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Invitrogen
Claudin 4 Monoclonal Antibody (3E2C1)
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FIGURE: 1 / 4
Claudin 4 Antibody (32-9400) in WB
Western blot was performed using Anti- Claudin 4 Monoclonal Antibody (3E2C1) (Product # 32-9400) and a 18 kDa band corresponding to CLDN4 was observed across the positive cells lines tested (MCF7 and HT-29) and not in the negative models (A549 and HeLa) as reported. Membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), HT-29 (Lane 3), A549 (Lane 2) and HeLa (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB230... View More
Product Details
32-9400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Amphibian,
Bovine,
C. elegans,
Dog,
Fish,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Virus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3E2C1
Immunogen
Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin-4.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reactivity has been confirmed with human, rat ileum homogenates, and canine MDCK cell lysates by western blotting and immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry.
For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.
This antibody reacts specifically with the ~ 22 kDa Claudin-4 protein and does not cross-react with the protein at 55 kDa.
Target Information
Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Claudin-4; CLAUDIN4; Clostridium perfringens enterotoxin receptor; Clostridium perfringens enterotoxin receptor 1; CPE-R; CPE-receptor; h CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein
Gene Aliases: Cep-r; CLDN4; CPE-R; CPER; CPETR; CPETR1; hCPE-R; WBSCR8
UniProt ID: (Human) O14493, (Dog) A0A8C0N7E5, (Mouse) O35054
Entrez Gene ID: (Human) 1364, (Dog) 100856416, (Rat) 304407, (Mouse) 12740
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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