Invitrogen

CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), Biotin, eBioscience™

CD152 (CTLA-4) Antibody in Flow Cytometry (Flow)

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CD152 (CTLA-4) Antibody (13-1522-82) in Flow

Frontiers in immunology 2023 - Rig-like helicase (RLH) activation augments co-stimulation and inhibits CTLA-4, B7-1 and B7-2. WT mice were used to evaluate the effects of Rig-like helicase activation with Poly (I:C) on co-stimulation and the CTLA-4 axis. (A) RT-PCR was used to quantitate the levels of mRNA encoding ICOS, ICOS L and CD28 in the lungs from WT mice that received B16 cells (B16+) or vehicle control (B16-) and were treated wi... View More

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产品信息

13-1522-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

UC10-4B9

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466619

产品详细信息

Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

Applications Reported: The UC10-4B9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The UC10-4B9 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。