Invitrogen

14-3-3 beta Monoclonal Antibody (05)

14-3-3 beta Antibody in Western Blot (WB)

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14-3-3 beta Antibody (MA5-28985) in WB

Western Blot using 14-3-3 beta Monoclonal Antibody (5) (Product # MA5-28985) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 32 kDa.

14-3-3 beta Antibody in Immunoprecipitation (IP)

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产品信息

MA5-28985

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1:1,000-1:2,000

免疫沉淀 (IP)

4-6 µL/mg of lysate

产品规格

种属反应

Cynomolgus monkey

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

抗原

Recombinant Cynomolgus 14-3-3 beta/YWHAB protein (Met2-Asn244)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2784925

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Cynomolgus 14-3-3 beta/YWHAB.

靶标信息

14-3-3, a family of acidic and soluble proteins, highly conserved in amino acid sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms(beta, gamma, epsilon, eta, zeta, sigma and tau/theta) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various biological processes such as signal trans-duction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.

仅用于科研。不用于诊断过程。未经明确授权不得转售。