Invitrogen

ROR gamma (t) Monoclonal Antibody (B2D), PE-Cyanine7, eBioscience™

ROR gamma (t) Antibody in Flow Cytometry (Flow)

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ROR gamma (t) Antibody (25-6981-82) in Flow

C57BL/6 mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD4 Monoclonal Antibody, FITC (Product # 11-0041-82) and 0.25 µg of Rat IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4301-82) (left) or 0.25 µg of ROR gamma (t) Monoclonal Antibody, PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

ROR gamma (t) Antibody in Western Blot (WB)

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产品信息

25-6981-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

B2D

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2784671

产品详细信息

Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.

Applications Reported: This B2D antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B2D antibody has been tested by flow cytometric analysis of mouse thymocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

靶标信息

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.

ROR gammat t (RORgt) is an isoform of RORC.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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25-6981-82 was used in Flow cytometry/Cell sorting to conclude that vaccination eliminated CCR6+ TCF1+ cells and dramatically improved the subdominant response, highlighting a strategy to optimally engage concurrent neoantigen responses against tumors.

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25-6981-82 was used in Flow cytometry/Cell sorting to utilize non-redundant pathways to preserve metabolic integrity.

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Tipping the immunostimulatory and inhibitory DAMP balance to harness immunogenic cell death.

Nature communications

Hayashi K,Nikolos F,Lee YC,Jain A,Tsouko E,Gao H,Kasabyan A,Leung HE,Osipov A,Jung SY,Kurtova AV,Chan KS

Published figure using ROR gamma (t) monoclonal antibody (Product # 25-6981-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

生物信息学

蛋白别名: MGC129539; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; RORgt; RP11-98D18.11-001; Thymus orphan receptor; TOR

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基因别名: Nr1f3; Rorc; Rorg; RORgamma; Thor; TOR

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UniProt ID: (Mouse) P51450

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Entrez Gene ID: (Mouse) 19885

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功能