Invitrogen

14-3-3 beta/zeta Recombinant Rabbit Monoclonal Antibody (7Z4S5)

14-3-3 beta/zeta Antibody in Western Blot (WB)

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14-3-3 beta/zeta Antibody (MA5-42912) in WB

Western blot analysis of 14-3-3 beta/zeta in extracts of various cell lines. Samples were incubated with 14-3-3 beta/zeta Monoclonal antibody (Product # MA5-42912) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

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产品信息

MA5-42912

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

7Z4S5

抗原

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 14-3-3 beta/zeta (P31946)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.28 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2912053

产品详细信息

Positive test controls include: HT-29, A-549, NIH/3T3, Jurkat, Mouse brain, Mouse kidney, Rat lung, Rat brain, Rat spleen.

Immunogen sequence: MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL

靶标信息

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 14-3-3 alpha; 14-3-3 delta; 14-3-3 protein beta; 14-3-3 protein beta-subtype; 14-3-3 protein beta/alpha; 14-3-3 protein zeta/delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; 14-3-3d; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; brain protein 14-3-3, beta isoform; epididymis luminal protein 4; epididymis secretory protein Li 1; epididymis secretory protein Li 3; epididymis secretory protein Li 93; KCIP-1; Mitochondrial import stimulation factor S1 subunit; phospholipase A2; Prepronerve growth factor RNH-1; Protein 1054; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; SEZ-2; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; YWHAE

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基因别名: 1110013I11Rik; 1300003C17Rik; 14-3-3-zeta; 14-3-3z; 14-3-3zeta; AI596267; AL022924; AU020854; GW128; HEL-S-1; HEL-S-3; HEL-S-93; HEL4; HS1; KCIP-1; Msfs1; YWHAA; YWHAB; YWHAD; YWHAZ