Invitrogen

CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), APC, eBioscience™

64个已发表图片
75 篇参考文献
查看其他88个Integrin beta 1 (CD29)抗体
说明书
实验方案
技术支持
说明书
实验方案
技术支持

产品信息

17-0291-82

应用
建议稀释比
已发表文章

免疫细胞化学 (ICC/IF)

-
查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.25 µg/test
查看74篇已发表文章 74篇已发表文章
产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Armenian hamster / IgG

推荐的同型对照

Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

eBioHMb1-1 (HMb1-1)

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱 spectra

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1210793

产品详细信息

Description: The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1.

Applications Reported: This eBioHMb1-1 (HMb1-1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytonetric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: Beta OL; Beta oligodendroglia; CD29; Fibronectin receptor subunit beta; integrin beta 1 (fibronectin receptor beta); Integrin beta-1; Integrin beta1; integrin VLA-4 subunit beta; ITBG1D; VLA-4 subunit beta

查看更多 收起

基因别名: 4633401G24Rik; AA409975; AA960159; CD29; ENSMUSG00000051907; Fnrb; Gm9863; gpIIa; Itgb1

查看更多 收起

UniProt ID: (Mouse) Q8BVU1, (Rat) P49134

查看更多 收起

Entrez Gene ID: (Mouse) 16412, (Rat) 24511

查看更多 收起

功能
glycoprotein binding fibronectin binding protease binding actin binding receptor activity receptor binding integrin binding protein binding collagen binding kinase binding protein kinase binding protein domain specific binding protein complex binding peptide binding laminin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion integrin
参与通路
G1/S transition of mitotic cell cycle in utero embryonic development cell fate specification tissue homeostasis cell migration involved in sprouting angiogenesis cellular calcium ion homeostasis cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration visual learning regulation of collagen catabolic process positive regulation of cell-substrate adhesion positive regulation of neuron projection development cell migration formation of radial glial scaffolds positive regulation of cell migration neuron projection development negative regulation of cell projection organization cell-substrate adhesion protein transport within lipid bilayer heterotypic cell-cell adhesion negative regulation of Rho protein signal transduction positive regulation of apoptotic process stress fiber assembly positive regulation of MAPK cascade sarcomere organization negative regulation of cell differentiation negative regulation of neuron differentiation positive regulation of neuron differentiation positive regulation of endocytosis axon extension cardiac muscle tissue development dendrite morphogenesis positive regulation of peptidyl-tyrosine phosphorylation leukocyte tethering or rolling regulation of cell cycle cardiac muscle cell differentiation bicellular tight junction assembly positive regulation of establishment of protein localization to plasma membrane negative regulation of anoikis response to activity response to nutrient levels cell-cell adhesion mediated by integrin response to gonadotropin response to drug mesodermal cell differentiation maternal process involved in female pregnancy cellular response to mechanical stimulus cellular response to vitamin D cellular response to ionizing radiation response to transforming growth factor beta
guarantee_icon

性能保障

只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符，您即可享受免费退换货服务。*

了解更多
help_icon

随时为您服务

获取常见问题的专家解答，或者直接联系我们的技术专家，咨询应用、设备、常规产品使用问题。

联系技术支持