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Invitrogen
Neural/Glial Antigen 2 (NG2) Monoclonal Antibody (9.2.27), eBioscience™
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Neural/Glial Antigen 2 (NG2) Antibody (14-6504-82) in ICC/IF
Immunofluorescence analysis of NG2 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Anti-NG2 Monoclonal Antibody (Product # 14-6504-80) at 10 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnig... View More
产品信息
14-6504-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Rat
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
9.2.27
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10870987
产品详细信息
Description: This 9.2.27 monoclonal antibody reacts with human neural/glial antigen 2, which is also known as melanoma chondroitin sulfate proteoglycan (MCSP). This antigen is composed of a 250 kDa N-linked glycoprotein and a >450 kDa proteoglycan. NG2 is present on the surface of >90% of malignant melanomas in addition to some non-melanomic tumors. Moreover, NG2 is expressed on glioma cells, as well as on developing and adult oligodendrocyte precursor cells. Studies have demonstrated the involvement of this protein in tumor cell proliferation, adhesion, migration, and invasion. NG2 also functions as a coreceptor for alpha4beta1 integrin. Finally, expression of NG2 can be used as a prognostic marker for disorders such as acral letiginous melanoma and infantile acute myeloid leukemia.
The 9.2.27 antibody has been reported to suppress melanoma tumor growth.
Applications Reported: This 9.2.27 has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin embedded tissue (IHC-P), and immunocytochemical staining of fixed cells (ICC).
Applications Tested: This 9.2.27 antibody has been tested by immunocytochemistry on methanol-fixed A375 cells at less than or equal to 10 µg/mL and by flow cytometric analysis on A375 cells at less than or equal to 0.125 µg/test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan NG2; HMW MAA; MEL CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan
基因别名: CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
UniProt ID: (Human) Q6UVK1
Entrez Gene ID: (Human) 1464
activation of MAPK activity
angiogenesis
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
glial cell migration
cell migration
glycosaminoglycan metabolic process
chondroitin sulfate biosynthetic process
chondroitin sulfate catabolic process
dermatan sulfate biosynthetic process
intracellular signal transduction
tissue remodeling
positive regulation of peptidyl-tyrosine phosphorylation
