Invitrogen

MICA/B Monoclonal Antibody (6D4), Functional Grade, eBioscience™

MICA/B Antibody in Immunohistochemistry (IHC)

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MICA/B Antibody (16-5788-85) in IHC

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society 2008 - Published figure using MICA/B monoclonal antibody (Product # 16-5788-85) in Immunohistochemistry

MICA/B Antibody in Flow Cytometry (Flow)

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产品信息

16-5788-85

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化 (IHC)

Assay-Dependent

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流式细胞分析 (Flow)

0.25 µg/test

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免疫沉淀 (IP)

Assay-Dependent

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

6D4

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_494087

产品详细信息

Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.

Applications Reported: This 6D4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested: This 6D4 antibody has been tested by flow cytometric analysis of HeLa cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Published figure using MICA/B monoclonal antibody (Product # 16-5788-85) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Role of NKG2D in cytokine-induced killer cells against lung cancer.

Oncology letters

Yin X,Lu X,Xiuwen Z,Min Z,Xiao R,Mao Z,Zhang Q

Published figure using MICA/B monoclonal antibody (Product # 16-5788-85) in Flow Cytometry

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Protective roles of epithelial cells in the survival of adult T-cell leukemia/lymphoma cells.

The American journal of pathology

Miyatake Y,Oliveira AL,Jarboui MA,Ota S,Tomaru U,Teshima T,Hall WW,Kasahara M

16-5788 was used in Flow cytometry/Cell sorting to investigate the possible factors behind the observed high resistance to antileukaemic therapies in patients with ATL.

Wed May 01 00:00:00 EDT 2013

Evidence for no relevance of anti-major histocompatibility complex class I-related chain a antibodies in liver transplantation.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

Uzunel M,Kasimu H,Joshi M,Ge X,Liu J,Xu B,Jaksch M,Jorns C,Nowak G,Sumitran-Holgersson S

Published figure using MICA/B monoclonal antibody (Product # 16-5788-85) in Western Blot

Mon Dec 01 00:00:00 EST 2008

Evidence for no relevance of anti-major histocompatibility complex class I-related chain a antibodies in liver transplantation.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

Uzunel M,Kasimu H,Joshi M,Ge X,Liu J,Xu B,Jaksch M,Jorns C,Nowak G,Sumitran-Holgersson S

Published figure using MICA/B monoclonal antibody (Product # 16-5788-85) in Western Blot

Mon Dec 01 00:00:00 EST 2008

生物信息学

蛋白别名: HLA class I antigen; MHC class I chain-related protein A; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence B; MHC class I related sequence A; MHC class I-like molecule PERB11.2-IMX; MIC-A; MIC-B; MICA; MICB; sMICA; sMICB; soluble MICA; soluble MICB; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A

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基因别名: MIC-A; MICA; MICB; PERB11.1; PERB11.2

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UniProt ID: (Human) Q9TQA4, (Human) Q29980

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Entrez Gene ID: (Human) 100507436, (Human) 4277

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功能

antigen binding protein binding beta-2-microglobulin binding natural killer cell lectin-like receptor binding major histocompatibility complex protein

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