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Invitrogen
CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), Functional Grade, eBioscience™
产品信息
16-0499-81
产品规格
种属反应
Human
已发表种属
Rhesus monkey
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
9F10
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_468973
产品详细信息
Description: The 9F10 monoclonal antibody reacts with human CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin beta7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).
Applications Reported: The 9F10 antibody has been reported for use in flow cytometric analysis. 9F10 has also been reported in in vitro functional assays.
Applications Tested: The 9F10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; ITGA-IV; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha
基因别名: CD49D; IA4; ITGA4
UniProt ID: (Human) P13612
Entrez Gene ID: (Human) 3676
cell-matrix adhesion involved in ameboidal cell migration
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
B cell differentiation
extracellular matrix organization
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
endodermal cell differentiation
receptor clustering
regulation of immune response
leukocyte migration
leukocyte tethering or rolling
diapedesis
cellular response to cytokine stimulus
negative regulation of protein homodimerization activity
import into cell
positive regulation of leukocyte tethering or rolling
clathrin-mediated extracellular exosome endocytosis
positive regulation of T cell migration
