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Invitrogen
Cutaneous Lymphocyte Antigen (CLA) Monoclonal Antibody (HECA-452), eBioscience™
Application
FIGURE: 1 / 1
Cutaneous Lymphocyte Antigen (CLA) Antibody (14-9857-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil, using 10 µg/mL Rat IgM Isotype Control (left) or 10 µg/mL Anti-Human/Mouse Cutaneous Lymphocyte Antigen (CLA) Purified (right) followed by biotinylated anti-rat IgG and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-9857-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgM, kappa
Class
Monoclonal
Type
Antibody
Clone
HECA-452
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573043
Product Specific Information
Description: This HECA-452 monoclonal antibody reacts with human Cutaneous Lymphocyte Antigen (CLA). This cell surface glycoprotein is most highly expressed on T cells in the skin. Nevertheless, CLA can also be detected on some peripheral T and NK cells, as well as monocytes, neutrophils and CD1a+ dendritic cells. Studies have determined that this antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA, which binds E-selectin and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1), is involved in T lymphocyte infiltration and adhesion at cutaneous sites of inflammation.
The HECA-452 antibody has been reported to inhibit sLex binding to E-selectin; however, this property is still under debate in the literature.
Applications Reported: This HECA-452 antibody has been reported for use in flow cytometric analysis, western blotting, functional assays, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This HECA-452 antibody has been tested by flow cytometric analysis on human PBMCs and by immunohistochemistry on FFPE human tissue. The HECA-452 antibody can be used at less than or equal to 0.125 µg/test for flow cytometry and at less than or equal to 10 µg/mL. The antibody performs optimally with low pH antigen retrieval but high pH antigen retrieval can also be used. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This cell surface glycoprotein is most highly expressed on T cells in the skin. Nevertheless, CLA can also be detected on some peripheral T and NK cells, as well as monocytes, neutrophils and CD1a+ dendritic cells. Studies have determined that this antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA, which binds E-selectin and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1), is involved in T lymphocyte infiltration and adhesion at cutaneous sites of inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CLA
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