Invitrogen

CD45RA Monoclonal Antibody (HI100), APC-eFluor™ 780, eBioscience™

CD45RA Antibody in Flow Cytometry (Flow)

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CD45RA Antibody (47-0458-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RO PerCP-eFluor® 710 (Product # 46-0457-42) and Mouse IgG2b K Isotype Control APC-eFluor® 780 (Product # 47-4732-80) (left) or Anti-Human CD45RA APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

47-0458-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看16篇已发表文章 16篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

HI100

偶联物

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10853641

产品详细信息

Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

Differentiation into an Effector Memory Phenotype Potentiates HIV-1 Latency Reversal in CD4⁺ T Cells.

Journal of virology

Kulpa DA,Talla A,Brehm JH,Ribeiro SP,Yuan S,Bebin-Blackwell AG,Miller M,Barnard R,Deeks SG,Hazuda D,Chomont N,Sékaly RP

47-0458-42 was used in Flow Cytometry to support the idea that the effector memory phenotype supports HIV-1 latency reversal in CD4+ T cells.

Sun Dec 15 00:00:00 EST 2019

MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

Lv M,Li Z,Liu J,Lin F,Zhang Q,Li Z,Wang Y,Wang K,Xu Y

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia.

Nature communications

Bell CC,Fennell KA,Chan YC,Rambow F,Yeung MM,Vassiliadis D,Lara L,Yeh P,Martelotto LG,Rogiers A,Kremer BE,Barbash O,Mohammad HP,Johanson TM,Burr ML,Dhar A,Karpinich N,Tian L,Tyler DS,MacPherson L,Shi J,Pinnawala N,Yew Fong C,Papenfuss AT,Grimmond SM,Dawson SJ,Allan RS,Kruger RG,Vakoc CR,Goode DL,Naik SH,Gilan O,Lam EYN,Marine JC,Prinjha RK,Dawson MA

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Thu Jun 20 00:00:00 EDT 2019

Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators.

Cell reports

Lagou V,Garcia-Perez JE,Smets I,Van Horebeek L,Vandebergh M,Chen L,Mallants K,Prezzemolo T,Hilven K,Humblet-Baron S,Moisse M,Van Damme P,Boeckxstaens G,Bowness P,Dubois B,Dooley J,Liston A,Goris A

47-0458-42 was used in Flow Cytometry to identify eight genome-wide significant associations explaining 6%-20% of variance in immune system regulation.

Tue Oct 16 00:00:00 EDT 2018

Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors.

Nature communications

Duhen T,Duhen R,Montler R,Moses J,Moudgil T,de Miranda NF,Goodall CP,Blair TC,Fox BA,McDermott JE,Chang SC,Grunkemeier G,Leidner R,Bell RB,Weinberg AD

47-0458 was used in Flow cytometry/Cell sorting to develop an approach for detecting tumour-reactive tumour-infiltrating CD8 T cells that will help define mechanisms of existing immunotherapy treatments.

Fri Jul 13 00:00:00 EDT 2018

UM171 Enhances Lentiviral Gene Transfer and Recovery of Primitive Human Hematopoietic Cells.

Molecular therapy. Methods & clinical development

Ngom M,Imren S,Maetzig T,Adair JE,Knapp DJHF,Chagraoui J,Fares I,Bordeleau ME,Sauvageau G,Leboulch P,Eaves C,Humphries RK

47-0458 was used in Flow cytometry/Cell sorting to examine the effect of the haematopoietic stem cell agonist UM171 on the gene transfer efficiencies and yields of transplantable transduced human haematopoietic stem cells both in vivo and in vitro.

Fri Sep 21 00:00:00 EDT 2018

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection.

Nature

Eyquem J,Mansilla-Soto J,Giavridis T,van der Stegen SJ,Hamieh M,Cunanan KM,Odak A,Gönen M,Sadelain M

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Thu Mar 02 00:00:00 EST 2017

HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy.

AIDS (London, England)

Gosselin A,Wiche Salinas TR,Planas D,Wacleche VS,Zhang Y,Fromentin R,Chomont N,Cohen ÉA,Shacklett B,Mehraj V,Ghali MP,Routy JP,Ancuta P

47-0458 was used in Flow cytometry/Cell sorting to investigate the contribution of colon and blood CCR6+CD4+ T-cells to HIV persistence during antiretroviral therapy.

Mon Jan 02 00:00:00 EST 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using CD45RA monoclonal antibody (Product # 47-0458-42) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways.

Genome biology

Psaila B,Barkas N,Iskander D,Roy A,Anderson S,Ashley N,Caputo VS,Lichtenberg J,Loaiza S,Bodine DM,Karadimitris A,Mead AJ,Roberts I

47-0458 was used in Flow cytometry/Cell sorting to clarify the cellular hierarchy in human megakaryocyte/erythroid lineage commitment and highlight the importance of using a combination of single-cell approaches to dissect cellular heterogeneity.

Tue May 03 00:00:00 EDT 2016

Variable activation of the DNA damage response pathways in patients undergoing single-photon emission computed tomography myocardial perfusion imaging.

Circulation. Cardiovascular imaging

Lee WH,Nguyen P,Hu S,Liang G,Ong SG,Han L,Sanchez-Freire V,Lee AS,Vasanawala M,Segall G,Wu JC

47-0458 was used in Flow cytometry/Cell sorting to analyse changes in protein phosphorylation and DNA damage response gene expression to evaluate low-dose radiation risk in patients subjected to SPECT MPI.

Sun Feb 01 00:00:00 EST 2015

生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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功能

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

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