Invitrogen

CD45.1 Monoclonal Antibody (A20), Super Bright™ 645, eBioscience™

CD45.1 Antibody in Flow Cytometry (Flow)

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CD45.1 Antibody (64-0453-82) in Flow

Staining of C56BL/6 (blue histogram) and SJL (purple histogram) splenocytes with 0.5 µg of Anti-Mouse CD45-1 Super Bright 645. Cells in the lymphocyte gate were used for analysis.

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产品信息

64-0453-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

A20

偶联物

Super Bright™ 645 Super Bright™ 645 Super Bright™ 645

激发/发射光谱

414/645 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2662430

产品详细信息

Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.

Applications Reported: This A20 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis.

Science advances

Ma L,Tang Q,Gao X,Lee J,Lei R,Suzuki M,Zheng D,Ito K,Frenette PS,Dawlaty MM

Published figure using CD45.1 monoclonal antibody (Product # 64-0453-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

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Mon Feb 01 00:00:00 EST 2021

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

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Mon Jul 05 00:00:00 EDT 2021

Allergen-Specific Treg Cells Upregulated by Lung-Stage S. japonicum Infection Alleviates Allergic Airway Inflammation.

Frontiers in cell and developmental biology

Li Z,Zhang W,Luo F,Li J,Yang W,Zhu B,Wu Q,Wang X,Sun C,Xie Y,Xu B,Wang Z,Qian F,Chen J,Wan Y,Hu W

64-0453-82 was used in Flow Cytometry to investigate the protective effects of Schistosoma japonicum on allergic airway inflammation.

Sat Jun 26 00:00:00 EDT 2021

Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling.

Cell reports

Young IC,Wu B,Andricovich J,Chuang ST,Li R,Tzatsos A,Wu RC,Wu MY

Published figure using CD45.1 monoclonal antibody (Product # 64-0453-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

Published figure using CD45.1 monoclonal antibody (Product # 64-0453-82) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

Published figure using CD45.1 monoclonal antibody (Product # 64-0453-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45.1 monoclonal antibody (Product # 64-0453-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using CD45.1 monoclonal antibody (Product # 64-0453-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editing.

Nature communications

Scharenberg SG,Poletto E,Lucot KL,Colella P,Sheikali A,Montine TJ,Porteus MH,Gomez-Ospina N

Published figure using CD45.1 monoclonal antibody (Product # 64-0453-82) in Flow Cytometry

Fri Jul 03 00:00:00 EDT 2020

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

Published figure using CD45.1 monoclonal antibody (Product # 64-0453-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

生物信息学

蛋白别名: CD45; CD45 antigen; GP180; L-CA; Leukocyte common antigen; Ly-5; LY5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein

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基因别名: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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功能

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

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