Invitrogen

IgD Monoclonal Antibody (11-26c (11-26)), APC, eBioscience™

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IgD Antibody (17-5993-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.06 µg of Anti-Mouse IgD APC (right). Total viable cells were used for analysis.

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产品信息

17-5993-82

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看13篇已发表文章 13篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Fish, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

11-26c (11-26)

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10598660

产品详细信息

Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.

Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

Published figure using IgD monoclonal antibody (Product # 17-5993-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Plasma Cell Fate Is Orchestrated by Elaborate Changes in Genome Compartmentalization and Inter-chromosomal Hubs.

Cell reports

Bortnick A,He Z,Aubrey M,Chandra V,Denholtz M,Chen K,Lin YC,Murre C

17-5993 was used in Flow cytometry/Cell sorting to examine whether plasma cell development is also associated with changes in nuclear architecture.

Tue Apr 07 00:00:00 EDT 2020

A Thpok-Directed Transcriptional Circuitry Promotes Bcl6 and Maf Expression to Orchestrate T Follicular Helper Differentiation.

Immunity

Vacchio MS,Ciucci T,Gao Y,Watanabe M,Balmaceno-Criss M,McGinty MT,Huang A,Xiao Q,McConkey C,Zhao Y,Shetty J,Tran B,Pepper M,Vahedi G,Jenkins MK,McGavern DB,Bosselut R

17-5993-82 was used in Flow Cytometry to identify a transcriptional program that links the CD4+ lineage with Tfh differentiation, a limiting factor for efficient B cell responses.

Tue Sep 17 00:00:00 EDT 2019

Cyclooxygenase-1 Regulates the Development of Follicular Th Cells via Prostaglandin E₂.

Journal of immunology (Baltimore, Md. : 1950)

Liu T,Yang Q,Cao YJ,Yuan WM,Lei AH,Zhou P,Zhou W,Liu YD,Shi MH,Yang Q,Tang JY,Wang HK,Zhang H,Yu Y,Zhou J

17-5993-82 was used in Flow Cytometry to indicate that COX-1 plays an important role in the development of TFH cells.

Thu Aug 15 00:00:00 EDT 2019

Mutations in topoisomerase IIβ result in a B cell immunodeficiency.

Nature communications

Broderick L,Yost S,Li D,McGeough MD,Booshehri LM,Guaderrama M,Brydges SD,Kucharova K,Patel NC,Harr M,Hakonarson H,Zackai E,Cowell IG,Austin CA,Hügle B,Gebauer C,Zhang J,Xu X,Wang J,Croker BA,Frazer KA,Putnam CD,Hoffman HM

17-5993-82 was used in Flow Cytometry to identify mutations in type II topoisomerase as a driver of defective proliferation and survival of B2 cells, leading to a B cell immunodeficiency.

Tue Aug 13 00:00:00 EDT 2019

Mast cell depletion in the preclinical phase of collagen-induced arthritis reduces clinical outcome by lowering the inflammatory cytokine profile.

Arthritis research & therapy

van der Velden D,Lagraauw HM,Wezel A,Launay P,Kuiper J,Huizinga TW,Toes RE,Bot I,Stoop JN

17-5993 was used in Flow cytometry/Cell sorting to suggest that mast cells may play a role in the regulation of the adaptive immune response during the development of arthritis.

Mon Jun 13 00:00:00 EDT 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using IgD monoclonal antibody (Product # 17-5993-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Meningeal Infiltration of the Spinal Cord by Non-Classically Activated B Cells is Associated with Chronic Disease Course in a Spontaneous B Cell-Dependent Model of CNS Autoimmune Disease.

Frontiers in immunology

Dang AK,Tesfagiorgis Y,Jain RW,Craig HC,Kerfoot SM

17-5993 was used in Immunohistochemistry on frozen tissues to characterise B cell infiltration of the spinal cord in a B cell-dependent spontaneous model of CNS autoimmunity, showing meningeal infiltration of the spinal cord by non-classically activated B cells.

Wed Oct 07 00:00:00 EDT 2015

BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.

PloS one

Funakoshi S,Shimizu T,Numata O,Ato M,Melchers F,Ohnishi K

Published figure using IgD monoclonal antibody (Product # 17-5993-82) in Flow Cytometry

Wed Sep 16 00:00:00 EDT 2015

CD40 ligand preferentially modulates immune response and enhances protection against influenza virus.

Journal of immunology (Baltimore, Md. : 1950)

Hashem AM,Gravel C,Chen Z,Yi Y,Tocchi M,Jaentschke B,Fan X,Li C,Rosu-Myles M,Pereboev A,He R,Wang J,Li X

17-5993 was used in Flow cytometry/Cell sorting to characterise an increased in vivo load of CD40-targeted Ag upon the secretion of the NP-CD40L fusion protein from adenovirus-infected cells.

Tue Jul 15 00:00:00 EDT 2014

Oral combined therapy with probiotics and alloantigen induces B cell-dependent long-lasting specific tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Mercadante AC,Perobelli SM,Alves AP,Gonçalves-Silva T,Mello W,Gomes-Santos AC,Miyoshi A,Azevedo V,Faria AM,Bonomo A

17-5993 was used in Flow cytometry/Cell sorting to suggest that combined therapy is a promising strategy for prevention of GVHD with preservation of graft-versus-leukemia in allogenic HSCT.

Sat Feb 15 00:00:00 EST 2014

Loss of an Igκ gene enhancer in mature B cells results in rapid gene silencing and partial reversible dedifferentiation.

Molecular and cellular biology

Zhou X,Xiang Y,Ding X,Garrard WT

Published figure using IgD monoclonal antibody (Product # 17-5993-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

The kinase TBK1 controls IgA class switching by negatively regulating noncanonical NF-κB signaling.

Nature immunology

Jin J,Xiao Y,Chang JH,Yu J,Hu H,Starr R,Brittain GC,Chang M,Cheng X,Sun SC

17-5993 was used in Flow cytometry/Cell sorting to study how immunoglobulin isotype switching is controlled, showing that kinase TBK1 is a pivotal negative regulator of class switching to the immunoglobulin A (IgA) isotype, by attenuating noncanonical signaling via the transcription factor NF-κB.

Thu Nov 01 00:00:00 EDT 2012

IKKα-mediated signaling circuitry regulates early B lymphopoiesis during hematopoiesis.

Blood

Balkhi MY,Willette-Brown J,Zhu F,Chen Z,Liu S,Guttridge DC,Karin M,Hu Y

17-5993-82 was used in Flow Cytometry to demonstrate that IKKα regulates B-lineage commitment via combined canonical and noncanonical NF-κB transcriptional activities to target Pax5 expression during hematopoiesis.

Thu Jun 07 00:00:00 EDT 2012

生物信息学

基因别名: IgD; Igh-5

查看更多收起

Entrez Gene ID: (Mouse) 380797

查看更多收起

IgD Monoclonal Antibody (11-26c (11-26)), APC, eBioscience™

IgD Antibody (17-5993-82) in Flow

产品信息

17-5993-82

免疫组化（冰冻） (IHC (F))

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

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