Invitrogen

A33 Recombinant Rabbit Monoclonal Antibody (JE49-54)

A33 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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A33 Antibody (MA5-34867) in IHC (P)

Immunohistochemistry analysis of GPA33 in paraffin-embedded human small intestine tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with GPA33 monoclonal antibody (Product # MA5-34867) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with... View More

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产品信息

MA5-34867

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫沉淀 (IP)

1:10-1:50

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE49-54

抗原

Recombinant protein within Human GPA33 aa 203-319

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Wet ice

RRID

AB_2848775

产品详细信息

Positive Control: Human small intestine tissue, human colon tissue, human colon cancer tissue, human appendix tissue.

靶标信息

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

仅用于科研。不用于诊断过程。未经明确授权不得转售。