Invitrogen

CD209a Monoclonal Antibody (MMD3), Alexa Fluor™ 488, eBioscience™

CD209a Antibody in Immunohistochemistry (Frozen) (IHC (F))

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CD209a Antibody (53-2094-82) in IHC (F)

Immunohistochemistry of frozen 5-week-old mouse thymus stained with 20 µg/mL of Mouse IgG2b K Isotype Control Alexa Fluor® 488 (left, green) or 20 µg/mL of Anti-Mouse CD209a (DC-SIGN) Alexa Fluor® 488 (right, green) and costained with 20 µg/mL of Anti-Mouse CD8a eFluor® 660 (red). Nuclei are stained with DAPI (blue).

CD209a Antibody in Flow Cytometry (Flow)

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产品信息

53-2094-82

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

20 µg/mL

免疫细胞化学 (ICC/IF)

Assay-Dependent

流式细胞分析 (Flow)

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产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2c, kappa

分类

Monoclonal

类型

Antibody

克隆号

MMD3

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2574419

产品详细信息

Description: This MMD3 monoclonal antibody recognizes mouse CD209a, which is also known as DC-SIGN. CD209a is a type II transmembrane C-type lectin expressed on a subset of dendritic cells, including some CD4+, CD8- and plasmacytoid pre-dendritic cells. Studies indicate that CD209a expression can vary according to the activation state of the host. Moreover, CD209a is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. CD209a is involved in mediating the innate immune response by binding microbial carbohydrates.

Cross-blocking studies suggest that MMD3 recognizes a different a different epitope from LWC06 and 5H10.

Applications Reported: This MMD3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This MMD3 antibody has been tested by immunohistochemistry on frozen 5-week-old mouse thymus and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

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