Invitrogen

CD38 Monoclonal Antibody (HIT2), PE-Cyanine5, eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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14 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

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CD38 Antibody (15-0389-42) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC and CD38 PE-Cyanine5. As expected based on known relative expression patterns, CD38 clone HIT2 stains a subset of lymphocytes (pink), monocytes (orange) and granulocytes (blue).

Product Image Gallery

CD38 Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC and CD38 PE-Cyanine5. As expected based on known relative expression patterns, CD38 clone HIT2 stains a subset of lymphocytes (pink), monocytes (orange) and granulocytes (blue). {RE}

产品信息

15-0389-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

偶联物

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

激发/发射光谱

568/666 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1834381

产品详细信息

Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.

Applications Reported: The HIT2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Immunometabolic interference between cancer and COVID-19.

Frontiers in immunology

Consonni FM,Durante B,Manfredi M,Bleve A,Pandolfo C,Garlatti V,Vanella VV,Marengo E,Barberis E,Bottazzi B,Bombace S,My I,Condorelli G,Torri V,Sica A

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Tue Apr 18 00:00:00 EDT 2023

B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction.

Aging cell

Lee JL,Fra-Bido SC,Burton AR,Innocentin S,Hill DL,Linterman MA

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

Novel myeloma patient-derived xenograft models unveil the potency of anlotinib to overcome bortezomib resistance.

Frontiers in oncology

Yue Y,Cao Y,Mao X,Wang F,Fan P,Qian L,Guo S,Li F,Guo Y,Chen T,Lin Y,Dong W,Liu Y,Huang Y,Gu W

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Tue Aug 23 00:00:00 EDT 2022

Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen dysregulates expression of MCL-1 by targeting FBW7.

PLoS pathogens

Kim YJ,Kim Y,Kumar A,Kim CW,Toth Z,Cho NH,Lee HR

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Chronic myelomonocytic leukaemia stem cell transcriptomes anticipate disease morphology and outcome.

EBioMedicine

Wiseman DH,Baker SM,Dongre AV,Gurashi K,Storer JA,Somervaille TC,Batta K

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

IFNγ induces epigenetic programming of human T-bet^hi B cells and promotes TLR7/8 and IL-21 induced differentiation.

eLife

Zumaquero E,Stone SL,Scharer CD,Jenks SA,Nellore A,Mousseau B,Rosal-Vela A,Botta D,Bradley JE,Wojciechowski W,Ptacek T,Danila MI,Edberg JC,Bridges SL,Kimberly RP,Chatham WW,Schoeb TR,Rosenberg AF,Boss JM,Sanz I,Lund FE

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Wed May 15 00:00:00 EDT 2019

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Analysis of human B-cell responses following ChAd63-MVA MSP1 and AMA1 immunization and controlled malaria infection.

Immunology

Elias SC,Choudhary P,de Cassan SC,Biswas S,Collins KA,Halstead FD,Bliss CM,Ewer KJ,Hodgson SH,Duncan CJ,Hill AV,Draper SJ

15-0389 was used in Flow cytometry/Cell sorting to confirm that mBC and antibody responses can be induced and boosted by blood-stage parasite exposure, in support of epidemiological studies on low-level parasite exposure.

Tue Apr 01 00:00:00 EDT 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD38 monoclonal antibody (Product # 15-0389-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Suppression of homeobox transcription factor VentX promotes expansion of human hematopoietic stem/multipotent progenitor cells.

The Journal of biological chemistry

Gao H,Wu X,Sun Y,Zhou S,Silberstein LE,Zhu Z

15-0389 was used in Flow cytometry/Cell sorting to study the mechanisms that regulate the expansion of human hematopoietic stem/multipotent progenitor cells, showing that VentX regulates critical cell cycle regulators and Wnt downstream genes previously implicated in HSC/MPP proliferation and expansion.

Fri Aug 24 00:00:00 EDT 2012

生物信息学

蛋白别名: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; NAD(+) nucleosidase; NAD+ nucleosidase; T10

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基因别名: ADPRC 1; ADPRC1; CD38

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UniProt ID: (Human) P28907

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Entrez Gene ID: (Human) 952

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功能

NAD+ nucleosidase activity transferase activity phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

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