Invitrogen

14-3-3 gamma Monoclonal Antibody (KC21)

14-3-3 gamma Antibody in Western Blot (WB)

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14-3-3 gamma Antibody (MA1-16587) in WB

Western blot analysis of 14-3-3 gamma in (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates. Samples were incubated in 14-3-3 gamma monoclonal antibody (Product # MA1-16587) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody at a dilution of 1:10,000. The protein was detected at approximately 28 kDa after incubation with chemiluminescent substrate.

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产品信息

MA1-16587

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

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免疫细胞化学 (ICC/IF)

1:500

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

KC21

抗原

N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_568418

产品详细信息

Suggested positive control: antigen standard for YWHAG (transient overexpression lysate), HeLa whole cell extract.

靶标信息

14-3-3 gamma encodes a product that belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection.

Cells

Ercan H,Resch U,Hsu F,Mitulovic G,Bileck A,Gerner C,Yang JW,Geiger M,Miller I,Zellner M

Published figure using 14-3-3 gamma monoclonal antibody (Product # MA1-16587) in Western Blot

Sun Feb 26 00:00:00 EST 2023

Identification and validation of platelet low biological variation proteins, superior to GAPDH, actin and tubulin, as tools in clinical proteomics.

Journal of proteomics

Baumgartner R,Umlauf E,Veitinger M,Guterres S,Rappold E,Babeluk R,Mitulović G,Oehler R,Zellner M

MA1-16587 was used in western blot to study the platelet proteome to identify proteins with low variability suitable for use as quantitation controls in clinical proteomic investigations

Fri Dec 06 00:00:00 EST 2013

生物信息学

蛋白别名: 14-3-3 gamma; 14-3-3 protein gamma; KCIP-1; Protein kinase C inhibitor protein 1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide

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基因别名: 14-3-3GAMMA; PPP1R170; YWHAG

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UniProt ID: (Human) P61981

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Entrez Gene ID: (Human) 7532

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功能

protein kinase C binding insulin-like growth factor receptor binding protein binding protein kinase C inhibitor activity protein domain specific binding receptor tyrosine kinase binding poly(A) RNA binding scaffold/adaptor protein

参与通路

G2/M transition of mitotic cell cycle negative regulation of protein kinase activity protein targeting regulation of signal transduction cellular response to insulin stimulus regulation of neuron differentiation regulation of synaptic plasticity membrane organization negative regulation of protein serine/threonine kinase activity positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway