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Invitrogen
CD184 (CXCR4) Monoclonal Antibody (2B11), Functional Grade, eBioscience™
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CD184 (CXCR4) Antibody (16-9991-85) in ICC/IF
Nature communications 2018 -
Fig. 4 Cxcr4 marks SHF progenitors in mouse PSC-derived spheroids and in embryos. a RNA-seq analysis of differentially expressed surface receptors between Hcn4-GFP + and Tbx1-cre, RFP + CPCs in vitro. We identified 240 differentially expressed surface receptors of which the top 55 are shown here (all with Benjamini-Hochberg adjusted p -value < 0.05). b qPCR analysis of Cxcr4 expression in Hcn4-GFP + ... View More
产品信息
16-9991-85
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
2B11
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_842781
产品详细信息
Description: The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. In addition it has been shown to play a role in lymphocyte migration. The ligand for CXCR4 is SDF1. CXCR4 is required for maintenance of granulocyte progenitors in the bone marrow and essential for B cell development. Expression of CXCR4 is different between mice and human. In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predominant expression is found on B lymphocytes.
The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor.
Applications Reported: This 2B11 antibody has been reported for use in flow cytometric analysis and functional assays.
Applications Tested: This 2B11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; chemokine (C-X-C) receptor 4; chemokine receptor 4; CXC; CXC motif chemokine; CXC-R4; CXCR-4; Fusin; HUMSTR; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; PB-CKR; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; Stromal cell-derived factor 1 receptor
基因别名: b2b220Clo; CD184; Cmkar4; Cxcr4; LESTR; PB-CKR; PBSF/SDF-1; Sdf1r
UniProt ID: (Mouse) P70658
Entrez Gene ID: (Mouse) 12767
patterning of blood vessels
ameboidal-type cell migration
neuron migration
epithelial cell development
chemotaxis
signal transduction
G-protein coupled receptor signaling pathway
germ cell development
nervous system development
brain development
motor neuron axon guidance
germ cell migration
organ morphogenesis
cell migration
calcium-mediated signaling
telencephalon cell migration
regulation of cell migration
T cell proliferation
neutrophil activation
regulation of programmed cell death
myelin maintenance
positive regulation of oligodendrocyte differentiation
regulation of chemotaxis
regulation of calcium ion transport
cardiac muscle contraction
chemokine-mediated signaling pathway
cellular response to cytokine stimulus
