Invitrogen

NOTCH1 Monoclonal Antibody (mN1A), eBioscience™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3个已发表图片

10 篇参考文献

查看其他31个NOTCH1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 7

NOTCH1 Antibody (14-5785-81) in WB

Western blot was performed using Anti-NOTCH1 Monoclonal Antibody (mN1A), eBioscience™ (Product # 14-5785-81) and a 110 kDa band corresponding to NOTCH1 was observed across in MCF 10A and MOLT-4 in comparison to MDA-MB-231, MCF-7 and Jurkat which are reported to be negative . Whole cell extracts (30 µg lysate) of MCF 10A (Lane 1), MDA-MB-231 (Lane 2), MCF-7 (Lane 3), MOLT-4 (Lane 4) and Jurkat (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocel... View More

NOTCH1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

产品信息

14-5785-81

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

mN1A

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_467579

产品详细信息

Description: The Notch family of transmembrane receptors controls cell-fate decisions during the development of many organs in a wide variety of species. After binding its ligand, the Notch receptor is cleaved in its transmembrane domain, and the resulting intracellular domain dissociates from the membrane and translocates to the nucleus, where it is able to suppress the expression of lineage-specific genes by interacting with transcriptional repressors. The mN1A antibody reacts with the intracellular domain of mouse and human Notch1, but not with Notch2, 3, or 4. The mN1A antibody has a low affinity for the full-length (unprocessed or heterodimeric cell surface) forms of Notch1. In the mouse, Notch mRNA is expressed in mouse hematopoietic cells of the fetal liver and adult thymus and bone marrow. In the thymus, Notch1 protein is detected in CD4-CD8- (double-negative) and CD4-CD8+ (single-positive) thymocytes. Studies of Notch1-transgenic cells and Notch1-null mice indicate that the receptor is involved in the regulation of lymphopoiesis and myelopoiesis.

Applications Reported: This mN1A antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. (Fluorochrome conjugated mN1A is recommended for use in intracellular flow cytometry.).

Applications Tested: This mN1A antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The c-Myc Oncogene Maintains Corneal Epithelial Architecture at Homeostasis, Modulates p63 Expression, and Enhances Proliferation During Tissue Repair.

Investigative ophthalmology & visual science

Portal C,Wang Z,Scott DK,Wolosin JM,Iomini C

14-5785-81 was used in Western Blot to show that Myc regulates the balance among CE stratification, differentiation, and surface exfoliation and promotes the transition to the hyperproliferative state during wound healing.

Tue Feb 01 00:00:00 EST 2022

Genetic alterations of Keap1 confers chemotherapeutic resistance through functional activation of Nrf2 and Notch pathway in head and neck squamous cell carcinoma.

Cell death & disease

Islam SS,Qassem K,Islam S,Parag RR,Rahman MZ,Farhat WA,Yeger H,Aboussekhra A,Karakas B,Noman ASM

14-5785-81 was used in Western Blotting to suggest that Keap1 mutations define a distinct disease phenotype and the Keap1-Nrf2 pathway is one of the leading molecular mechanisms for clinical chemotherapeutic resistance.

Tue Aug 09 00:00:00 EDT 2022

Genetic alterations of Keap1 confers chemotherapeutic resistance through functional activation of Nrf2 and Notch pathway in head and neck squamous cell carcinoma.

Cell death & disease

Islam SS,Qassem K,Islam S,Parag RR,Rahman MZ,Farhat WA,Yeger H,Aboussekhra A,Karakas B,Noman ASM

Tue Aug 09 00:00:00 EDT 2022

Genetic alterations of Keap1 confers chemotherapeutic resistance through functional activation of Nrf2 and Notch pathway in head and neck squamous cell carcinoma.

Cell death & disease

Islam SS,Qassem K,Islam S,Parag RR,Rahman MZ,Farhat WA,Yeger H,Aboussekhra A,Karakas B,Noman ASM

Tue Aug 09 00:00:00 EDT 2022

Whole Exome Sequencing reveals NOTCH1 mutations in anaplastic large cell lymphoma and points to Notch both as a key pathway and a potential therapeutic target.

Haematologica

Larose H,Prokoph N,Matthews JD,Schlederer M,Högler S,Alsulami AF,Ducray SP,Nuglozeh E,Fazaludeen FMS,Elmouna A,Ceccon M,Mologni L,Gambacorti-Passerini C,Hoefler G,Lobello C,Pospisilova S,Janikova A,Woessmann W,Damm-Welk C,Zimmermann M,Federova A,Malone A,Smith O,Wasik M,Inghirami G,Lamant L,Blundell TL,Klapper W,Merkel O,Burke AGA,Mian S,Ashankyty I,Kenner L,Turner SD

14-5785-81 was used in Western Blot to identify a variant in the extracellular domain of NOTCH1 that provides a growth advantage to cells and confirm the suitability of the Notch pathway as a second-line druggable target in Anaplastic Lymphoma Kinase and Anaplastic Large Cell Lymphoma, using Whole-Exome Sequencing.

