Invitrogen

CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), Super Bright™ 645, eBioscience™

CD90.1 (Thy-1.1) Antibody in Flow Cytometry (Flow)

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CD90.1 (Thy-1.1) Antibody (64-0900-82) in Flow

Staining of rat splenocytes with Anti-Rat CD3 APC (Product # 17-0030) and 0.25 µg of Mouse IgG2a kappa Isotype Control Super Bright 645 (Product # 64-4724) (left) or 0.25 µg of CD90-1 (Thy-1-1) Monoclonal Antibody, Super Bright 645 (right). Total viable cells were used for analysis.

CD90.1 (Thy-1.1) Antibody in Immunocytochemistry (ICC/IF)

CD90.1 (Thy-1.1) Antibody in Immunohistochemistry (IHC)

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产品信息

64-0900-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

HIS51

偶联物

Super Bright™ 645 Super Bright™ 645 Super Bright™ 645

激发/发射光谱

414/645 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2717097

产品详细信息

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.

Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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生物信息学

蛋白别名: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; thy-1 glycoprotein; Thy-1 membrane glycoprotein; Thy-1 protein; thymus cell antigen 1, theta; Thymus cell surface antigen

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基因别名: CD7; CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

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UniProt ID: (Mouse) P01831, (Rat) P01830

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Entrez Gene ID: (Mouse) 21838, (Rat) 24832

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功能

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding protein binding cell adhesion molecule

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