Invitrogen

MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Functional Grade, eBioscience™

MHC Class I (H-2Kd) Antibody in Flow Cytometry (Flow)

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MHC Class I (H-2Kd) Antibody (16-5957-82) in Flow

Communications biology 2021 - Fig. 7 NME1 promotes lung metastasis from patient tumor cells. a Schematic of experimental workflow to investigate the effect of NME1 overexpression on spontaneous metastasis from cultured PDX tumor cells. b The box plot shows normalized gene expression of NME1 in metastatic (red, n = 435 cells) and primary tumor cells (blue, n = 684 cells). The lower and upper hinges correspond to the first and third quart... View More

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产品信息

16-5957-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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功能检测 (Functional)

Assay-Dependent

抑制试剂 (IA)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

SF1-1.1.1

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10670098

产品详细信息

Description: This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.

This monoclonal antibody has been reported to block CD8 involvement in TCR-ligand binding.

Applications Reported: This SF1-1.1.1 antibody has been reported for use in functional assays.

Applications Tested: This SF1-1.1.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis.

Communications biology

Ma D,Hernandez GA,Lefebvre AEYT,Alshetaiwi H,Blake K,Dave KR,Rauf M,Williams JW,Davis RT,Evans KT,Longworth A,Masoud MYG,Lee R,Edwards RA,Digman MA,Kessenbrock K,Lawson DA

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 16-5957-82) in Flow Cytometry

Fri Nov 05 00:00:00 EDT 2021

Multipotency of mouse trophoblast stem cells.

Stem cell research & therapy

Hou M,Han J,Li G,Kwon MY,Jiang J,Emani S,Taglauer ES,Park JA,Choi EB,Vodnala M,Fong YW,Emani SM,Rosas IO,Perrella MA,Liu X

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 16-5957-82) in Flow Cytometry

Thu Feb 13 00:00:00 EST 2020

Targeting photodynamic and photothermal therapy to the endoplasmic reticulum enhances immunogenic cancer cell death.

Nature communications

Li W,Yang J,Luo L,Jiang M,Qin B,Yin H,Zhu C,Yuan X,Zhang J,Luo Z,Du Y,Li Q,Lou Y,Qiu Y,You J

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 16-5957-82) in Flow Cytometry

Fri Jul 26 00:00:00 EDT 2019

Lin^-CCR2⁺ hematopoietic stem and progenitor cells overcome resistance to PD-1 blockade.

Nature communications

Flores CT,Wildes TJ,Drake JA,Moore GL,Dean BD,Abraham RS,Mitchell DA

16-5957-82 was used in Inhibition Assays to elucidate a novel role for CCR2+ haematopoietic stem cells in overcoming brain tumour resistance to PD-1 checkpoint blockade and adoptive cellular therapy in multiple invasive brain tumour models.

Wed Oct 17 00:00:00 EDT 2018

Membrane-organizing protein moesin controls Treg differentiation and antitumor immunity via TGF-β signaling.

The Journal of clinical investigation

Ansa-Addo EA,Zhang Y,Yang Y,Hussey GS,Howley BV,Salem M,Riesenberg B,Sun S,Rockey DC,Karvar S,Howe PH,Liu B,Li Z

16-5957 was used in Flow cytometry/Cell sorting to investigate whether moesin plays a role during the generation of TGF-β-induced Tregs, showing that it controls Treg differentiation and antitumor immunity.

Mon Apr 03 00:00:00 EDT 2017

Identification of Candidate Tolerogenic CD8(+) T Cell Epitopes for Therapy of Type 1 Diabetes in the NOD Mouse Model.

Journal of diabetes research

Yu C,Burns JC,Robinson WH,Utz PJ,Ho PP,Steinman L,Frey AB

16-5957 was used in Flow cytometry/Cell sorting to investigate two predicted tolerogenic CD8(+) T cell epitopes from the NOD murine model that are pathogenic in type 1 diabetes.

Tue Dec 13 00:00:00 EST 2016

A therapeutic cancer vaccine against GL261 murine glioma.

Journal of translational medicine

Kindy MS,Yu J,Zhu H,Smith MT,Gattoni-Celli S

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 16-5957-82) in Flow Cytometry

Tue Jan 05 00:00:00 EST 2016

Trogocytosis-associated cell to cell spread of intracellular bacterial pathogens.

eLife

Steele S,Radlinski L,Taft-Benz S,Brunton J,Kawula TH

16-5957 was used in Flow cytometry/Cell sorting to study trogocytosis-associated cell to cell spread of intracellular bacterial pathogens.

Sat Jan 23 00:00:00 EST 2016

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 16-5957-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

1,25-Dihydroxyvitamin D3 promotes tolerogenic dendritic cells with functional migratory properties in NOD mice.

Journal of immunology (Baltimore, Md. : 1950)

Ferreira GB,Gysemans CA,Demengeot J,da Cunha JP,Vanherwegen AS,Overbergh L,Van Belle TL,Pauwels F,Verstuyf A,Korf H,Mathieu C

16-5957 was used in Flow cytometry/Cell sorting to investigate the tolerogenic profile and T-cell inhibitory capacity of 1,25D3-mDCs derived from diabetes-prone NOD animals.

Thu May 01 00:00:00 EDT 2014

Compensatory mechanisms allow undersized anchor-deficient class I MHC ligands to mediate pathogenic autoreactive T cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Lamont D,Mukherjee G,Kumar PR,Samanta D,McPhee CG,Kay TW,Almo SC,DiLorenzo TP,Serreze DV

16-5957 was used in Flow cytometry/Cell sorting to discover undersized peptides as previously unrecognised targets of auto-reactive CD8 T cells.

Mon Sep 01 00:00:00 EDT 2014

Intraperitoneal administration of a tumor-associated antigen SART3, CD40L, and GM-CSF gene-loaded polyplex micelle elicits a vaccine effect in mouse tumor models.

PloS one

Furugaki K,Cui L,Kunisawa Y,Osada K,Shinkai K,Tanaka M,Kataoka K,Nakano K

16-5957 was used in Blocking experiments to investigate the potential of a non-viral polyplex micelle-based DNA vaccine in mouse tumour models with different MHC haplotypes.

Wed Mar 04 00:00:00 EST 2015

Separation of graft-versus-host disease from graft-versus-leukemia responses by targeting CC-chemokine receptor 7 on donor T cells.

Blood

Coghill JM,Carlson MJ,Panoskaltsis-Mortari A,West ML,Burgents JE,Blazar BR,Serody JS

16-5957 was used in Flow cytometry/Cell sorting to investigate the role of MHC disparity between donor and recipient, in the attenuated graft-versus-host disease responses of murine T cells lacking CCR7.

Thu Jun 10 00:00:00 EDT 2010

生物信息学

蛋白别名: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MGC184092; MGC7052; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

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基因别名: H-2K; H-2K(d); H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01901

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Entrez Gene ID: (Mouse) 14972

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功能

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

参与通路

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development