Invitrogen

OCT3/4 Monoclonal Antibody (EM92), eFluor™ 570, eBioscience™

OCT3/4 Antibody in Immunocytochemistry (ICC/IF)

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OCT3/4 Antibody (41-5841-80) in ICC/IF

Immunocytochemistry on fixed and permeabilized F9 cells using 5 µg/mL Rat IgG2a K Isotype Control eFluor® 570 (left) or 5 µg/mL Anti-Human/Mouse OCT3/4 eFluor® 570 (right). Nuclei are stained with DAPI. Colocalization of OCT3/4 and DAPI appears pink.

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产品信息

41-5841-80

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

EM92

偶联物

eFluor™ 570 eFluor™ 570 eFluor™ 570

激发/发射光谱

556/569 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11219675

产品详细信息

Description: The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).

Applications Reported: This EM92 antibody has been reported for use in immunocytochemistry.

Applications Tested: This EM92 antibody has been tested by immunocytochemistry on fixed and permeabilized F9 cells at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

靶标信息

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution.

Cell reports methods

Bertan F,Wischhof L,Scifo E,Guranda M,Jackson J,Marsal-Cots A,Piazzesi A,Stork M,Peitz M,Prehn JHM,Ehninger D,Nicotera P,Bano D

Published figure using OCT3/4 monoclonal antibody (Product # 41-5841-82) in Immunocytochemistry

Mon May 24 00:00:00 EDT 2021

Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates.

Cell reports

Patananan AN,Sercel AJ,Wu TH,Ahsan FM,Torres A,Kennedy SAL,Vandiver A,Collier AJ,Mehrabi A,Van Lew J,Zakin L,Rodriguez N,Sixto M,Tadros W,Lazar A,Sieling PA,Nguyen TL,Dawson ER,Braas D,Golovato J,Cisneros L,Vaske C,Plath K,Rabizadeh S,Niazi KR,Chiou PY,Teitell MA

Published figure using OCT3/4 monoclonal antibody (Product # 41-5841-82) in Immunocytochemistry

Tue Dec 29 00:00:00 EST 2020

生物信息学

蛋白别名: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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基因别名: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

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UniProt ID: (Human) Q01860, (Mouse) P20263

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Entrez Gene ID: (Human) 5460, (Mouse) 18999

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功能