Invitrogen

CD217 (IL-17Ra) Monoclonal Antibody (PAJ-17R), APC, eBioscience™

CD217 (IL-17Ra) Antibody in Flow Cytometry (Flow)

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CD217 (IL-17Ra) Antibody (17-7182-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Mouse Ly-6G (Gr-1) PE (Product # 12-5931-82) and 0.125 µg of Rat IgG2a kappa Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse IL-17 Receptor APC (right). Total viable cells were used for analysis.

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产品信息

17-7182-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

PAJ-17R

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10597893

产品详细信息

Description: The monoclonal antibody PAJ-17R recognizes mouse IL-17 receptor A (IL-17RA) also known as CD217. This single transmembrane domain-containing receptor can homo- or heterodimerize with IL-17RC or IL-17RB. IL-17RA binds to IL-17A, IL-17F(cytokines of the Th 17 lineage), or IL-17E (IL-25). Ligand binding to the single subunit of IL-17RA has been shown to induce homo- or heterodimerization of the receptor complex that in turn recruits Act-1 via the SEFIR domains leading to recruitment of TRAF6 and signaling through the NFkappaB and MAPK pathways.

Initial reports suggested ubiquitous expression of IL-17RA based on binding studies with IL-17-Fc and expression of mRNA. Interestingly, despite this, not all cells expressing the receptor respond to IL-17A. In particular, resting T cells do not respond. It is not known if the expression level of IL-17RA protein varies or if some cells express the receptor but are not able to activate downstream signaling events. Recent evidence suggests a more limited expression profile based on IL-17RA knock-out mice which display decreased numbers of neutrophils with impaired function in response to challenge with K. pneumonia or Candida albicans. This suggests IL-17RA plays a more dominant and critical role in neutrophil development and function. Our studies suggest that expression can be found on abundantly on Gr-1^dim population and many of the Gr-1^bright bone marrow cells, as well as some F4/80 positive cells. This staining with PAJ-17R is not detected in IL-17RA knock-out mice.

Applications Reported: This PAJ-17R antibody has been reported for use in flow cytometric analysis.

Applications Tested: This PAJ-17R antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin 17A (IL-17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17RA is a ubiquitous type I membrane glycoprotein that binds with low affinity to IL-17A and IL-17F. IL-17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Defects in IL-17RA are the cause of familial candidiasis type 5 (CANDF5) which is a rare disorder with altered immune responses and impaired clearance of fungal infections.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Microbiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression.

Nature communications

Calcinotto A,Brevi A,Chesi M,Ferrarese R,Garcia Perez L,Grioni M,Kumar S,Garbitt VM,Sharik ME,Henderson KJ,Tonon G,Tomura M,Miwa Y,Esplugues E,Flavell RA,Huber S,Canducci F,Rajkumar VS,Bergsagel PL,Bellone M

17-7182 was used in Flow cytometry/Cell sorting to study how intestinal microbes influence progression of extramucosal tumours.

Mon Dec 03 00:00:00 EST 2018

Regulation of Inflammation by IL-17A and IL-17F Modulates Non-Alcoholic Fatty Liver Disease Pathogenesis.

PloS one

Giles DA,Moreno-Fernandez ME,Stankiewicz TE,Cappelletti M,Huppert SS,Iwakura Y,Dong C,Shanmukhappa SK,Divanovic S

17-7182 was used in Flow cytometry/Cell sorting to indicate that the IL-17 axis also plays a role in a MCDD-induced model of NAFLD pathogenesis.

Tue Jul 19 00:00:00 EDT 2016

Chronic oral infection with major periodontal bacteria Tannerella forsythia modulates systemic atherosclerosis risk factors and inflammatory markers.

Pathogens and disease

Chukkapalli SS,Rivera-Kweh MF,Velsko IM,Chen H,Zheng D,Bhattacharyya I,Gangula PR,Lucas AR,Kesavalu L

17-7182 was used in Flow cytometry/Cell sorting to investigate the potential of a known bacterial pathogen found in atherosclerotic plaque in humans to accelerate atherosclerosis in hyperlipdemic mice.

Wed Apr 01 00:00:00 EDT 2015

Local IL-17 Production Exerts a Protective Role in Murine Experimental Glomerulonephritis.

PloS one

Hamour S,Gan PY,Pepper R,Florez Barros F,Wang HH,O'Sullivan K,Iwakura Y,Cook T,Pusey C,Holdsworth S,Salama A

Published figure using CD217 (IL-17Ra) monoclonal antibody (Product # 17-7182-82) in Flow Cytometry

Wed May 11 00:00:00 EDT 2016

Polymicrobial Oral Infection with Four Periodontal Bacteria Orchestrates a Distinct Inflammatory Response and Atherosclerosis in ApoE null Mice.

PloS one

Chukkapalli SS,Velsko IM,Rivera-Kweh MF,Zheng D,Lucas AR,Kesavalu L

17-7182 was used in Flow cytometry/Cell sorting to study the levels of inflammatory molecules and cells types involved in mounting an immune response to peridontal infection over time.