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FOXP3 Antibody (606-5773-82) in Flow
BALB/c mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD4 Monoclonal Antibody, Alexa Fluor 647 (Product # 51-0042-82), CD25 Monoclonal Antibody, APC (Product # 17-0251-82), CD8a Monoclonal Antibody, Alexa Fluor 700 (Product # 56-0081-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 660 (Product # 606-4321-81) or 0.25 µg of Foxp3 Monoclonal Antibody, Alexa Fluor 660. Cells in the lymphocyte gate were used for analysis and gated... View More
产品信息
606-5773-82
产品规格
种属反应
Bovine,
Dog,
Cat,
Mouse,
Pig,
Rat
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
FJK-16s
偶联物
激发/发射光谱
663/691 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2896288
产品详细信息
Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.
Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.
Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (Product # 00-5523-00). The use of other fixation and staining buffers is not recommended.
Applications Reported: This FJK-16s antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This FJK-16s antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser.
靶标信息
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Forkhead box protein P3; forkhead/winged helix transcription factor 3; Foxp3; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy
基因别名: Foxp3; JM2; RGD1562112; scurfin; sf
UniProt ID: (Mouse) Q99JB6
Entrez Gene ID: (Dog) 491876, (Pig) 444998, (Bovine) 506053, (Cat) 100037405, (Mouse) 20371, (Rat) 317382
core promoter binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription corepressor activity
protein binding
histone acetyltransferase binding
protein homodimerization activity
histone deacetylase binding
sequence-specific DNA binding
metal ion binding
NFAT protein binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
molecular_function
negative regulation of transcription from RNA polymerase II promoter
B cell homeostasis
cytokine production
myeloid cell homeostasis
CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation
CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment
T cell mediated immunity
tolerance induction
tolerance induction to self antigen
regulation of immunoglobulin production
positive regulation of T cell tolerance induction
regulation of T cell anergy
positive regulation of T cell anergy
negative regulation of chronic inflammatory response
negative regulation of T cell cytokine production
positive regulation of peripheral T cell tolerance induction
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
negative regulation of cell proliferation
anatomical structure morphogenesis
positive regulation of gene expression
negative regulation of histone deacetylation
negative regulation of NF-kappaB transcription factor activity
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
negative regulation of interleukin-17 production
negative regulation of interleukin-2 production
negative regulation of interleukin-4 production
negative regulation of interleukin-5 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of interleukin-4 production
negative regulation of CREB transcription factor activity
positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation
positive regulation of transforming growth factor beta1 production
positive regulation of immature T cell proliferation in thymus
positive regulation of histone acetylation
negative regulation of histone acetylation
negative regulation of cytokine biosynthetic process
T cell activation
negative regulation of T cell proliferation
negative regulation of sequence-specific DNA binding transcription factor activity
regulatory T cell differentiation
negative regulation of interferon-gamma biosynthetic process
negative regulation of interleukin-2 biosynthetic process
positive regulation of regulatory T cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of isotype switching to IgE isotypes
regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of cytokine secretion
negative regulation of inflammatory response
negative regulation of immune response
T cell receptor signaling pathway
negative regulation of T-helper 17 cell differentiation
biological_process
response to virus
