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Invitrogen
14-3-3 beta Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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14-3-3 beta Antibody (PA1-4647) in ICC/IF
Immunofluorescence analysis of YWHAB was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with 14-3-3 beta Polyclonal Antibody (Product # PA1-4647) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius ove... View More
产品信息
PA1-4647
产品规格
种属反应
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to the C-terminal region of rat YWHAB conjugated to KLH
偶联物
形式
Liquid
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2218098
产品详细信息
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus and zebrafish based on 100% sequence homology.
This antibody is specific for the ~29 kDa 14-3-3 protein. Immunolabeling is blocked by preadsorption of the antibody with the protein used to generate the antibody.
Not recommended for use in paraffin sections.
靶标信息
14-3-3, a family of acidic and soluble proteins, highly conserved in amino acid sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms(beta, gamma, epsilon, eta, zeta, sigma and tau/theta) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various biological processes such as signal trans-duction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 14-3-3 protein beta-subtype; 14-3-3 protein beta/alpha; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; KCIP 1; KCIP-1; KCIP1; Prepronerve growth factor RNH-1; Protein kinase C inhibitor protein 1; RP1-148E22.1; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide
基因别名: Ywhab
UniProt ID: (Rat) P35213
Entrez Gene ID: (Rat) 56011
