Invitrogen

Arginase 1 Monoclonal Antibody (A1exF5), PE-Cyanine7, eBioscience™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

14个已发表图片

21 篇参考文献

查看其他62个Arginase 1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Arginase 1 Antibody in Flow Cytometry (Flow)

图: 1 / 16

Arginase 1 Antibody (25-3697-82) in Flow

Normal human lysed whole blood cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.25 µg of Arginase 1 Monoclonal Antibody, PE-Cyanine 7. Cells were co-stained with CD16 Monoclonal Antibody, FITC (Product # 11-0168-42) and CD193 Monoclonal Antibody, APC (Product # 17-1939-42). Cells in the granulocyte gate (left panel) were used for analysis and were gated on either the CD193+ population (blue histogram) or the CD16+ population (purple histogram).

Arginase 1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

25-3697-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看20篇已发表文章 20篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Fruit fly, Mouse, Rat

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

A1exF5

抗原

E.coli-derived recombinant mouse Arginase 1

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2734841

产品详细信息

Description: The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1). This A1exF5 clone is compatible with both, the standard intracellular protocols, and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: This A1exF5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A1exF5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-34) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

靶标信息

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Advanced phosphocreatine-grafted chitosan hydrogel promote wound healing by macrophage modulation.

Frontiers in bioengineering and biotechnology

Sheng W,Qin H,Wang T,Zhao J,Fang C,Zhang P,Liu P,Udduttula A,Zeng H,Chen Y

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Wed May 31 00:00:00 EDT 2023

Advanced phosphocreatine-grafted chitosan hydrogel promote wound healing by macrophage modulation.

Frontiers in bioengineering and biotechnology

Sheng W,Qin H,Wang T,Zhao J,Fang C,Zhang P,Liu P,Udduttula A,Zeng H,Chen Y

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Wed May 31 00:00:00 EDT 2023

Timing of Interleukin-4 Stimulation of Macrophages Determines Their Anti-Microbial Activity during Infection with Salmonella enterica Serovar Typhimurium.

Cells

Brigo N,Neumaier E,Pfeifhofer-Obermair C,Grubwieser P,Engl S,Berger S,Seifert M,Reinstadler V,Oberacher H,Weiss G

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Fri Apr 14 00:00:00 EDT 2023

Repurposing nitric oxide donating drugs in cancer therapy through immune modulation.

Journal of experimental & clinical cancer research : CR

Li CY,Anuraga G,Chang CP,Weng TY,Hsu HP,Ta HDK,Su PF,Chiu PH,Yang SJ,Chen FW,Ye PH,Wang CY,Lai MD

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Sat Jan 14 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Invasive margin tissue-resident macrophages of high CD163 expression impede responses to T cell-based immunotherapy.

Journal for immunotherapy of cancer

van Elsas MJ,Labrie C,Etzerodt A,Charoentong P,van Stigt Thans JJC,Van Hall T,van der Burg SH

25-3697-82 was used in Flow Cytometry to identify a small but discernible CD163hi macrophage population, with high expression of several tumor-promoting macrophage markers and a functional anti-inflammatory transcriptome profile, but not other macrophages, to be responsible.

Wed Mar 01 00:00:00 EST 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Tue Mar 14 00:00:00 EDT 2023

Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages.

Cellular & molecular biology letters

Gao T,Huang F,Wang W,Xie Y,Wang B

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Thu Mar 17 00:00:00 EDT 2022

Angiotensin 1-7 and its analogue decrease blood pressure but aggravate renal damage in preeclamptic mice.

Experimental animals

Liu Y,Zhai R,Tong J,Yu Y,Yang L,Gu Y,Niu J

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Thu Nov 10 00:00:00 EST 2022

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Molecular medicine (Cambridge, Mass.)

Wu HY,Liu K,Zhang JL

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Sat Sep 24 00:00:00 EDT 2022

Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNF.

Life science alliance

Dichtl S,Sanin DE,Koss CK,Willenborg S,Petzold A,Tanzer MC,Dahl A,Kabat AM,Lindenthal L,Zeitler L,Satzinger S,Strasser A,Mann M,Roers A,Eming SA,El Kasmi KC,Pearce EJ,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2022

Spatiotemporal dynamics of macrophage heterogeneity and a potential function of Trem2^hi macrophages in infarcted hearts.

