepithelial cells.

Clinical and diagnostic laboratory immunology

Shimizu T,Yokota S,Takahashi S,Kunishima Y,Takeyama K,Masumori N,Takahashi A,Matsukawa M,Itoh N,Tsukamoto T,Fujii N

12-9917 was used in Flow cytometry/Cell sorting to identify factors promoting IL-8 induction in bladder epithelial cells in response to bacterial components.

Wed Sep 01 00:00:00 EDT 2004

Gamma interferon augments the intracellular pathway for lipopolysaccharide (LPS) recognition in human intestinal epithelial cells through coordinated up-regulation of LPS uptake and expression of the intracellular Toll-like receptor 4-MD-2 complex.

Infection and immunity

Suzuki M,Hisamatsu T,Podolsky DK

12-9917 was used in Flow cytometry/Cell sorting to demonstrate that commonly studied human intestinal epithelial cell lines can be classified into at least three different types.