Invitrogen

CD4 Monoclonal Antibody (RM4-5), eFluor™ 615, eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

14个已发表图片

17 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD4 Antibody in Immunohistochemistry (Frozen) (IHC (F))

图: 1 / 16

CD4 Antibody (42-0042-82) in IHC (F)

Immunohistochemistry on frozen mouse spleen using 1 µg/mL Anti-Mouse CD4 eFluor® 615. Nuclei are counterstained with DAPI.

CD4 Antibody in Immunocytochemistry (ICC/IF)

CD4 Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))

CD4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD4 Antibody — Staining of mouse splenocytes and bone marrow cells. Right: As expected based on known relative expression patterns, CD4 clone RM4-5 stains a subset of splenocytes and does not stain any bone marrow cells. Details: Balb/c bone marrow cells (left) and splenocytes (middle) were surface stained with CD4 (clone RM4-5) followed by staining with 7-AAD. Viable bone marrow cells in the lymphoid (blue histogram) and myeloid (purple histogram) gates and viable splenocytes (orange histogram) were used for analysis. {RE}

产品信息

42-0042-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看11篇已发表文章 11篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

1 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-Dependent

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

RM4-5

偶联物

eFluor™ 615 eFluor™ 615 eFluor™ 615

激发/发射光谱

595/614 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10853973

产品详细信息

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported: This RM4-5 antibody has been reported for use in immunocytochemical and immunohistochemical staining of frozen tissue sections (IHC-F).

Applications Tested: This Rm4-5 antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

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基因别名: Cd4; L3T4; Ly-4

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UniProt ID: (Mouse) P06332

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Entrez Gene ID: (Mouse) 12504

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功能

glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity

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