Tue Jun 01 00:00:00 EDT 2021

Whole Exome Sequencing reveals NOTCH1 mutations in anaplastic large cell lymphoma and points to Notch both as a key pathway and a potential therapeutic target.

Haematologica

Tue Jun 01 00:00:00 EDT 2021

FOXP3 expression is modulated by TGF‑β1/NOTCH1 pathway in human melanoma.

International journal of molecular medicine

Skarmoutsou E,Bevelacqua V,D' Amico F,Russo A,Spandidos DA,Scalisi A,Malaponte G,Guarneri C

14-5785 was used in Western Blotting to demonstrate that NOTCH1 is a central mediator of TGF-β-mediated FOXP3 expression and NOTCH1 inhibition reduces melanoma cell proliferation and viability.

Sun Jul 01 00:00:00 EDT 2018

FOXP3 expression is modulated by TGF‑β1/NOTCH1 pathway in human melanoma.

International journal of molecular medicine

Skarmoutsou E,Bevelacqua V,D' Amico F,Russo A,Spandidos DA,Scalisi A,Malaponte G,Guarneri C

14-5785 was used in Western Blotting to demonstrate that NOTCH1 is a central mediator of TGF-β-mediated FOXP3 expression and NOTCH1 inhibition reduces melanoma cell proliferation and viability.

Sun Jul 01 00:00:00 EDT 2018

Therapeutic targeting of NOTCH signaling ameliorates immune-mediated bone marrow failure of aplastic anemia.

The Journal of experimental medicine

Roderick JE,Gonzalez-Perez G,Kuksin CA,Dongre A,Roberts ER,Srinivasan J,Andrzejewski C,Fauq AH,Golde TE,Miele L,Minter LM

14-5785 was used in Flow cytometry/Cell sorting to identify NOTCH signaling as a primary driver of Th1-mediated pathogenesis in AA and may represent a novel target for therapeutic intervention.

Mon Jul 01 00:00:00 EDT 2013

Novel protein transduction domain mimics as nonviral delivery vectors for siRNA targeting NOTCH1 in primary human T cells.

Molecular therapy : the journal of the American Society of Gene Therapy

Tezgel AÖ,Gonzalez-Perez G,Telfer JC,Osborne BA,Minter LM,Tew GN

14-5785 was used in Western Blotting to develop a novel system for intracellular delivery of siRNA molecules.

Tue Jan 01 00:00:00 EST 2013

Dll4-Notch signaling in Flt3-independent dendritic cell development and autoimmunity in mice.

The Journal of experimental medicine

Billiard F,Lobry C,Darrasse-Jèze G,Waite J,Liu X,Mouquet H,DaNave A,Tait M,Idoyaga J,Leboeuf M,Kyratsous CA,Burton J,Kalter J,Klinakis A,Zhang W,Thurston G,Merad M,Steinman RM,Murphy AJ,Yancopoulos GD,Aifantis I,Skokos D

14-5785 was used in Flow cytometry/Cell sorting to investigate how Dll4-Notch signaling affects pro-T cell fate and thymic DC development, showing that it is a novel Flt3-independent pathway for DC development and autoimmunity.

Mon May 07 00:00:00 EDT 2012

Notch signaling regulates mouse and human Th17 differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Keerthivasan S,Suleiman R,Lawlor R,Roderick J,Bates T,Minter L,Anguita J,Juncadella I,Nickoloff BJ,Le Poole IC,Miele L,Osborne BA

14-5785 was used as a Control in experiments to highlight the importance of Notch signaling in Th17 differentiation and indicate that selective targeted therapy against Notch may be an important tool to treat autoimmune disorders, including multiple sclerosis.

Fri Jul 15 00:00:00 EDT 2011

Pathways contributing to development of spontaneous mammary tumors in BALB/c-Trp53+/- mice.

The American journal of pathology

Yan H,Blackburn AC,McLary SC,Tao L,Roberts AL,Xavier EA,Dickinson ES,Seo JH,Arenas RB,Otis CN,Cao QJ,Lawlor RG,Osborne BA,Kittrell FS,Medina D,Jerry DJ

14-5785 was used in Western Blotting to produce a panel of 28 transplantable tumors as resource for preclinical testing of therapeutic agents targeting cancer stem cells or oncogenic changes occurring during the progression from locally invasive to metastatic breast cancer.

Mon Mar 01 00:00:00 EST 2010

生物信息学

蛋白别名: major type A protein; Motch A; mT14; Neurogenic locus notch homolog protein 1; Notch 1; Notch gene homolog 1; Notch homolog 1, translocation-associated; p300; RP23-306D20.12; RP23-306D20.12-001; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1

查看更多收起

基因别名: 9930111A19Rik; AOS5; AOVD1; hN1; lin-12; Mis6; Motch; N1; NOTCH1; TAN1

查看更多收起

UniProt ID: (Human) P46531, (Mouse) Q01705

查看更多收起

Entrez Gene ID: (Human) 4851, (Mouse) 18128

查看更多收起

功能