Invitrogen

CD27 Monoclonal Antibody (O323), PerCP-eFluor™ 710, eBioscience™

图: 1 / 18

CD27 Antibody (46-0279-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD27 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

46-0279-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

查看16篇已发表文章 16篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

O323

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1834391

产品详细信息

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.

Life science alliance

Verhoeven D,Grinwis L,Marsman C,Jansen MH,Van Leeuwen EM,Kuijpers TW

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Oxygen level is a critical regulator of human B cell differentiation and IgG class switch recombination.

Frontiers in immunology

Koers J,Marsman C,Steuten J,Tol S,Derksen NIL,Ten Brinke A,van Ham SM,Rispens T

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses.

Vaccines

Sangare K,Helmold Hait S,Moore M,Hogge C,Hoang T,Rahman MA,Venzon DJ,LaBranche C,Montefiori D,Robert-Guroff M,Thomas MA

46-0279-42 was used in Flow Cytometry to show that cells infected with an E1B55K and E4orf1-deleted (ΔE41) Ad exhibited reduced levels of phosphorylated Akt (Ser473 and Thr308)) and expressed different intrinsic innate immune cytokines from those induced in cells infected with an E4orf1-containing, ΔE1B parental Ad that exhibited elevated levels of phosphorylated Akt.

Tue Feb 15 00:00:00 EST 2022

A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques.

Journal of virology

Musich T,Thovarai V,Venzon DJ,Mohanram V,Tuero I,Miller-Novak LK,Helmold Hait S,Rahman MA,Hunegnaw R,Huiting E,Yuan W,O'hUigin C,Hoang T,Sui Y,LaBranche C,Montefiori D,Bear J,Rosati M,Bissa M,Berzofsky JA,Pavlakis GN,Felber BK,Franchini G,Robert-Guroff M

46-0279-42 was used in Flow Cytometry to highlight the critical importance of considering effects of sex and the microbiome in vaccine design and evaluation.

Mon Nov 23 00:00:00 EST 2020

NKG2C^pos NK Cells Regulate the Expansion of Cytomegalovirus-Specific CD8 T Cells.

Journal of immunology (Baltimore, Md. : 1950)

Grutza R,Moskorz W,Senff T,Bäcker E,Lindemann M,Zimmermann A,Uhrberg M,Lang PA,Timm J,Cosmovici C

46-0279-42 was used in Flow Cytometry to show that NKG2Cpos NK cells interact with HLA-Ehigh CD8 T cells, which may negatively regulate the expansion of CMV-specific CD8 T cells upon activation.

Mon Jun 01 00:00:00 EDT 2020

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

46-0279 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

46-0279 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 EST 2020

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Perturbed CD8⁺ T cell immunity across universal influenza epitopes in the elderly.

Journal of leukocyte biology

Nguyen THO,Sant S,Bird NL,Grant EJ,Clemens EB,Koutsakos M,Valkenburg SA,Gras S,Lappas M,Jaworowski A,Crowe J,Loh L,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Maintenance of the EBV-specific CD8⁺ TCRαβ repertoire in immunosuppressed lung transplant recipients.

Immunology and cell biology

Nguyen TH,Bird NL,Grant EJ,Miles JJ,Thomas PG,Kotsimbos TC,Mifsud NA,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Loss of marginal zone B-cells in SHIVSF162P4 challenged rhesus macaques despite control of viremia to low or undetectable levels in chronic infection.

Virology

Demberg T,Mohanram V,Musich T,Brocca-Cofano E,McKinnon KM,Venzon D,Robert-Guroff M

46-0279-42 was used in Flow Cytometry to conclude that MZ B-cell dysregulation and dysfunction associated with SIV/HIV infection are not readily reversible.

Thu Oct 01 00:00:00 EDT 2015

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.

Biotechnology and bioengineering

Kuzin I,Sun H,Moshkani S,Feng C,Mantalaris A,Wu JH,Bottaro A

Published figure using CD27 monoclonal antibody (Product # 46-0279-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2011

生物信息学

蛋白别名: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

查看更多收起

基因别名: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

查看更多收起

UniProt ID: (Human) P26842

查看更多收起

Entrez Gene ID: (Human) 939

查看更多收起

功能

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process

参与通路