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Invitrogen
CD366 (TIM3) Monoclonal Antibody (F38-2E2), Functional Grade, eBioscience™
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CD366 (TIM3) Antibody (16-3109-85) in WB
The Biochemical journal 2021 -
Figure 1. Ectopic expression of TIM-3 enhances activation of Jurkat cells. ( A ) Flow cytometry analysis of human TIM-3 expression in parental (black) and TIM-3 lentivirus-transduced NF-kappaB GFP reporter Jurkat cells (red), detected with phycoerythrin-conjugated TIM-3 antibody. The inset shows Western blot analysis of TIM-3 expression in the same cells as marked -- with three biological repeats of each. (... View More
产品信息
16-3109-85
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
F38-2E2
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573083
产品详细信息
Description: This F38-2E2 monoclonal antibody reacts with human CD366, also known as T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) or HAVCR2. This cell surface receptor is expressed on activated CD4+ T cell subsets (e.g. Th1, Th17, and Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells. Due to alternative splicing, CD366 exists as membrane-bound and soluble forms. Galectin-9 has been identified as the ligand for CD366. In humans, this receptor negatively regulates CD4+ T cells, influencing the secretion of some Th1- and Th17-related cytokines. CD366 has also been implicated in tolerance, autoimmune disease (e.g. multiple sclerosis), and HIV infection.
Applications Tested: This F38-2E2 antibody has been tested by flow cytometric analysis of stimulated human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD366; FLJ14428; HAVcr-2; Hepatitis A virus cellular receptor 2; kidney injury molecule-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM-3; TIMD-3
基因别名: CD366; HAVcr-2; HAVCR2; KIM-3; Tim-3; TIM3; TIMD-3; TIMD3
UniProt ID: (Human) Q8TDQ0
Entrez Gene ID: (Human) 84868
adaptive immune response
macrophage activation involved in immune response
natural killer cell tolerance induction
regulation of tolerance induction dependent upon immune response
negative regulation of T-helper 1 type immune response
negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
inflammatory response
negative regulation of gene expression
negative regulation of myeloid dendritic cell activation
negative regulation of NF-kappaB transcription factor activity
negative regulation of interferon-alpha production
negative regulation of interferon-gamma production
negative regulation of interleukin-2 production
negative regulation of interleukin-3 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of chemokine production
positive regulation of interferon-gamma production
positive regulation of interleukin-1 production
positive regulation of interleukin-4 production
negative regulation of natural killer cell activation
toll-like receptor 3 signaling pathway
toll-like receptor 7 signaling pathway
toll-like receptor 9 signaling pathway
positive regulation of T cell proliferation
negative regulation of T cell proliferation
positive regulation of macrophage activation
innate immune response
defense response to Gram-positive bacterium
maternal process involved in female pregnancy
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
negative regulation of granulocyte colony-stimulating factor production
negative regulation of defense response to bacterium
positive regulation of defense response to bacterium
positive regulation of NIK/NF-kappaB signaling
positive regulation of tumor necrosis factor secretion
negative regulation of immunological synapse formation
negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
