Invitrogen

HLA-ABC Monoclonal Antibody (W6/32), Alexa Fluor™ 700, eBioscience™

HLA-ABC Antibody in Flow Cytometry (Flow)

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HLA-ABC Antibody (56-9983-42) in Flow

Normal human peripheral blood cells were stained with Mouse IgG2a kappa Isotype Control, Alexa Fluor 700 (Product # 56-4724-80) (blue histogram) or HLA-ABC Monoclonal Antibody, Alexa Fluor 700 (purple histogram). Cells in the lymphocyte gate were used for analysis.

HLA-ABC Antibody in Neutralization (Neu)

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产品信息

56-9983-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看5篇已发表文章 5篇已发表文章

中和实验 (Neu)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

W6/32

偶联物

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

激发/发射光谱

696/719 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2811901

产品详细信息

Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey.

Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This W6/32 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser

靶标信息

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.

Nature communications

Piekarska K,Urban-Wójciuk Z,Kurkowiak M,Pelikant-Małecka I,Schumacher A,Sakowska J,Spodnik JH,Arcimowicz Ł,Zielińska H,Tymoniuk B,Renkielska A,Siebert J,Słomińska E,Trzonkowski P,Hupp T,Marek-Trzonkowska NM

56-9983-42 was used in Flow Cytometry to demonstrate that both direct and indirect contact with allogeneic ASCs improves therapeutic potential of Tregs (Regulatory T cells).

Mon Feb 14 00:00:00 EST 2022

Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.

Nature communications

56-9983-42 was used in Flow Cytometry to demonstrate that both direct and indirect contact with allogeneic ASCs improves therapeutic potential of Tregs (Regulatory T cells).

Mon Feb 14 00:00:00 EST 2022

Capturing T Lymphocytes' Dynamic Interactions With Human Neural Cells Using Time-Lapse Microscopy.

Frontiers in immunology

Lemaître F,Carmena Moratalla A,Farzam-Kia N,Carpentier Solorio Y,Tastet O,Cleret-Buhot A,Guimond JV,Haddad E,Arbour N

Published figure using HLA-ABC monoclonal antibody (Product # 56-9983-42) in Neutralization

Wed Oct 20 00:00:00 EDT 2021

Frequent amplification of HDAC genes and efficacy of HDAC inhibitor chidamide and PD-1 blockade combination in soft tissue sarcoma.

Journal for immunotherapy of cancer

Que Y,Zhang XL,Liu ZX,Zhao JJ,Pan QZ,Wen XZ,Xiao W,Xu BS,Hong DC,Guo TH,Shen LJ,Fan WJ,Chen HY,Weng DS,Xu HR,Zhou PH,Zhang YZ,Niu XH,Zhang X

Published figure using HLA-ABC monoclonal antibody (Product # 56-9983-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Attenuation of Heparan Sulfate Proteoglycan Binding Enhances In Vivo Transduction of Human Primary Hepatocytes with AAV2.

Molecular therapy. Methods & clinical development

Cabanes-Creus M,Westhaus A,Navarro RG,Baltazar G,Zhu E,Amaya AK,Liao SHY,Scott S,Sallard E,Dilworth KL,Rybicki A,Drouyer M,Hallwirth CV,Bennett A,Santilli G,Thrasher AJ,Agbandje-McKenna M,Alexander IE,Lisowski L

Published figure using HLA-ABC monoclonal antibody (Product # 56-9983-42) in Flow Cytometry

Fri Jun 12 00:00:00 EDT 2020

Exploration of the Combination of PLK1 Inhibition with Immunotherapy in Cancer Treatment.

Journal of oncology

Li M,Liu Z,Wang X

Published figure using HLA-ABC monoclonal antibody (Product # 56-9983-42) in Flow Cytometry

Thu Mar 31 00:00:00 EDT 2022

Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs.

Oncotarget

Yuan D,Xia M,Meng G,Xu C,Song Y,Wei J

Published figure using HLA-ABC monoclonal antibody (Product # 56-9983-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2015

生物信息学

蛋白别名: A29 protein; alpha chain of MHC class II; antigen presenting molecule; class I MHC, HLA-B antigen; epididymis secretory protein Li 83; H-2K; H-2K(d); H2-K; histocompatibility antigen; HLA -B; HLA A; HLA B-40011; HLA B44; HLA C; HLA class I antigen; HLA class I antigen HLA-B; HLA class I histocompatibility antigen C alpha chain; HLA class I histocompatibility antigen Cw-1 alpha chain; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, B-47 alpha chain; HLA class I histocompatibility antigen, B-50 alpha chain; HLA class I histocompatibility antigen, B-82 alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA class I histocompatibility antigen, Cw-15 alpha chain; HLA class I histocompatibility antigen, Cw-18 alpha chain; HLA locus; HLA molecule; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-Aw33.1; HLA-B; HLA-B 1502; HLA-B alpha chain; HLA-B alpha chain (B*2706); HLA-B alpha chain (B*5002); HLA-B alpha-chain; HLA-B35; HLA-B35 antigen; HLA-Bw50 antigen; HLA-C; HLA-C alpha chain; HLA-C alpha chain (HLA-Cw*15v); HLA-C alpha chain for Cw*18GB; HLA-C alpha-chain; HLA-C antigen; HLA-Cw; HLA-Cw*04GB; HLA-Cw*1702; HLA-Cw*18GB; HLA-Cw-1503; Human leukocyte antigen A; Human leukocyte antigen B; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; hypothetical protein; lecocyte antigen B; leucocyte antigen A; Leukocyte Antigen; leukocyte antigen C; leukocyte antigen class I-A; leukocyte antigen class I-B; Leukoocyte Antigen; lymphocyte antigen; major histocompatability complex B antigen; major histocompatibility antigen HLA-C; MCH class I antigen; MGC184092; MGC7052; MHC 1; MHC antigen; MHC clas I antigen; MHC Class 1; MHC class 1 antigen; MHC class I; MHC class I alpha chain; MHC class I anitgen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen B*47; MHC class I antigen B*50; MHC class I antigen B*82; MHC class I antigen Cw*15; MHC class I antigen Cw*18; MHC class I antigen GN00104; MHC class I antigen heavy chain; MHC class I antigen heavy chain HLA-C; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen null protein; MHC class I antigen SHCHA; MHC class I antigene; MHC class I histocompatibility antigen; MHC class I human leukocyte antigen; MHC class I molecule; MHC class I protein; MHC class II antigen; MHC class l antigen; MHC classI antigen; MHC HLA-ABC; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC HLA-B27-HS; MHC HLA-B27d; MHC HLA-B39N; MHC I HLA; MHC1; surface antigen; truncated class I antigen; truncated MHC class I antigen

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基因别名: AS; Aw-33; Aw-74; B-4901; B-5001; Bw-47; Bw-50; D6S204; HEL-S-83; HLA-A; HLA-A11; HLA-A33; HLA-B; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HLA-C; HLA-Cw; HLA-DQB1; HLA-DRB1; HLA-JY3; HLAA; HLAB; HLAC; HLC-C; MHC; PSORS1; SPDA1

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UniProt ID: (Human) Q9TQM6, (Human) Q31603, (Human) Q29990

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Entrez Gene ID: (Human) 3105, (Human) 3106, (Human) 3107

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功能

protein binding beta-2-microglobulin binding peptide antigen binding poly(A) RNA binding antigen binding major histocompatibility complex protein

参与通路

antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent viral process antigen processing and presentation regulation of immune response interferon-gamma-mediated signaling pathway type I interferon signaling pathway