Invitrogen

Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), PerCP-eFluor™ 710, eBioscience™

Dendritic Cell Marker DCIR2 Antibody in Flow Cytometry (Flow)

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Dendritic Cell Marker DCIR2 Antibody (46-5884-80) in Flow

Staining of mouse splenocytes with Anti-Mouse MHC Class II (I-A/I-E) eFluor® 450 (Product # 48-5321) and 0.125 µg of Rat IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4031) (left) or 0.125 µg of Anti-Mouse Dendritic Cell Marker DCIR2 PerCP-eFluor® 710 (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866), were used for analysis.

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产品信息

46-5884-80

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

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流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

33D1

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573790

产品详细信息

Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.^M
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Applications Reported: This 33D1 antibody has been reported for use in flow cytometric analysis.^M
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Applications Tested: This 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.^M
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PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.^M
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Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.^M
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Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.^M
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Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.^M
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Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

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