Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Super Bright™ 645, eBioscience™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

14个已发表图片

21 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)

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CD31 (PECAM-1) Antibody (64-0319-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714-82) (blue histogram) or Anti-Human CD31 (PECAM-1) Super Bright 645 (purple histogram). Cells in the monocyte gate were used for analysis.

CD31 (PECAM-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)

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产品信息

64-0319-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

5 µL (0.06 µg)/test

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

WM-59 (WM59)

偶联物

Super Bright™ 645 Super Bright™ 645 Super Bright™ 645

激发/发射光谱

414/645 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2717093

产品详细信息

Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: This WM/59 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM/59 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Kidney research and clinical practice

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Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs.

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Both Human Hematoma Punctured from Pelvic Fractures and Serum Increase Muscle Resident Stem Cells Response to BMP9: A Multivariate Statistical Approach.

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Functionally specialized human CD4⁺ T-cell subsets express physicochemically distinct TCRs.

eLife

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Vascular Tumor Recapitulated in Endothelial Cells from hiPSCs Engineered to Express the SERPINE1-FOSB Translocation.

Cell reports. Medicine

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Matrine inhibits the development and progression of ovarian cancer by repressing cancer associated phosphorylation signaling pathways.

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生物信息学

蛋白别名: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

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基因别名: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

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UniProt ID: (Human) P16284

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Entrez Gene ID: (Human) 5175

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功能

protein binding immunoglobulin receptor superfamily

参与通路

platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development