Invitrogen

FceR1 alpha Monoclonal Antibody (MAR-1), APC-eFluor™ 780, eBioscience™

FceR1 alpha Antibody in Flow Cytometry (Flow)

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FceR1 alpha Antibody (47-5898-82) in Flow

Staining of MC/9 cells with 0.25 µg of Armenian Hamster IgG Isotype Control APC-eFluor® 780 (Product # 47-4888-80) (blue histogram) or 0.25 µg of Anti-Mouse Fc epsilon Receptor I alpha (FceR1) APC-eFluor® 780 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 520 (Product # 65-0867-14), were used for analysis.

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产品信息

47-5898-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看13篇已发表文章 13篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

MAR-1

偶联物

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573990

产品详细信息

Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported: This MAR-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of Endometriosis.

Frontiers in immunology

Guo X,Xu X,Li T,Yu Q,Wang J,Chen Y,Ding S,Zhu L,Zou G,Zhang X

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Tue Jan 18 00:00:00 EST 2022

SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.

The Journal of biological chemistry

Cardenas RA,Gonzalez R,Sanchez E,Ramos MA,Cardenas EI,Rodarte AI,Alcazar-Felix RJ,Isaza A,Burns AR,Heidelberger R,Adachi R

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Mutant Huntingtin affects toll-like receptor 4 intracellular trafficking and cytokine production in mast cells.

Journal of neuroinflammation

Pérez-Rodríguez MJ,Ibarra-Sánchez A,Román-Figueroa A,Pérez-Severiano F,González-Espinosa C

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Fri Mar 27 00:00:00 EDT 2020

Tespa1 plays a role in the modulation of airway hyperreactivity through the IL-4/STAT6 pathway.

Journal of translational medicine

Yang R,Wang G,Li L,He H,Zheng M,Lu L,Wu S

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Mon Nov 23 00:00:00 EST 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Type I interferon limits mast cell-mediated anaphylaxis by controlling secretory granule homeostasis.

PLoS biology

Kobayashi T,Shimabukuro-Demoto S,Tsutsui H,Toyama-Sorimachi N

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

47-5898 was used in Flow cytometry/Cell sorting to show that progranulin (PGRN) can reduce NK cell cytotoxicity through reduction of NK cell expansion, granzyme B transcription, and NK cell-mediated lysis of target cells.

Thu Sep 05 00:00:00 EDT 2019

Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.

Frontiers in immunology

Potuckova L,Draberova L,Halova I,Paulenda T,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

Guidelines for the use of flow cytometry and cell sorting in immunological studies.

