Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), APC, eBioscience™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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30 篇参考文献

查看其他109个Transferrin Receptor (CD71)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

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CD71 (Transferrin Receptor) Antibody (17-0719-42) in Flow

Staining of 3-day anti-CD3/CD28-stimulated normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD71 (Transferrin Receptor) APC (purple histogram). Total viable cells were used for analysis.

Product Image Gallery

产品信息

17-0719-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

查看30篇已发表文章 30篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

OKT9 (OKT-9)

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10671393

产品详细信息

Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Applications Reported: This OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT9 (OKT-9) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

BMC biology

Rossatti P,Redpath GMI,Ziegler L,Samson GPB,Clamagirand CD,Legler DF,Rossy J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Wed Aug 24 00:00:00 EDT 2022

A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment.

NPJ precision oncology

Cornelison RC,Yuan JX,Tate KM,Petrosky A,Beeghly GF,Bloomfield M,Schwager SC,Berr AL,Stine CA,Cimini D,Bafakih FF,Mandell JW,Purow BW,Horton BJ,Munson JM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Fri Jul 29 00:00:00 EDT 2022

The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.

Development (Cambridge, England)

Creed TM,Baldeosingh R,Eberly CL,Schlee CS,Kim M,Cutler JA,Pandey A,Civin CI,Fossett NG,Kingsbury TJ

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Fri Jan 03 00:00:00 EST 2020

Phenotype changes of oral epithelial stem cells after in vitro culture.

Brazilian oral research

Daltoe FP,Oliveira NAJ,Peron CN,Sharpe PT,Mantesso A

17-0719 was used in Flow cytometry/Cell sorting to suggest that phenotype changes may be part of an intrinsic cellular mechanism to conserve levels of protein expression as they may found in the human body.

Thu Apr 23 00:00:00 EDT 2020

DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer.

JCI insight

de Cubas AA,Dunker W,Zaninovich A,Hongo RA,Bhatia A,Panda A,Beckermann KE,Bhanot G,Ganesan S,Karijolich J,Rathmell WK

17-0719 was used in Flow cytometry/Cell sorting to identify a potential strategy to induce TE expression through inhibition of DNA methylation in modulating T cell action via regulation of the innate antiviral pathway.

Thu Jun 04 00:00:00 EDT 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Rats provide a superior model of human stress erythropoiesis.

Experimental hematology

Zhang J,Liu Y,Han X,Mei Y,Yang J,Zhang ZJ,Lu X,Ji P

17-0719 was used in Flow cytometry/Cell sorting to establish rat in vitro and in vivo erythropoiesis models that are more appropriate and superior for the study of human stress erythropoiesis than mouse models.

Tue Oct 01 00:00:00 EDT 2019

Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

Cell reports

Ludwig LS,Lareau CA,Bao EL,Nandakumar SK,Muus C,Ulirsch JC,Chowdhary K,Buenrostro JD,Mohandas N,An X,Aryee MJ,Regev A,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.

Cell reports

Zaborowski MP,Lee K,Na YJ,Sammarco A,Zhang X,Iwanicki M,Cheah PS,Lin HY,Zinter M,Chou CY,Fulci G,Tannous BA,Lai CP,Birrer MJ,Weissleder R,Lee H,Breakefield XO

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism.

Developmental cell

Tanimura N,Liao R,Wilson GM,Dent MR,Cao M,Burstyn JN,Hematti P,Liu X,Zhang Y,Zheng Y,Keles S,Xu J,Coon JJ,Bresnick EH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Mon Sep 10 00:00:00 EDT 2018

EDAG promotes the expansion and survival of human CD34+ cells.

PloS one

Zhao K,Zheng WW,Dong XM,Yin RH,Gao R,Li X,Liu JF,Zhan YQ,Yu M,Chen H,Ge CH,Ning HM,Yang XM,Li CY

17-0719 was used in Flow cytometry/Cell sorting to provide novel insight into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells.

Tue Feb 06 00:00:00 EST 2018

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.

Cell

Kim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Dimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease.

JCI insight

Krishnamoorthy S,Pace B,Gupta D,Sturtevant S,Li B,Makala L,Brittain J,Moore N,Vieira BF,Thullen T,Stone I,Li H,Hobbs WE,Light DR

17-0719 was used in Flow cytometry/Cell sorting to investigate the ability of dimethyl fumarate to activate γ-globin transcription, and enhance foetal haemoglobin.

Thu Oct 19 00:00:00 EDT 2017

Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells.

Cell stem cell

Giani FC,Fiorini C,Wakabayashi A,Ludwig LS,Salem RM,Jobaliya CD,Regan SN,Ulirsch JC,Liang G,Steinberg-Shemer O,Guo MH,Esko T,Tong W,Brugnara C,Hirschhorn JN,Weiss MJ,Zon LI,Chou ST,French DL,Musunuru K,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Comparing the impact of an acute exercise bout on plasma amino acid composition, intraerythrocytic Ca(2+) handling, and red cell function in athletes and untrained subjects.

Cell calcium

Makhro A,Haider T,Wang J,Bogdanov N,Steffen P,Wagner C,Meyer T,Gassmann M,Hecksteden A,Kaestner L,Bogdanova A

17-0719-42 was used in Flow cytometry/Cell sorting to assess the potential physiological relevance of receptors with agonists triggered transient Ca(2+)-dependent decrease in hemoglobin oxygen affinity in RBC suspension.

Sat Oct 01 00:00:00 EDT 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

American journal of physiology. Cell physiology

Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A

17071942 was used in flow cytometry to investigate the impact of N-methyl-d-aspartate receptor on erythropoiesis

Mon Jun 15 00:00:00 EDT 2015

DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs.

BMC genomics

Aparicio-Prat E,Arnan C,Sala I,Bosch N,Guigó R,Johnson R

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Fri Oct 23 00:00:00 EDT 2015

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Oncogene

Zhang X,Cruz FD,Terry M,Remotti F,Matushansky I

17-0719 was used in Flow cytometry/Cell sorting to investigate the potential of nuclear reprogramming as a broadly applicable therapeutic strategy for the treatment of cancer.

Thu May 02 00:00:00 EDT 2013

UNC93B1 mediates differential trafficking of endosomal TLRs.

eLife

Lee BL,Moon JE,Shu JH,Yuan L,Newman ZR,Schekman R,Barton GM

17-0719 was used in Flow cytometry/Cell sorting to describe the mechanisms by which UNC93B1 regulates multiple aspects of Toll-like receptor trafficking, showing that post-Golgi trafficking of TLR7 and TLR9 are distinct.

Tue Feb 19 00:00:00 EST 2013

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Molecular and cellular biology

Ma Y,Wang B,Jiang F,Wang D,Liu H,Yan Y,Dong H,Wang F,Gong B,Zhu Y,Dong L,Yin H,Zhang Z,Zhao H,Wu Z,Zhang J,Zhou J,Yu J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

The Journal of biological chemistry

Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0719-42) in Flow Cytometry

Fri Jun 07 00:00:00 EDT 2013

Combined flow cytometric analysis of surface and intracellular antigens reveals surface molecule markers of human neuropoiesis.

PloS one

Turaç G,Hindley CJ,Thomas R,Davis JA,Deleidi M,Gasser T,Karaöz E,Pruszak J

17-0719 was used in Flow cytometry/Cell sorting to develop a validated protocol enabling the co-detection and co-expression of surface molecules on neural cell populations.