Invitrogen

MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), PerCP-eFluor™ 710, eBioscience™

MHC Class II I-Ab Antibody in Flow Cytometry (Flow)

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MHC Class II I-Ab Antibody (46-5320-82) in Flow

Staining of C57Bl/6 splenocytes with 0.25 µg of Mouse IgG2a kappa Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (blue histogram) or 0.25 µg of Anti-Mouse MHC Class II I-Ab PerCP-eFluor® 710 (purple histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

46-5320-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

AF6-120.1

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10698173

产品详细信息

Description: This AF6-120.1 monoclonal antibody reacts with the mouse MHC Class II I-Ab alloantigen of H-2b bearing mouse strains, including C57Bl/6 and 129. This cell surface molecule is involved in antigen presentation to T cells expressing CD3/TCR and CD4.

The AF6-120.1 antibody crossreacts with H-2k and H-2u haplotypes. However, reactivity to other haplotypes (i.e., d, f, g7, p, q, r, and s) has not been observed.

Applications Reported: This AF6-120.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF6-120.1 antibody has been tested by flow cytometric analysis of C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

I-Ab (H2-Ab1, histocompatibility 2, class II antigen A, beta 1) is an MHC class II heterodimer molecule of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II. H2-Ab1 heterodimer chains alpha (DQA) and beta (DQB) are both anchored in the membrane. H2-Ab1 plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for polymorphisms is routinely done for bone marrow transplantation.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CELIAC1; DADB-249P12.2; H-2 class II histocompatibility antigen, A beta chain; H-2Eb; H2Eb; histocompatibility 2, class II antigen A, beta 1; HLA-DQB; Ia-4; Ia4; IDDM1; major histocompatibility complex class II beta chain; MGC163794; MGC163796; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1

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基因别名: Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1

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UniProt ID: (Mouse) P14483

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Entrez Gene ID: (Mouse) 14961

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功能

protein binding transcription factor binding toxic substance binding ubiquitin protein ligase binding peptide antigen binding protein heterodimerization activity repressing transcription factor binding major histocompatibility complex protein

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