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Akdis M,Andrä I,Annunziato F,Bacher P,Barnaba V,Battistini L,Bauer WM,Baumgart S,Becher B,Beisker W,Berek C,Blanco A,Borsellino G,Boulais PE,Brinkman RR,Büscher M,Busch DH,Bushnell TP,Cao X,Cavani A,Chattopadhyay PK,Cheng Q,Chow S,Clerici M,Cooke A,Cosma A,Cosmi L,Cumano A,Dang VD,Davies D,De Biasi S,Del Zotto G,Della Bella S,Dellabona P,Deniz G,Dessing M,Diefenbach A,Di Santo J,Dieli F,Dolf A,Donnenberg VS,Dörner T,Ehrhardt GRA,Endl E,Engel P,Engelhardt B,Esser C,Everts B,Dreher A,Falk CS,Fehniger TA,Filby A,Fillatreau S,Follo M,Förster I,Foster J,Foulds GA,Frenette PS,Galbraith D,Garbi N,García-Godoy MD,Geginat J,Ghoreschi K,Gibellini L,Goettlinger C,Goodyear CS,Gori A,Grogan J,Gross M,Grützkau A,Grummitt D,Hahn J,Hammer Q,Hauser AE,Haviland DL,Hedley D,Herrera G,Herrmann M,Hiepe F,Holland T,Hombrink P,Houston JP,Hoyer BF,Huang B,Hunter CA,Iannone A,Jäck HM,Jávega B,Jonjic S,Juelke K,Jung S,Kaiser T,Kalina T,Keller B,Khan S,Kienhöfer D,Kroneis T,Kunkel D,Kurts C,Kvistborg P,Lannigan J,Lantz O,Larbi A,LeibundGut-Landmann S,Leipold MD,Levings MK,Litwin V,Liu Y,Lohoff M,Lombardi G,Lopez L,Lovett-Racke A,Lubberts E,Ludewig B,Lugli E,Maecker HT,Martrus G,Matarese G,Maueröder C,McGrath M,McInnes I,Mei HE,Melchers F,Melzer S,Mielenz D,Mills K,Mirrer D,Mjösberg J,Moore J,Moran B,Moretta A,Moretta L,Mosmann TR,Müller S,Müller W,Münz C,Multhoff G,Munoz LE,Murphy KM,Nakayama T,Nasi M,Neudörfl C,Nolan J,Nourshargh S,O'Connor JE,Ouyang W,Oxenius A,Palankar R,Panse I,Peterson P,Peth C,Petriz J,Philips D,Pickl W,Piconese S,Pinti M,Pockley AG,Podolska MJ,Pucillo C,Quataert SA,Radstake TRDJ,Rajwa B,Rebhahn JA,Recktenwald D,Remmerswaal EBM,Rezvani K,Rico LG,Robinson JP,Romagnani C,Rubartelli A,Ruckert B,Ruland J,Sakaguchi S,Sala-de-Oyanguren F,Samstag Y,Sanderson S,Sawitzki B,Scheffold A,Schiemann M,Schildberg F,Schimisky E,Schmid SA,Schmitt S,Schober K,Schüler T,Schulz AR,Schumacher T,Scotta C,Shankey TV,Shemer A,Simon AK,Spidlen J,Stall AM,Stark R,Stehle C,Stein M,Steinmetz T,Stockinger H,Takahama Y,Tarnok A,Tian Z,Toldi G,Tornack J,Traggiai E,Trotter J,Ulrich H,van der Braber M,van Lier RAW,Veldhoen M,Vento-Asturias S,Vieira P,Voehringer D,Volk HD,von Volkmann K,Waisman A,Walker R,Ward MD,Warnatz K,Warth S,Watson JV,Watzl C,Wegener L,Wiedemann A,Wienands J,Willimsky G,Wing J,Wurst P,Yu L,Yue A,Zhang Q,Zhao Y,Ziegler S,Zimmermann J

47-5898 was used in Flow cytometry/Cell sorting to collect the currently accepted best methods for monitoring most of the variation of the major players of immune system.

Sun Oct 01 00:00:00 EDT 2017

Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life.

Mucosal immunology

Patel KR,Aven L,Shao F,Krishnamoorthy N,Duvall MG,Levy BD,Ai X

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2016

New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling.

Molecular and cellular biology

Bambouskova M,Polakovicova I,Halova I,Goel G,Draberova L,Bugajev V,Doan A,Utekal P,Gardet A,Xavier RJ,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Isolation of Mature (Peritoneum-Derived) Mast Cells and Immature (Bone Marrow-Derived) Mast Cell Precursors from Mice.

PloS one

Meurer SK,Neß M,Weiskirchen S,Kim P,Tag CG,Kauffmann M,Huber M,Weiskirchen R

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Mon Jul 24 00:00:00 EDT 2017

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

Wed Jul 01 00:00:00 EDT 2009

IL-21R is essential for epicutaneous sensitization and allergic skin inflammation in humans and mice.

The Journal of clinical investigation

Jin H,Oyoshi MK,Le Y,Bianchi T,Koduru S,Mathias CB,Kumar L,Le Bras S,Young D,Collins M,Grusby MJ,Wenzel J,Bieber T,Boes M,Silberstein LE,Oettgen HC,Geha RS

Published figure using FceR1 alpha monoclonal antibody (Product # 47-5898-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2009

生物信息学

蛋白别名: fc epsilon r1; Fc-epsilon RI-alpha; FcERI; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; RP11-550P17.3

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基因别名: Fce1a; fcepsilonri; Fcer1a; FcERI; Fcr-5

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UniProt ID: (Mouse) P20489

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Entrez Gene ID: (Mouse) 14125

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功能

protein binding IgE receptor activity IgE binding immunoglobulin receptor superfamily

参与通路