Invitrogen

CD63 Monoclonal Antibody (NVG-2), APC, eBioscience™

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CD63 Antibody (17-0631-82) in Flow

Intracellular staining of mouse resident peritoneal exudate cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse CD63 APC (right) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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产品信息

17-0631-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

NVG-2

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573150

产品详细信息

Description: The monoclonal antibody NVG-2 reacts with mouse CD63, also known as Lysosomal-Associated Membrane Protein 3 (LAMP-3) or tetraspanin 30 (TSPN30), a member of tetraspanin family of proteins characterized by four transmembrane domains. CD63 is expressed on a variety of cell types of hematopoietic lineage, e.g., granulocytes, B lymphocytes, platelets, as well as cells of non-hematopoietic origin. It can be found on the cell membrane, late endocytic vesicles, lysosomes, exosomes, and other specialized granules. On the cell surface, CD63 has been shown to interact with various proteins forming tetraspanin-enriched microdomains (TEM). Its high expression on the cell membrane may be indicative of cell activation, hence, CD63 is often used as an activation marker for basophils, platelets and other cells.

Applications Reported: This NVG-2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This NVG-2 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse resident peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Phosphatase Shp2 regulates biogenesis of small extracellular vesicles by dephosphorylating Syntenin.

Journal of extracellular vesicles

Zhang Y,Li Y,Liu P,Gong D,Zhou H,Li W,Zhang H,Zheng W,Xu J,Cheng H,Zhang X,Ke Y

17-0631 was used in Flow cytometry/Cell sorting to provide a basis for understanding the mechanism of sEV formation and relevant function in epithelial-macrophage crosstalk.

Mon Mar 01 00:00:00 EST 2021

Type I interferon limits mast cell-mediated anaphylaxis by controlling secretory granule homeostasis.

PLoS biology

Kobayashi T,Shimabukuro-Demoto S,Tsutsui H,Toyama-Sorimachi N

Published figure using CD63 monoclonal antibody (Product # 17-0631-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Identification of diverse astrocyte populations and their malignant analogs.

Nature neuroscience

John Lin CC,Yu K,Hatcher A,Huang TW,Lee HK,Carlson J,Weston MC,Chen F,Zhang Y,Zhu W,Mohila CA,Ahmed N,Patel AJ,Arenkiel BR,Noebels JL,Creighton CJ,Deneen B

17-0631 was used in Flow cytometry/Cell sorting to study the cellular heterogeneity of astrocytes.

Wed Mar 01 00:00:00 EST 2017

Type I IFN and not TNF, is Essential for Cyclic Di-nucleotide-elicited CTL by a Cytosolic Cross-presentation Pathway.

EBioMedicine

Lirussi D,Ebensen T,Schulze K,Trittel S,Duran V,Liebich I,Kalinke U,Guzmán CA

17-0631 was used in Immunocytochemistry-immunoflourescence to demonstrate that stimulation of interferon-γ-secreting CD8+ cytotoxic T lymphocytes is significantly decreased after vaccination in the absence of type I interferon signalling.