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Invitrogen
6x-His Tag Monoclonal Antibody (HIS.H8), eBioscience™
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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6x-His Tag Antibody (14-6657-82) in ICC/IF
Immunofluorescent analysis of 6x-His Tag was performed using 70% confluent HEK-293 cells transfected with V5-H3-His construct. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with 6x-His Tag Monoclonal Antibody (HIS.H8) (Product # 14-6657-82) at 5 µg/mL and Histo... View More
产品信息
14-6657-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
HIS.H8
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572898
产品详细信息
Description: This HIS.H8 monoclonal antibody reacts with the 6X polyhistidine (6X His) tag. The H-H-H-H-H-H motif is frequently fused to the N- or C-terminus of proteins to facilitate purification (e.g., nickel affinity chromatography and immunoprecipitation) and/or detection. This antibody recognizes the 6X His tag anywhere within the protein of interest (i.e., N-terminus, C-terminus, or internal).
Applications Reported: This HIS.H8 antibody has been reported for use in immunoprecipitation and western blotting.
Applications Tested: This HIS.H8 antibody has been tested by immunoblotting of reduced cell lysate expressing a His-tagged protein at less than or equal to 1 µg/ mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 6His tag; 6X His; His Tag; Histidine Tag