Nature communications

Jung SH,Hwang BH,Shin S,Park EH,Park SH,Kim CW,Kim E,Choo E,Choi IJ,Swirski FK,Chang K,Chung YJ

25-3697-82 was used in Flow cytometry/Cell sorting to investigate the spatiotemporal dynamics of myocardial infarction (MI)-associated immune cells in a mouse model of MI using spatial transcriptomics and single-cell RNA-sequencing (scRNA-seq).

Sat Aug 06 00:00:00 EDT 2022

BCG hydrogel promotes CTSS-mediated antigen processing and presentation, thereby suppressing metastasis and prolonging survival in melanoma.

Journal for immunotherapy of cancer

Kremenovic M,Chan AA,Feng B,Bäriswyl L,Robatel S,Gruber T,Tang L,Lee DJ,Schenk M

25-3697-82 was used in Flow cytometry/Cell sorting to provide mechanistic insights as well as rationale for the clinical translation of BCG hydrogel as cancer immunotherapy to overcome the current limitations of immunotherapies for the treatment of patients with melanoma.

Wed Jun 01 00:00:00 EDT 2022

Helicase antigen (HAGE)-derived vaccines induce immunity to HAGE and ImmunoBody®-HAGE DNA vaccine delays the growth and metastasis of HAGE-expressing tumors in vivo.

Immunology and cell biology

Nagarajan D,Pearson J,Brentville V,Metheringham R,Pockley AG,Durrant L,McArdle SE

25-3697-82 was used in Flow cytometry/Cell sorting to demonstrate the potential of HAGEderived vaccines for treating HAGE-expressing cancers and that such vaccines could be considered as therapeutic options for patients with TNBC expressing HAGE after conventional treatment to prevent disease recurrence.

Fri Oct 01 00:00:00 EDT 2021

Lactate and IL6 define separable paths of inflammatory metabolic adaptation.

Science advances

Dichtl S,Lindenthal L,Zeitler L,Behnke K,Schlösser D,Strobl B,Scheller J,El Kasmi KC,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Dissecting the microenvironment around biosynthetic scaffolds in murine skin wound healing.

Science advances

Hu C,Chu C,Liu L,Wang C,Jin S,Yang R,Rung S,Li J,Qu Y,Man Y

25-3697 was used in Flow cytometry/Cell sorting to evaluate the effect of electrospun membranes on wound healing and immunomodulatory properties.

Sat May 01 00:00:00 EDT 2021

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Immunohistochemistry

Fri Jul 17 00:00:00 EDT 2020

Chinese Medicine Formula PSORI-CM02 Alleviates Psoriatic Dermatitis via M-MDSCs and Th17 Crosstalk.

Frontiers in pharmacology

Deng J,Tan S,Liu R,Yu W,Chen H,Tang N,Han L,Lu C

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Sat Feb 06 00:00:00 EST 2021

Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.

Cell death & disease

Hu Q,Peng J,Jiang L,Li W,Su Q,Zhang J,Li H,Song M,Cheng B,Xia J,Wu T

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Wed Oct 28 00:00:00 EDT 2020

Regulation of the tumor immune microenvironment and vascular normalization in TNBC murine models by a novel peptide.

Oncoimmunology

Mirando AC,Patil A,Rafie CI,Christmas BJ,Pandey NB,Stearns V,Jaffee EM,Roussos Torres ET,Popel AS

Published figure using Arginase 1 monoclonal antibody (Product # 25-3697-82) in Flow Cytometry

Thu May 14 00:00:00 EDT 2020

Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.

Nature communications

Kurelac I,Iommarini L,Vatrinet R,Amato LB,De Luise M,Leone G,Girolimetti G,Umesh Ganesh N,Bridgeman VL,Ombrato L,Columbaro M,Ragazzi M,Gibellini L,Sollazzo M,Feichtinger RG,Vidali S,Baldassarre M,Foriel S,Vidone M,Cossarizza A,Grifoni D,Kofler B,Malanchi I,Porcelli AM,Gasparre G

25-3697-82 was used in Flow Cytometry to demonstrate that respiratory complex I-deficient tumours survive and carry out angiogenesis, despite their inability to stabilise Hypoxia Inducible Factor-1 alpha.

Fri Feb 22 00:00:00 EST 2019

生物信息学

蛋白别名: A-I; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

查看更多收起

基因别名: AI; AI256583; Arg-1; ARG1; PGIF

查看更多收起

UniProt ID: (Human) P05089, (Mouse) Q61176

查看更多收起

Entrez Gene ID: (Human) 383, (Mouse) 11846

查看更多收起

功能

arginase activity manganese ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines metal ion binding hydrolase

参与通路